The metabolic uptake of sulphate ions by skin

The adenosine triphosphate content and lactic acid production of guinea-pig skin after mild heat damage

“…It is apparent that the ATP content of skin is less sensitive to mild heat damage than is the ability to take up oxygen (Lawrence & Ricketts, 1957). Some comparison is possible with the data of Hammer (1973) who reported 38% loss of ATP in human skin deprived of oxygen for 30 min at 37°C.…”

“…The finding that skin heated at 48°C depleted the lactic acid content ofthe serum medium requires further investigation. The data of Lawrence & Ricketts (1957) suggest that about 10% of the normal oxygen uptake may persist after this treatment, and it is possible that oxidation of lactate is occurring. Alternatively if lactic acid production by glycolysis is totally inhibited, some lactic acid may have been lost from the medium by diffusion into the skin.…”

“…The slices were weighed on a torsion balance as they were cut, and collected onto sterile saline. Experiments on the effects of graded thermal damage were made by maintaining the skin samples on saline for 30-min periods at temperatxires ranging from 37 to 48°C (Lawrence & Ricketts, 1957). The adenosine triphosphate (ATP) content of the skin samples was estimated as described by Carney, Hall & Ricketts (1972) using test kits supplied by Boehringer.…”

“…It was therefore of interest to assess the effect ofthe same experimental conditions on the ATP content of skin, since this compound is the primary source of energy for most cells. As the effect of heat on overall respiration measured by oxygen uptake had already been determined by Lawrence & Ricketts (1957) with similar results to those cited above, lactic acid production was measured to estimate the contribution made by glycolysis to ATP production in these circums ances.…”

“…Previous studies on the effect of 30 min exposure of skin explants to temperatures ranging from 37 to 50°C have resulted in sigmoid inhibitive curves showing that temperatures in the region of 43-5°C produce a 50% effect. The effect of heat damage on ^^S04" incorporation by skin was reported by Lawrence & Ricketts (1957), ^^VO^.'" incorporation by Carney, Lawrence and Ricketts (1962) and '*C-proline incorporation by Carney, Lawrence & Ricketts (1965).…”