The microbial adhesive arsenal deciphered by atomic force microscopy

Abstract

Microbes employ a variety of strategies to adhere to abiotic and biotic surfaces, as well as host cells.

Direct contact, dissolution and generation of reactive oxygen species: How to optimize the antibacterial effects of layered double hydroxides

“…In this context, the use of recent AFM-based force spectroscopy modes would be helpful, e.g. single-molecule and single-cell force spectroscopies (SPFS and SCFS, respectively [82,83]. The use of such force spectroscopy approaches would allow not only a qualitative but also a quantitative evaluation of the presented forces of interactions at the singleparticle or single-cell levels.…”

Direct contact, dissolution and generation of reactive oxygen species: How to optimize the antibacterial effects of layered double hydroxides

“…In this context, the use of recent AFM-based force spectroscopy modes would be helpful, e.g. single-molecule and single-cell force spectroscopies (SPFS and SCFS, respectively [87,88]. The use of such force spectroscopy approaches would allow not only a qualitative but also a quantitative evaluation of the presented forces of interactions at the single-particle or single-cell levels.…”

Understanding the role of surface interactions in the antibacterial activity of layered double hydroxide nanoparticles by atomic force microscopy

“…AFM is considered advantageous compared to other highresolution techniques because it allows the analysis of samples in solutions, thus mimicking physiologically relevant conditions. 37 Thanks to the development of the AFM-based force spectroscopy (FS) approaches, the use of AFM is not only limited to being a powerful tool for high resolution imaging of bacterial cells but can also be used to probe bacterial adhesion phenomena. 38,39 In this context, AFM provides a quantitative evaluation of the adhesion interactions between bacteria and Zn-based antimicrobial LDHs which could yield a potential correlation with their antibacterial properties.…”

“…AFM is considered advantageous compared to other high-resolution techniques, because it allows analysis of samples in liquid, thus mimicking physiologically relevant conditions. 37 Thanks to the development of AFM-based force spectroscopy (FS) approaches, AFM was not only limited to be…”