Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

Berlina, Anna N.; Taranova, Nadezhda A.; Zherdev, Anatoly V.; Санков, М Н; Andreev, I. M.; Мартынов, А. И.; Dzantiev, Boris B.

doi:10.1371/journal.pone.0077485

Cited by 27 publications

(20 citation statements)

References 27 publications

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“…In order to establish this new IgE detection method, we focused initially on the most extensive sources of mite allergens in China [34]: Dermatophagoides pteronyssinus (Der-p) and Dermatophagoides farinae (Der-f). First, we labeled CdSe/ZnS QDs with anti-human IgE antibody to quantify the specific IgE reaction to Der-p and Der-f in the serum of patients with AR.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

2020

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The prevalence of allergic rhinitis (AR) is increasing worldwide. However, the current systems used to measure levels of immunoglobulin E (IgE) in sera are associated with several disadvantages that limit their further application. Consequently, there is a need to develop novel highly sensitive strategies that can rapidly detect IgE in a quantitative manner. The development of such systems will significantly enhance our ability to diagnose, treat, and even prevent AR. Herein, we describe our experience of using quantum dot-based lateral flow immunoassay (QD-LFIA), combined with a portable fluorescence immunoassay chip detector (PFICD), to detect serum-specific IgE against Dermatophagoides pteronyssinus (Der-p) and Dermatophagoides farinae (Der-f), two common mite allergens in China. Our data showed that our system could detect serum-specific levels of IgE against Der-p and Der-f as low as 0.093 IU/mL and 0.087 IU/mL, respectively. We also established a standard curve to determine serumspecific IgE concentrations that correlated well with the clinical BioIC microfluidics system. The sensitivity of our assay was 96.7% for Der-p and 95.5% for Der-f, while the specificity was 87.2% for Der-p and 85.3% for Der-f. Collectively, our results demonstrate that QD-LFIA is a reliable system that could be applied to detect serum-specific IgE in accordance with clinical demands. This QD-LFIA strategy can be applied at home, in hospitals, and in pharmacies, with reduced costs and time requirements when compared with existing techniques. In the future, this system could be developed to detect other types of allergens and in different types of samples (for example, whole blood).

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

2020

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“…In a number of works, the use of quantum dots (QDs) was suggested as a means to increase the sensitivity of ICA [23][24][25][26][27]. QDs are semiconductor nanocrystals that have a narrow and symmetric emission spectrum, which depends on their size and composition [28,29]. QDs allow the reduction of the detection limit in ICA, due to their intense fluorescence and a low background signal.…”

Section: Introductionmentioning

confidence: 99%

Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

et al. 2020

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In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than nine times compared with the system using the GNP conjugate.

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“…10 The ability to link QDs with various bioactive molecules without losing the aforementioned characteristics provides wide-eld applications. Quantum dot-based immunochromatographic assays 11,12 or QD-FRET biosensing devices 13 are only two examples of numerous methods using QDs as a signal generating probe. The improved sensitivity, selectivity and multiplexity of QD-based methods enabled us to meet the high demands of the requirements for diagnostic methods for detecting tumours and other clinically important biomolecules.…”

Section: Introductionmentioning

confidence: 99%

An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes

et al. 2017

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Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

Cited by 27 publications

References 27 publications

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes

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