The neurochemical profile quantified by in vivo 1H NMR spectroscopy

Duarte, João M. N.; Lei, Hongxia; Mlynárik, Vladı́mir; Gruetter, Rolf

doi:10.1016/j.neuroimage.2011.12.038

Cited by 198 publications

(261 citation statements)

References 329 publications

(453 reference statements)

Supporting

Mentioning

254

Contrasting

Unclassified

Order By: Relevance

“…IP3 (Berridge, 2009;Berridge et al, 1989). Inositol and its metabolites are present in glia and neurons, including in myelin lipid membranes (Duarte et al, 2012). Increased inositol could reflect: inflammation resulting in activation of astrocytes and microglia in which inositol levels are relatively high; dysregulation of osmotic balance; and/or reduced metabolism of inositol to PIs related to deficient myelination (Duarte et al, 2012;Schmitt et al, 2015).…”

Section: Metabolic and Structural Changes In Cps Micementioning

confidence: 99%

“…Inositol and its metabolites are present in glia and neurons, including in myelin lipid membranes (Duarte et al, 2012). Increased inositol could reflect: inflammation resulting in activation of astrocytes and microglia in which inositol levels are relatively high; dysregulation of osmotic balance; and/or reduced metabolism of inositol to PIs related to deficient myelination (Duarte et al, 2012;Schmitt et al, 2015). As noted above, CPS mice exhibit increased levels of peripheral and central inflammatory factors (Azzinnari et al, 2014;Fuertig et al, 2016) and decreased expression of oligodendrocyte-myelination genes (Azzinnari et al, 2014;Fuertig et al, 2016).…”

Section: Metabolic and Structural Changes In Cps Micementioning

confidence: 99%

See 1 more Smart Citation

Chronic psychosocial stress in mice leads to changes in brain functional connectivity and metabolite levels comparable to human depression

et al. 2016

View full text Add to dashboard Cite

Human depression, for which chronic psychosocial stress is a major risk factor, is characterized by consistent alterations in neurocircuitry. For example, there is increased functional connectivity (FC) within and between regions comprising the default mode network (DMN) including prefrontal cortex and cingulate cortex. Alterations in network FC are associated with specific aspects of psychopathology. In mice, chronic psychosocial stress (CPS) leads to depression-relevant behavior, including increased fear learning, learned helplessness, fatigue and decreased motivation for reward. Using multimodal in vivo magnetic resonance imaging (MRI) and spectroscopy (MRS), we investigated CPS effects on function and structure in the mouse brain under light anesthesia. Mice underwent a baseline MRI/MRS session, followed by 15-day CPS (n=26) or control handling (n=27), and a post-treatment MRI/MRS session. In BOLD fMRI, relative to controls, CPS mice exhibited robust, reproducible increases in FC within 8 of 9 identified cortical networks, including the prefrontal and cingulate cortices that contribute to the "mouse DMN". CPS mice exhibited increases in between-network FC, including amygdala -prefrontal cortex and amygdala -cingulate cortex. MRS identified metabolic alterations in CPS mice as increased inositol levels in amygdala and increased glycerophosphorylcholine levels in prefrontal cortex. Diffusion-weighted MRI detected increased fractional anisotropic values in the cingulum. This study demonstrates that chronic psychosocial stress induces FC states in the mouse brain analogous to those observed in depression, as well as cerebral metabolism and white matter pathway alterations that contribute to understanding of pathological processes. It also demonstrates the importance of brain imaging to the establishment of valid animal models in translational psychiatry. AbstractHuman depression, for which chronic psychosocial stress is a major risk factor, is characterized by consistent alterations in neurocircuitry. For example, there is increased functional connectivity (FC) within and between regions comprising the default mode network (DMN) including prefrontal cortex and cingulate cortex. Alterations in network FC are associated with specific aspects of psychopathology. In mice, chronic psychosocial stress (CPS) leads to depression-relevant behavior, including increased fear learning, learned helplessness, fatigue and decreased motivation for reward.Using multimodal in vivo magnetic resonance imaging (MRI) and spectroscopy (MRS), we investigated CPS effects on function and structure in the mouse brain under light anesthesia. Mice underwent a baseline MRI/MRS session, followed by 15-day CPS (n=26) or control handling (n=27), and a posttreatment MRI/MRS session. In BOLD fMRI, relative to controls, CPS mice exhibited robust, reproducible increases in FC within 8 of 9 identified cortical networks, including the prefrontal and cingulate cortices that contribute to the "mouse DMN". CPS mice exhibited increases in b...

