The adenosine triphosphate content and lactic acid production of guinea-pig skin after mild heat damage

Abstract

The depletion of adenosine triphosphate in skin after mild thermal injury is not large enough to be accounted for by loss of oxidative respiration. Measurement of lactic acid production by skin suggest that glycolysis is less sensitive to heat damage than is oxygen uptake. It is therefore likely that glycolysis makes a large contribution to the relatively high levels of ATP persisting in heat damaged skin.

Comparison of the peripheral mediator background of heat injury- and plantar incision-induced drop of the noxious heat threshold in the rat

“…In accord with this, TNP-ATP was reported to diminish the nocifensive reaction during the late phase of the formalin test in the rat (Jarvis et al 2001) and similar results were obtained in P2X 3 receptor nullmutant mice (Cockayne et al 2000;Souslova et al 2000). It is worth mentioning that high levels of ATP were shown to persist in heatdamaged skin of guinea-pigs (Carney et al 1976). …”

Characterization of the acute temporal changes in excisional murine cutaneous wound inflammation by screening of the wound-edge transcriptome

“…Candidate genes in this category included NADH dehydrogenases, component of the succinate dehydrogenase complex, cytochrome c-1, cytochrome c-oxidase, and ATP synthase. This response may explain wound tissue ATP deficiency that is recognized as a common limiting factor in cutaneous wound healing (7,11).…”

The succinic dehydrogenase and cytochrome c oxidase activities of guinea-pig skin after mild heat damage

“…This suggests that when glucose is provided as the energy source oxygen uptake in heated skin diminishes initially as a result of the failure of a reaction or reactions prior to the respiratory chain. Carney, Hall & Ricketts (1976) found that glycolysis, measured by lactic acid production, was also more resistant than oxygen uptake to mild thermal injury. It is therefore tempting to suggest that the most heat sensitive step in the energy producing processes of skin lies in the Krebs' cycle.…”