The method of calibration curves for immunochromatographic express tests. Part 2. immunochromatographic express tests with quantum dots

Determining antibiotic concentration in human blood provides useful pharmacokinetic information. Commonly used methods such as ELISA require a long time to obtain results and thus cannot be applied when information is needed immediately. In this study, a novel antibody-based lateral flow technique was developed for tetracycline detection in human serum. Contrary to tests developed to analyze food samples, the features of work with serum as analyzed probe were studied for the first time here. The application of labeled and unlabeled specific antibodies was compared. For this purpose, specific and anti-species antibodies were labeled with gold nanoparticles and used for antigen–antibody interaction on the membrane surface with observed staining in the test zone. For both schemes, optimal conditions were established to provide the best sensitivity. The developed assay has a limit of visual detection as low as 35 and 11 ng/mL for the direct and indirect labeled antibodies, respectively. The limit of instrumental detection is from 0.4 to 3.5 ng/mL for diluted and undiluted sera. The use of indirect antibody labeling showed a small increase in sensitivity compared to traditional direct antibody labeling. The developed method showed no cross-reactivity with antibiotics of other classes. The method was used to test samples of serum. The results showed high correlation with the data obtained by ELISA (R2 = 0.98968). The assay provides a quick assessment of the amount of antibiotics in the blood and keeps them under control throughout the duration of therapy.

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“…Human serum samples from our earlier investigations [15,26] were used in this study and stored at −20 °C.…”

Section: Methodsmentioning

confidence: 99%

Development of Immunochromatographic Assay for Determination of Tetracycline in Human Serum

et al. 2018

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“…When the calibration curve for the immunochromatographic analysis was plotted in the linear coordinates, it could be described by an exponential model (Equation 1) [36]. …”

Section: Resultsmentioning

confidence: 99%

Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

et al. 2013

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To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.

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“…Предложены анали-тические зависимости для калибровочных кривых ИХТ, работающих в формате сэндвич и формате конкурентного анализа с рефлектометрической [4] и люминесцентной детекцией [5].…”

Section: научные сообщения введениеunclassified

Development of the Quantitative Immunochromatography Tests for Somatic Deasease Markers Detection

Третьяков¹,

Basharova²,

Iarkov³

et al. 2013

Annals RAMS

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The method of calibration curves for immunochromatographic express tests. Part 2. immunochromatographic express tests with quantum dots

Cited by 3 publications

References 5 publications

Development of Immunochromatographic Assay for Determination of Tetracycline in Human Serum

Development of Immunochromatographic Assay for Determination of Tetracycline in Human Serum

Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

Development of the Quantitative Immunochromatography Tests for Somatic Deasease Markers Detection

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