The Mi15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens

Costes, Valérie; Magen, Valérie; Legouffe, Eric; Durand, L; Baldet, Piérre; Rossi, Jean‐François; Klein, Bernard; Brochier, J

doi:10.1016/s0046-8177(99)90160-0

Cited by 81 publications

(58 citation statements)

References 32 publications

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“…Our results indicate that bone marrow Bx sections stained with anti-CD138 antibody provide an excellent assessment of plasma cell load and infiltration and should be used in the management of patients with plasma cell dyscrasias, particularly when there is a low level of bone marrow involvement. Very little data exist on the clinical utility of plasma cell quantitation by CD138 IHC [4,17,18,24]. Further studies correlating the bone marrow plasma cell percentages obtained by CD138 IHC with clinical and laboratory parameters may be warranted.…”

Section: Discussionmentioning

confidence: 99%

“…The use of CD138 (syndecan-1) as an immunohistochemical marker [16][17][18] was a major breakthrough in the detection of plasma cell infiltrates in formalin-fixed, paraffin-embedded material. Syndecan-1 is a transmembrane heparan sulfate-rich proteoglycan that consists of 5 glycoaminoglycans and 1 core protein [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, nonspecific immunoreactivity has been described in bone marrow extracellular milieu, where shed CD138 tends to accumulate in fibrotic stroma [24]. Nevertheless, in the context of hematopoietic cell identification, CD138 is a highly specific and sensitive [28] marker of normal and neoplastic plasma cells [16][17][18] and is expressed by lymphoid tumors with plasmacytic differentiation [17,26].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of bone marrow plasma cell infiltrates in multiple myeloma: the added value of CD138 immunohistochemistry

et al. 2007

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SummaryAssessment of bone marrow involvement by malignant plasma cells is an important element in the diagnosis and follow-up of patients with multiple myeloma and other plasma cell dyscrasias. Microscope-based differential counts of bone marrow aspirates are used as the primary method to evaluate bone marrow plasma cell percentages. However, multiple myeloma is often a focal process, a fact that impacts the accuracy and reliability of the results of bone marrow plasma cell percentages obtained by differential counts of bone marrow aspirate smears. Moreover, the interobserver and intraobserver reproducibility of counting bone marrow plasma cells microscopically has not been adequately tested. CD138 allows excellent assessment of plasma cell numbers and distribution in bone marrow biopsies. We compared estimates of plasma cell percentages in bone marrow aspirates and in hematoxylin-eosin-and CD138-stained bone marrow biopsy sections (CD138 sections) in 79 bone marrows from patients with multiple myeloma. There was a notable discrepancy in bone marrow plasma cell percentages using the different methods of observation. In particular, there was a relatively poor concordance of plasma cell percentage estimation between aspirate smears and CD138 sections. Estimates of plasma cell percentage using CD138 sections demonstrated the highest interobserver concordance. This observation was supported by computer-assisted image analysis. In addition, CD138 expression highlighted patterns of plasma cell infiltration indicative of neoplasia even in the absence of plasmacytosis. We conclude that examination of CD138 sections should be considered for routine use in the estimation of plasma cell load in the bone marrow.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of bone marrow plasma cell infiltrates in multiple myeloma: the added value of CD138 immunohistochemistry

et al. 2007

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“…29 However, after activation and differentiation B cells express high levels of syndecan-1 (CD138). [30][31][32] Elevated expression of HSPG such as syndecan-1 could act as a targets for the pK7 virus in LPSstimulated B cells. 4 Interestingly, targeting adenovirus to the Fc␥II receptor on B cells using monoclonal IgG1 specific for the FLAG epitope resulted in enhanced transduction of the B lymphomas A20 and M12.4.1 and LPS blasts at low multiplicity of infection.…”

Section: Discussionmentioning

confidence: 99%

Efficient transduction of murine B lymphocytes and B lymphoma lines by modified adenoviral vectors: enhancement via targeting to FcR and heparan-containing proteins

Wickham

Keegan

2001

Gene Ther

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Murine lymphocytes are relatively refractory to efficient transfection or retroviral gene transduction. Adenovirus has been used as a vector to transduce a wide variety of cell types. Several advantages of adenoviruses are their ability to transduce non-cycling cells and to transduce the majority of cells in a population. Unfortunately, lymphocytes are not susceptible to infection with conventional adenovirus. Therefore, to express genes efficiently in murine B cells, we tested the ability of genetically modified adenovirus to transduce the ␤-galactosidase gene. We found that adenovirus containing polylysine in the fiber knob was able to efficiently transduce lipopolysaccharide (LPS)-activated splenic B cells and the B lymphoma line M12.4.1; greater than 80% of the cells expressed ␤-galactosidase activity. However, small resting B cells did not express activity unless treated with LPS after infection. This transduction was mediated by inter-

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“…Estes anticorpos pertencem ao "cluster" CD138 (Sanderson et al, 1989;Wijdenes et al, 1996;Costes et al, 1999;Sanderson et al, 2002;Rawstron et al, 2008 (Bernfield et al,1992;Wijdenes et al, 1996;Jourdan et al,1998;Costes et al, 1999) .…”

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confidence: 99%

Anormalidades citogenéticas e sua relação com a proliferação e a apoptose celular em portadores de mieloma múltiplo

Linardi¹

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Ao Prof. Dr. Pedro Enrique Dorlhiac Llacer, meu orientador, por ter me aceitado como aluna e por ter acreditado em meu trabalho. Agradeço por todo apoio e incentivo prestados durante este longo caminho percorrido. À Dra. Gracia Aparecida Martinez, pelo carinho com que me recebeu, pelo incentivo e por ter me ensinado tanto ao longo de todos estes anos de convivência. Obrigada por estar sempre ao meu lado, me apoiando nos momentos mais difíceis.À Dra. Elvira D. Rodrigues Pereira Velloso, cuja participação foi essencial para a realização deste trabalho. Agradeço pelos ensinamentos, pela paciência, pela disponibilidade irrestrita e principalmente, pelo otimismo com que sempre me apoiou.Às amigas Cristina Aiko Kumeda e Aline de Medeiros Leal, para quem não existem palavras que traduzam minha gratidão. Agradeço pela grande ajuda, por estarem sempre disponíveis, pelas intermináveis leituras de FISH (nem sempre nas melhores horas!) e principalmente, por suportarem a minha teimosia.À Dra. Valeria Buccheri, querida amiga, pelo entusiasmo, pelo incentivo à investigação científica e por contribuir de forma tão importante para a minha formação profissional. Agradeço, principalmente, por estar sempre disposta a ajudar.

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The Mi15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens

Cited by 81 publications

References 32 publications

Assessment of bone marrow plasma cell infiltrates in multiple myeloma: the added value of CD138 immunohistochemistry

Assessment of bone marrow plasma cell infiltrates in multiple myeloma: the added value of CD138 immunohistochemistry

Efficient transduction of murine B lymphocytes and B lymphoma lines by modified adenoviral vectors: enhancement via targeting to FcR and heparan-containing proteins

Anormalidades citogenéticas e sua relação com a proliferação e a apoptose celular em portadores de mieloma múltiplo

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