The Metabolism of Amino Acids in Developing Rat Brain*

Bayer, Sebastian; McMurray, W. C.

doi:10.1111/j.1471-4159.1967.tb10303.x

Cited by 101 publications

(41 citation statements)

References 34 publications

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“…The maximum increase of the glutamate dehydrogenase activity during the first 1 or 2 weeks of culturing is analogously in agree ment with the fact that the activity of this enzyme in the developing rat brain occurs specially fast during the first 2 weeks of life [4,31]. The specific activity of this enzyme in 2-week-old cultures (10 nmol/min/mg protein) is similar to that in 4-week-old primary cul tures of rat astrocytes observed by RothScliechter et al [50] and that in C-6 glioma cells found by Passonneati et al [48], The g reason for the decline of the activity of the astrocytes [37;Norenberg,pers.…”

Section: ]supporting

confidence: 81%

“…1-2), suggesting the existence of different populations of mitochondria in the cultured astrocytes. The relatively late increase of the glutamate-oxaloacctate aminotransferase is compatible with the observation that the activ ity of this enzyme in the rat3 brain in vivo shows little alteration during the first post natal week [2] and does not reach its maxi mum activity until an age of at least 3 weeks [2,4,47]. The activity of the glutamatcoxaloacetate aminotransferase in 3-week-old cultures is, furthermore, relatively similar to that in the mature mouse brain [51].…”

Section: ]supporting

confidence: 72%

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GFA Content, Glutamate Uptake and Activity of Glutamate Metabolizing Enzymes in Differentiating Mouse Astrocytes in Primary Cultures

Hertz

Bock

Schousboe³

1978

Dev Neurosci

175

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The effects on GFA content, glutamate uptake and activity of glutamate metabolizing enzymes (glutamine synthetase, glutamate dehydrogenase, glutamate-oxaloacetate transferase) by three variables, i.e., length of culturing period, presence or absence of serum, and presence or absence of dibutyryl cyclic AMP (dBcAMP) were studied in primary cultures of astrocytes from newborn mice. During 3 weeks of culturing the content of GFA, the rate of glutamate uptake and the activity of all three enzymes increased but the period of maximum increase differed between the different parameters. The GFA content and the V_max and K_m for glutamate uptake were increased by dBcAMP and decreased by serum withdrawal but the enzyme activities were relatively little affected.

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Section: ]supporting

confidence: 81%

Section: ]supporting

confidence: 72%

GFA Content, Glutamate Uptake and Activity of Glutamate Metabolizing Enzymes in Differentiating Mouse Astrocytes in Primary Cultures

Hertz

Bock

Schousboe³

1978

Dev Neurosci

175

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“…We confirmed herein that the GABA levels are high in the rat spinal cord at very early stages of postnatal development as has already been reported (16)(17)(18)(19). The en dogeneous GABA content in the rat brain reportedly increases after birth; and at about three weeks, the level is twofold higher than that at birth (18).…”

Section: Discussionsupporting

confidence: 91%

Postnatal development of the GABA system in the rat spinal cord.

1982

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Abstract-To assess the postnatal development of the GABA system in the rat spinal cord, GABA levels and GAD activity, as well as specific [3H] muscimol binding, were determined in the dorsal and ventral areas. GABA levels and GAD activities did not vary in parallel from 1 to 8 postnatal days since the former decreased and the latter increased.After 8 days, however, increases in GABA levels and GAD activities were observed in tissues from the dorsal area and decreases were observed in tissues from the ventral area. Specific [3H]muscimol binding was unexpectedly high in both areas at 1 and 8 days but decreased linearly up to 22 days. Our findings show that there are distinct differences in the development of the GABA system in the rat spinal cord and brain.

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“…Like for lactate, with which it is in equilibrium though pyruvate, conflicting observations were reported for alanine concentrations in the developing rat brain (Bayer and McMurray, 1967;Burri et al, 1990;Tkáč et al, 2003). However, both in vivo and in vitro studies in mice demonstrated that cerebral alanine content is higher at birth and decreases during development (Kulak et al, 2010;Yao et al, 1999), reflecting the high protein turnover in the developing brain as result of cell proliferation and tissue expansion.…”

Section: Energy Metabolismmentioning

confidence: 99%