The migration of osteoblasts over substrata of discrete surface charge

Dames, J.E.; Causton, B.E.; Bovell, Y. P.; Davy, K. W. M.; Sturt, C.S.

doi:10.1016/0142-9612(86)90109-2

Cited by 79 publications

(41 citation statements)

References 5 publications

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“…33 Our SEM examination revealed that ROS17/ 2.8 cells on AW(24) exhibited an identical "stand-off" morphology in sharp contrast with those on HA, suggesting that AW(24) provides a favorable substrate for ROS17/2.8 cells to express better osteoblastic phenotype. 34 AW (24) enhanced AP activity in ROS17/2.8 cells 1.64-and 1.57-fold over HA and PS, respectively, at 7 days. Also, AW(24) significantly up-regulated the expression of AP and OC mRNAs in ROS17/2.8 cells compared with HA and PS over the entire 4-, 7-, and 10-day time course: The stimulatory effects were most significant at 4 and 7 days, but decreased at 10 days.…”

Section: Discussionmentioning

confidence: 94%

In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Matsuoka

Akiyama

Okada

et al. 1999

J. Biomed. Mater. Res.

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We analyzed the mechanisms of the efficient bone formation on the osteoconductive surface of apatite- and wollastonite-containing glass-ceramic (AW) by using an in vitro system. AW releases Ca ions and bonds to bone via a submicron-thick hydroxycarbonate apatite (HCA) layer. AW disks were conditioned with simulated body fluid (SBF) to grow HCA layers, and the amount of released Ca ion was regulated by modulating the conditioning time from 24 to 240 h. Surface-transformed AW disks increased alkaline phosphatase (AP) activity in osteoblastic ROS17/2.8 cells by 1.5- to threefold over unconditioned disks. AW disks conditioned for 24 h [AW(24)], which had a homogeneous, submicron-thick apatite layer and increased extracellular ionized Ca concentration ([Ca(2+)](e)) in the culture medium to the greatest extent, enhanced the AP activity the most. High [Ca(2+)](e) promoted osteogenic differentiation in ROS17/2.8 cells: It increased AP activity in a dose-dependent manner by up to 1.6-fold, and up-regulated the expression of AP, osteocalcin (OC), and transforming growth factor-beta1 mRNAs in dose- and time-dependent manners. AW(24) enhanced AP activity in ROS17/2.8 cells as much as AW disks conditioned with SBF containing serum to exhibit in vivo surface-structure changes. AW(24) increased AP activity in ROS17/2.8 cells by 1.6-fold and enhanced the expression of AP and OC mRNAs significantly, compared with sintered hydroxyapatite (HA). After implantation of AW and HA in the distal metaphyses of rabbit femurs, thin, newly formed bone lined with cuboidal, osteoblast-like cells was characteristically observed adjacent to the AW surface within 8 days. These results provide evidence for the hypothesis that AW stimulates bone formation on its surface by increasing [Ca(2+)](e) to promote the HCA layer formation and the differentiation of osteogenic cells.

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Section: Discussionmentioning

confidence: 94%

In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Matsuoka

Akiyama

Okada

et al. 1999

J. Biomed. Mater. Res.

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“…Implanted material attains and maintains contact with interfacial tissue via its surface. The growth and proliferation of osteoblastic cells on these implants are necessary for osseointegration, which is defined by an initial close apposition of bone and long-term stable bone ingrowth to the alloy surface [Albrektsson and Albrektsson, 1987;Davies, 1996;Davies et al, 1986Davies et al, , 1990. Cell adhesion, spreading and signaling are complex processes that are dependent on the chemistry of the substrate with which the cells interact [Kieswetter et al, 1996;Shah et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

Roehlecke

Witt²,

Kasper³

et al. 2001

Cells Tissues Organs

113

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A number of studies have demonstrated the pivotal role of collagen in modulating cell growth and differentiation. In bone, where the extracellular matrix is composed of approximately 85% type I collagen, cellular interaction with matrix components has been shown to be important in the regulation of the osteoblast phenotype. Preservation or enhancement of normal osteoblast function and appositional bone formation after implant placement represents a strategy that can be useful for the purpose of improving osseointegration. In order to further improve biocompatibility, we combined two known favorable compounds, namely the titanium alloy, Ti6A14V, with type I collagen. We assessed the in vitro behavior of primary osteoblasts grown on both fibrillar collagen-coated and tropocollagen-coated Ti6A14V in comparison with uncoated titanium alloy, using an improved adsorption procedure. As parameters of biocompatibility, a variety of processes, including cell attachment, spreading, cytoskeletal organization, focal contact formation, proliferation and expression of a differentiated phenotype, were investigated. Our results demonstrated for the first time that in comparison to uncoated titanium alloy, collagen-coated alloy enhanced spreading and resulted in a more rapid formation of focal adhesions and their associated stress fibers. Growing on collagen-coated Ti6A14V, osteoblasts had a higher proliferative capacity and the intracellular expression of osteopontin was upregulated compared to uncoated titanium alloy. Type I collagen-coated titanium alloy exhibits favorable effects on the initial adhesion and growth activities of osteoblasts, which is encouraging for its potential use as bone graft material. Moreover, collagen type I may serve as an excellent biocompatible carrier for osteotropic factors such as cell adhesion molecules (e.g. fibronectin) or bone-specific growth factors.

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“…Thus, polymers presenting physicochemical and/or mechanical properties as close as possible to those of the tissues into which they will be implanted are currently being developed. These properties include an adequate balance between hydrophilicity/hydrophobicity, electrical charge distribution, hardness, elasticity, and strength [18]. A relationship exists between hydrophilicity and cell adhesion.…”

Section: Cell Adhesion On Polymer Substratesmentioning

confidence: 99%

“…Only after adhesion do the cells initiate the process of spreading, division and production of new extracellular matrix [18,19]. Cell spreading is a complex process that involves modifications in cell morphology as a consequence of alterations in the cytoskeleton, thus improving interaction with the substrate.…”

Section: Bioresorbable Polymers For Tissue Engineering 229mentioning

confidence: 99%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (PLA), such as poly(L-lactic acid), poly(Dlactic acid) and poly(DL-lactic acid), as well as poly(glycolic acid) and polycaprolactone, have been extensively studied. These polymers are well known for their good biocompatibility, with their degradation products being eliminated from the body by metabolic pathways. Many reports have shown that the different PLA-based substrates do not present toxicity since the cells were found to differentiate over the different polymers, as demonstrated by the production of extracellular matrix components by various cell types. In this chapter, we describe the use of -hydroxy acids, highlighting the different forms of PLA scaffolds used as cell culture substrates and their applications in clinical practice. The chapter is divided into (1) Introduction; (2) Bioresorbable devices as cell culture substrates; (3) Cell adhesion to polymer substrates; (4) Tissue engineering and bioresorbable polymers; (5) Cell growth and proliferation on bioresorbable polymers; (6) Bioresorbable polymers for cartilage engineering; (7) Bioresorbable polymers for bone tissue engineering; (8) Bioresorbable polymers for skin tissue engineering, and (9) Conclusion.

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The migration of osteoblasts over substrata of discrete surface charge

Cited by 79 publications

References 5 publications

In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

Bioresorbable Polymers for Tissue Engineering

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