The Method of Intraluminal Perfusion of the Human Small Intestine

Modigliani, R; Rambaud, J.-C.; Bernier, J.J.

doi:10.1159/000197443

Cited by 95 publications

(29 citation statements)

References 28 publications

(54 reference statements)

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“…All solutions contained in addition 5 g/l polyethylene glycol 4000 as a nonabsorbable marker. mation has been found to be quite valid when high infusion rates are used, as is the case in the present study (27).…”

Section: Subjectssupporting

confidence: 78%

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Effect of glutamine on water and sodium absorption in human jejunum at baseline and during PGE₁-induced secretion

Coëffier¹,

Hecketsweiler²,

Hecketsweiler³

et al. 2005

Journal of Applied Physiology

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Coëffier, Moïse, Bernadette Hecketsweiler, Philippe Hecketsweiler, and Pierre Déchelotte. Effect of glutamine on water and sodium absorption in human jejunum at baseline and during PGE1-induced secretion. J Appl Physiol 98: 2163-2168, 2005. First published January 20, 2005 doi:10.1152/japplphysiol.00761.2004.-Glutamine, a major fuel for enterocytes, stimulates water and sodium absorption in animal models of secretory diarrhea, but data in humans are still limited. The aim of this study was to investigate the effect of glutamine on jejunal absorption during hypersecretion in humans. In six healthy adults, the effects of glutamine on jejunal absorption were assessed with a triple-lumen tube on two occasions, at baseline and during PGE1-induced hypersecretion (0.1 g ⅐ kg Ϫ1 ⅐ min Ϫ1 ) in a random order. Isoosmolar solutions containing polyethylene glycol 4000 as nonabsorbable marker were infused in the jejunum at 10 ml/min over 1-h periods: saline (sodium chloride 308 mmol/l), glucosemannitol 45:45 mM, glucose 90 mM, alanine-glucose 45:45 mM, glutamine-glucose 45:45 mM, and glutamine 90 mM. Net absorptive and secretory fluxes were measured at steady state. At baseline, glutamine-and alanine-containing solutions induced a threefold increase of water and sodium absorption (P Ͻ 0.05); 90 mM glutamine stimulated water absorption more than 90 mM glucose (3.6 Ϯ 0.6 vs. 1.9 Ϯ 0.3 ml ⅐ min Ϫ1 ⅐ 30 cm Ϫ1 , P Ͻ 0.05). PGE1-induced hypersecretion was reduced (P Ͻ 0.05) by solutions of alanine-glucose, glutamine-glucose, and glutamine 90 mM (P Ͻ 0.05) and reversed to absorption by alanine-glucose and glutamine-glucose. Glutamine and alanine absorption was nearly complete and was not influenced by PGE1. In conclusion, glutamine stimulates water and electrolyte absorption in human jejunum, even during experimental hypersecretion. In addition to the metabolic effects of glutamine, these results support the evaluation of glutamine-containing solutions for the rehydration and the nutritional support of patients with secretory diarrhea.prostaglandins; secretory diarrhea; amino acids; oral rehydration solution

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Section: Subjectssupporting

confidence: 78%

“…The triple-lumen tube method used in this study is more accurate than the doublelumen tube to measure segmental fluxes (4,27). The intrinsic limitation of the intestinal infusion technique comes from the limited length of intestine under study, which does not allow a definite extrapolation of data on the full length of intestine.…”

Section: Discussionmentioning

confidence: 99%

Effect of glutamine on water and sodium absorption in human jejunum at baseline and during PGE₁-induced secretion

Coëffier¹,

Hecketsweiler²,

Hecketsweiler³

et al. 2005

Journal of Applied Physiology

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show abstract

“…Absorption was determined using the triple lumen perfusion technique [5,6,9]. Perfusion catheters were modified for use in premature infants (see below).…”

Section: Perfusion Techniquementioning

confidence: 99%

“…5%. PEG 4000 concentration was determined as previously described and was used to correct for incomplete recovery [9,10]. Luminal disappearance (i.e.…”

