The Methane Fermentations of Acetate and Methanol

Pine, Martin J.; Vishniac, Wolf

doi:10.1128/jb.73.6.736-742.1957

Cited by 44 publications

(20 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acetate was decarboxylated in cell lysates by a reaction in which the methyl group of acetate was reduced to CH4 and the carboxyl group was oxidized to CO2: 14CH3COOH 4 CH4 + CO2. Similar results have been obtained for cells growing on acetate (7,15,20,29). The Km for acetate in the enzyme preparation was 5.12 mM, which compares favorably with that found for cells (27).…”

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri

Baresi¹

1984

J Bacteriol

View full text Add to dashboard Cite

Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH4 and CO2. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cells. Aceticlastic activity was found in the particulate fraction seperate from methylcoenzyme M methylreductase activity, which occurs in the soluble fraction. Pronase treatment eliminated methylcoenzyme M methylreductase activity in lysates and stimulated aceticlastic activity, indicating the aceticlastic activity was not derived from unbroken cells, which are unaffected by proteolytic treatment.for the gift of CH3-S-CoM. I also thank M. Smith, D. Boone, and J. G. Ferry for constructive comments on the manuscript.

show abstract

Section: Discussionsupporting

confidence: 85%

“…Methane is produced from acetate (by decarboxylation), H2-CO2, methanol, formate, and methylamines (4,7,20,27,29). In non-gastrointestinal ecosystems acetate is the principal methanogenic precursor (1,5,28), and its utilization is influenced by the presence of other methanogenic substrates.…”

mentioning

confidence: 99%

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri

Baresi¹

1984

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…the simultaneous utilization of different substrates. Earlier studies showed that, although the normal route for acetate catabolism involves the simultaneous reduction of the C-2 methyl group to methane and the oxidation of the carboxyl to carbon dioxide, the addition of methanol results in the oxidation of the C-2 which provides reducing equivalents for the reduction of the methanol to methane [17]. This result has been verified with several species of methanogen [18-201. Recent studies have suggested that all methanogens utilize the same three carriers at the various oxidation levels between CO2 and CH+ methanofuran (formyl), tetrahydromethanopterin (formyl, hydroxymethyl, and methyl), and coenzyme M (methyl) [ 131.…”

Section: Electron and Carbon Flow Occur Via Common Protein-bound 'Poomentioning

confidence: 90%

A unified scheme for carbon and electron flow coupled to ATP synthesis by substrate‐level phosphorylation in the methanogenic bacteria

Lancaster

1986

FEBS Letters

View full text Add to dashboard Cite

Due primarily to considerations of the nature of the electron transfer reactions which drive ATP synthesis in the methanogenic bacteria, substrate-level phosphorylation (SLP) has been generally ruled out. Recent studies, however, have cast doubt on an obligatory role for a transmembrane ion gradient in coupling. In this paper, a scheme is proposed for SLP coupled to methane formation. The essential features are phosphorylation of bound C, intermediate coupled to intermolecular electron transfer in a multifunctional protein complex containing a collection of bound C, and electron carriers. ATP synthesis is accomplished by subsequent phosphoryl transfer. The scheme is analogous to all known mechanisms of SLP and is compatible with most of the data published to date. Energy metabolism Oxidativephosphorylation MethanogenElectron transport ATP Anaerobiosis

show abstract

“…Thus, acetate and H2/CO 2 appear to be the major in situ substrates for methanogenesis. Methanol and other compounds which supply methyl groups have been reported to serve as substrates for methane in several ecosystems, including the rumen [4][5][6]. The source of such methanol may be anaerobic bacteria which contain pectinolytic enzymes that release methanol as a product of pectin degradation [7].…”

Section: Introductionmentioning

confidence: 99%

Methanol increases methane production in rat fecal homogenates

Levine

1982

FEMS Microbiology Letters

View full text Add to dashboard Cite

The Methane Fermentations of Acetate and Methanol

Cited by 44 publications

References 6 publications

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri

A unified scheme for carbon and electron flow coupled to ATP synthesis by substrate‐level phosphorylation in the methanogenic bacteria

Methanol increases methane production in rat fecal homogenates

Contact Info

Product

Resources

About