The metabolism of hydroxyproline

Adams, Elijah

doi:10.1007/bf01795466

Cited by 15 publications

(18 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subject H. L., who excretes a very large quantity of free 4-hydroxyproline resulting from a blocked first step in 4-hydroxyproline catabolism, does not excrete elevated levels of 3-hydroxyproline. This conclusion is consistent with findings in our laboratory2 that although free 3-hydroxyproline is rapidly oxidized by the intact rat, it does not appear to be converted to a A1l pyrroline product analogous to the first product of oxidation of 4-hydroxyproline or of proline (16).…”

Section: Methodssupporting

confidence: 92%

“…Subject H. L. (Table I) has been known since childhood to excrete abnormally large quantities of free 4-hydroxyproline, attributed to a deficiency of 4-hydroxyproline oxidase, the enzyme catalyzing the first step in 4-hydroxyproline catabolism (16). Earlier studies of the excretion and metabolism of 4-hydroxyproline in this subject have been reported in detail (17)(18)(19), and our values for free and total urinary 4-hydroxyproline are consistent with earlier measurements.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

3-Hydroxyproline content of normal urine.

Adams¹,

Ramaswamy²,

Lamon³

1978

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Values for total 3-hydroxyproline and 4-hydroxyproline were obtained from 24-h urine specimens of 18 healthy human subjects ofboth sexes, whose ages ranged from the first to the sixth decade in age. Urinary 3-hydroxyproline levels, not earlier described to our knowledge, were determined by an isotope-dilution method requiring considerable purification and utilizing the amino acid analyzer for final measurement. 3-Hydroxyproline averaged 3% of the corresponding 4-hydroxyproline in individual urine samples. Like 4-hydroxyproline, 3-hydroxyproline excretion is increased in the second decade, and there is generally good correlation between the two values in individual urines. A hydroxyprolinemic subject excreting greatly elevated 4-hydroxyproline levels did not excrete excessive 3-hydroxyproline, consistent with independent catabolic pathways for the two compounds.3-Hydroxyproline appears to be selectively excreted relative to 4-hydroxyproline when compared with the probable total body content of each amino acid. Possible explanations are: a more rapid turnover of basement membrane collagen than interstitial collagen or, alternatively, relatively greater resistance to the proteolytic cleavage of peptides containing 3-hydroxyproline.

show abstract

Section: Methodssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

3-Hydroxyproline content of normal urine.

Adams¹,

Ramaswamy²,

Lamon³

1978

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…4) which suggests that the values obtained for bone resorption rate are valid. It is doubtful if minor changes in urine hydroxyproline can be considered quantitatively representative of bone breakdown, since normally at least 90% of hydroxyproline is degraded within the body [31] and small variations in its metabolism therefore lead to relatively large fluctuations in its excretion. Bone resorption rate increased in the two patients in whom bone mineralisation rate increased markedly with correction of the acidosis, but was unchanged in the four other cases.…”

Section: Discussionmentioning

confidence: 99%

Effect of Correction of Metabolic Acidosis on Bone Mineralisation Rates in Patients with Renal Osteomalacia

Cochran

Wilkinson

1975

Nephron

View full text Add to dashboard Cite

The role of metabolic acidosis in the genesis of renal osteomalacia was investigated by studying bone mineralisation and resorption rates with a combined isotope and balance technique in six patients, before and after the administration of alkali. Correction of blood pH was achieved in five cases and was associated with a significant rise in the bone mineralisation rates and a significant positive trend in the calcium balances. It is suggested that acidosis contributes to the pathogenesis of osteomalacia in renal failure by slowing skeletal mineralisation, possibly by inhibiting bone alkaline phosphatase.

show abstract

“…13), was initially described (195) as part of an inducible pathway of hydroxyproline degradation in Pseudomonas putida (142,196). The reaction is an obligatory first step in the utilization of hydroxy-[.-proline in Pseudomonas, as inferred from the finding that epirnerase-lacking mutants can grow on allohydroxy-Dproline but not on hydroxy-L-proline (1 97).…”

Section: Hydrox Yproline-2-ep Tmerasementioning

confidence: 99%