Section: Analysesmentioning

confidence: 99%

In vivo Measurements of Glucose Absorption in Preterm Infants

Shulman¹

1999

Neonatology

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Few data are available regarding the kinetics of glucose absorption in the preterm infant. In order to ascertain the kinetics of glucose absorption in the premature infant and how rate of infusion and concentration affect absorption, jejunal glucose absorption kinetics were measured in 16 preterm infants by infusing 1, 10, and 100 mM glucose solutions in random order. Seventeen infants were perfused with glucose (100 μmol/min) by infusing 67 mM glucose at 1.5 ml/min and 133 mM glucose at 0.75 ml/min to determine the effect of changes in rate of infusion vs. concentration on glucose absorption. K_m and V_max were related to postnatal age. K_m was correlated inversely with the percentage of feedings received as human milk. Antenatal administration of glucocorticoids appeared to increase V_max. The higher infusion rate resulted in greater glucose absorption than the higher concentration glucose solution. Conclusions: The characteristics of glucose absorption in the preterm infant change with age and are affected by diet, glucocorticoids, and the method of infusion. These data have implications for the feeding of preterm infants.

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“…In both studies, oxprenolol absorption was evaluated in an intestinal segment by a perfusion technique using polyethylene glycol 4000 (PEG) as a water soluble, non absorbable marker (Modigliani et al, 1973).…”

Section: Methodsmentioning

confidence: 99%

Jejunal and ileal absorption of oxprenolol in man: influence of nutrients and digestive secretions on jejunal absorption and systemic availability.

Godbillon¹,

Vidon²,

Palma³

et al. 1987

Brit J Clinical Pharma

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and 2INSERM, H6pital Saint-Lazare, 107, rue du Faubourg Saint-Denis, 75010 Paris, France 1 Study I evaluated the absorption of oxprenolol in the ileum, compared to jejunum, in healthy volunteers by an intestinal perfusion technique. Around 80 mg of drug were delivered as a saline solution directly in the small bowel. 2 Samples taken 30 cm distally to the site of perfusion showed that 63% of perfused oxprenolol was absorbed in the jejunum and 48% in the ileum; the differences were significant. 3 The plasma concentration-time profiles were similar for the two perfusions. The AUC and Cmax values of free and conjugated oxprenolol for the jejunal perfusion were significantly lower than those of ileum. They showed large but consistent intersubject variations in the two treatments. 4 Study II investigated, using the same technique, the influence of nutrients and digestive secretions on jejunal absorption and systemic availability of this drug. A saline (in treatments A and B) or a nutrient (in treatment C) solution containing oxprenolol was perfused into the jejunum below a balloon either inflated (A) or deflated (B and C). 5 The disappearance rate of oxprenolol from the jejunum was unaffected by endogenous secretions. The mean amount of drug absorbed along a 30-cm jejunal segment accounted for 52 (A) and 57% (B) of the total amount perfused. The intestinal absorption rate was markedly increased in the presence of nutrients (mean amount absorbed 96% for C). 6 The change in the rate of disappearance from the intestine had no effect on the systemic availability of oxprenolol (mean AUC values 8740, 8250 and 8020 nmol I-1 h for A, B and C, respectively) or its elimination from plasma. 7 The present findings and those obtained previously by intubation and colonoscopic techniques indicate that oxprenolol is not absorbed in the stomach but is absorbed all along the small and large intestines.

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The Method of Intraluminal Perfusion of the Human Small Intestine

Cited by 95 publications

References 28 publications

Effect of glutamine on water and sodium absorption in human jejunum at baseline and during PGE₁-induced secretion

Effect of glutamine on water and sodium absorption in human jejunum at baseline and during PGE₁-induced secretion

In vivo Measurements of Glucose Absorption in Preterm Infants

Jejunal and ileal absorption of oxprenolol in man: influence of nutrients and digestive secretions on jejunal absorption and systemic availability.

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