In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Matsuoka, Hiroaki; Akiyama, Haruhiko; Okada, Yoshifumi; Ito, Hiromu; Shigeno, Chohei; Konishi, Junji; Kokubo, Tadashi; Nakamura, Takashi

doi:10.1002/(sici)1097-4636(199911)47:2<176::aid-jbm7>3.0.co;2-z

Cited by 80 publications

(46 citation statements)

References 33 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…c-fos is expressed in downstream of signal transduction pathways 46) . Bioactive glass ceramic releases calcium ions and promotes the differentiation of osteoblastic ROS17/2.8 cells in vitro 17) . The present results, however, indicate that reducing the calcium concentration below physiological conditions, induced by EGTA or OCP, raised the ALP activity of ST-2 cells.…”

Section: Discussionmentioning

confidence: 99%

“…The driving force of this change stems from the decrease of the degree of supersaturation in the solution to form HA, which is the most thermodynamically stable salt at physiological conditions 15) . Previous reports have shown that bioactive glass ceramic releases Ca 2+ and promotes the differentiation of osteoblastic cells in vitro 17) . Pi are also known to play multiple roles in maintaining osteoblast-like cells 18) .…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

View full text Add to dashboard Cite

The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Maxian et al 31 reported that alkaline phosphatase could be enhanced by hydroxyapatite with low crystallinity compared with high crystallinity in a rat calvarial bone cell culture system, and hydroxyapatite with low crystallinity showed greater calcium release than hydroxyapatite with high crystallinity. On the other hand, Matsuoka et al 32 reported that increased extracellular ionized calcium concentration in the culture medium by release from apatite-containing and wollastonite-containing glass ceramics enhanced alkaline phosphatase activity, and promoted osteogenic differentiation in ROS17/2.8 cells.…”

Section: Discussionmentioning

confidence: 99%

Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities

Liu

Zhao

et al. 2012

IJN

View full text Add to dashboard Cite

Introduction:Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects. Methods: Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140°C, a rod-like crystal (n-HA1) with a diameter of 23 ± 5 nm, a length of 47 ± 14 nm, and crystallinity of 85% ± 5% was produced, while at a temperature of 80°C, a rod-like crystal (n-HA2) with a diameter of 16 ± 3 nm, a length of 40 ± 10 nm, and crystallinity of 65% ± 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry. Results: n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response. Conclusion: This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.

show abstract

“…HA has a composition and structure analogous to the bone apatite and shows high bioactivity (Suchanek et al, 2002;Landi et al, 2004 ;). Calcium silicate is used in drug delivery and bone tissue regeneration due to its good biocompatibility, bioactivity, and degradability (Matsuoka et al, 1999;Oyane et al, 2003;Cortes et al, 2004;Jain et al, 2005;Kokubo et al, 2005). Recently, the key point of the present research aims to the synthesis of HA (Rudin et al, 2009) and calcium silicate nanostructures by novel methods.…”

Section: Fabrication Of Calcium-based Inorganic Biodegradable Nanomatmentioning

confidence: 99%

Biomineralization and Biomimetic Synthesis of Biomineral and Nanomaterials

Ma¹,

Sun²

2011

Advances in Biomimetics

View full text Add to dashboard Cite

IntroductionBiominerals and biomaterials with unique microstructure are mainly consisted of organic and inorganic materials, and exhibit excellent biological and mechanical properties. The formation mechanism of biomineral indicated that the organic matrixes have an important influence on the morphology and structure of the inorganic matrix material in the process of biomineralization. However, the biomineralization mechanism research of biomineral is still in the initial stage, many phenomena need to be further explored, such as the effect of organisms on the different morphologies and polymorphs of biominerals, the biomineralization mechanism of the formation process of the biomineral in the existence of organic matter. Biomineralization and biomimetic synthesis of biomineral and nanomaterials have been receiving considerable attention. Biomineralization is the formation process of mineral by organisms. Biomimetic synthesis is simulation of biomineralization, using the mechanism of biomineralization, to achieve biomineral and materials with special structure and function. In a word, biomimetic synthesis is learning from the mineralization of organisms and learning from nature. Therefore, we should understand the concepts, process, and mechanism of biomineralization, which was briefly reviewed in section one. Biomineral including calcium phosphate, hydroxyapatite (HA), calcium silicate, calcium carbonate, and calcium sulfate, are important calcium-based inorganic biodegradable materials and have been widely used in biomedical field. Biomineralization is the mineralization of biomineral. Biomimetic synthesis was first used in the fabrication of biomineral. So it is necessary to provide an overview of the biomimetic synthesis of biomineral. This chapter summarizes our recent endeavors on the biomimetic synthesis of biomineral including HA, calcium silicate, CaCO 3 , BaCO 3 , and SrCO 3 , etc. Biomineral synthesis includes morphology, structure, and function biomineral synthesis. It is well known that the structure determines property and the morphology is the external display of structure. Here, we intend to review recent progress in biomineral synthesis of other nanomaterials.

show abstract

In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Cited by 80 publications

References 33 publications

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities

Biomineralization and Biomimetic Synthesis of Biomineral and Nanomaterials

Contact Info

Product

Resources

About