show abstract

Section: Metabolic and Structural Changes In Cps Micementioning

confidence: 99%

Section: Metabolic and Structural Changes In Cps Micementioning

confidence: 99%

Chronic psychosocial stress in mice leads to changes in brain functional connectivity and metabolite levels comparable to human depression

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The similarities and differences between AQSES, which was used in this study, and other quantification methods have been described by Poullet et al [11]. The extensive use of prior knowledge allows the quantification of 20 or more metabolites, at least for high magnetic field strengths and excellent B 0 homogeneity [5,[14][15][16][17][18][19]. However, even under favorable experimental conditions and if correct prior knowledge is used, the separate quantification of some metabolites is difficult.…”

Section: U N C O R R E C T E D P R O O F Introductionmentioning

confidence: 76%

“…However, a separate quantification of Cr and PCr is hampered independent of line width, even for small temperature changes from 37 °C. While the simulations were performed for 7 T, small changes of chemical shifts due to pyrexia or anaesthesia should also be taken into account at higher B 0 to avoid quantification errors, even in case of an excellent separation between the CH 2 signals of Cr and PCr [16][17][18]. Alternatively, the CH 3 and CH 2 signals of Cr and PCr could be modelled as separate singlet signals.…”

Section: Discussionmentioning

confidence: 99%

Temperature dependence of 1H NMR chemical shifts and its influence on estimated metabolite concentrations

Wermter

Mitschke

Bock

et al. 2017

Magn Reson Mater Phy

View full text Add to dashboard Cite

“…Pathological conditions characterized by acute failure of energy metabolism, i.e., ischemic stroke, hypoxia, and traumatic brain injury, exhibit drastic NAA attenuation from within minutes to hours following the specific initiating event. 4,5 This attenuation during the acute stage may initially involve a deterioration of NAA synthesis, acceleration of NAA catabolism, or their combination. These pathologic conditions often lead to a combination of brain tissue necrosis and neuronal loss within several days to a week following the ischemic event unless energy metabolism is resumed within the first several hours.…”

Section: Introductionmentioning

confidence: 99%

N-acetylaspartate Decrease in Acute Stage of Ischemic Stroke:A Perspective from Experimental and Clinical Studies

et al. 2015

View full text Add to dashboard Cite

N-acetylaspartate (NAA) appears in a prominent peak in proton magnetic resonance spectroscopy ( 1 H-MRS) of the brain. Exhibition by NAA of time-dependent attenuation that reflects energy metabolism during the acute stage of cerebral ischemia makes this metabolite a unique biomarker for assessing ischemic stroke. Although magnetic resonance (MR) imaging is a powerful technique for inspecting the pathological changes that occur during ischemic stroke, biomarkers that directly reflect the drastic metabolic changes associated with acute-stage ischemia are strongly warranted for appropriate therapeutic decision-making in daily clinical settings. In this review, we provide a brief overview of NAA metabolism and focus on the use of attenuation in NAA as a means for assessing the pathophysiological changes that occur during the acute stage of ischemic stroke.

show abstract

The neurochemical profile quantified by in vivo 1H NMR spectroscopy

Cited by 198 publications

References 329 publications

Chronic psychosocial stress in mice leads to changes in brain functional connectivity and metabolite levels comparable to human depression

Chronic psychosocial stress in mice leads to changes in brain functional connectivity and metabolite levels comparable to human depression

Temperature dependence of 1H NMR chemical shifts and its influence on estimated metabolite concentrations

N-acetylaspartate Decrease in Acute Stage of Ischemic Stroke:A Perspective from Experimental and Clinical Studies

Contact Info

Product

Resources

About