The metabolic interface between Pseudomonas syringae and plant cells

Rico, Arantza; McCraw, Sarah; Preston, Gail M.

doi:10.1016/j.mib.2010.12.008

Cited by 41 publications

(45 citation statements)

References 51 publications

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“…Another reason to include the strain in this study was because insufficient in situ physiological data on its initial epiphytic growth phase currently exist. This is in contrast to known metabolic adaptions to leaf interior sites in subsequent infection steps, which have been studied by others (Beattie and Lindow, 1999;Rico and Preston, 2008;Rico et al, 2011). Our analytical rationale was to achieve ample coverage, sensitivity and robustness required to monitor a phylloplane-specific plant metabolome (that is, exometabolome).…”

Section: Introductionmentioning

confidence: 99%

Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves

Ryffel¹,

Helfrich²,

Kiefer³

et al. 2015

The ISME Journal

119

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The phyllosphere, which is defined as the parts of terrestrial plants above the ground, is a large habitat for different microorganisms that show a high extent of adaption to their environment. A number of hypotheses were generated by culture-independent functional genomics studies to explain the competitiveness of specialized bacteria in the phyllosphere. In contrast, in situ data at the metabolome level as a function of bacterial colonization are lacking. Here, we aimed to obtain new insights into the metabolic interplay between host and epiphytes upon colonization of Arabidopsis thaliana leaves in a controlled laboratory setting using environmental metabolomics approaches. Quantitative nuclear magnetic resonance (NMR) and imaging high-resolution mass spectrometry (IMS) methods were used to identify Arabidopsis leaf surface compounds and their possible involvement in the epiphytic lifestyle by relative changes in compound pools. The dominant carbohydrates on the leaf surfaces were sucrose, fructose and glucose. These sugars were significantly and specifically altered after epiphytic leaf colonization by the organoheterotroph Sphingomonas melonis or the phytopathogen Pseudomonas syringae pv. tomato, but only to a minor extent by the methylotroph Methylobacterium extorquens. In addition to carbohydrates, IMS revealed surprising alterations in arginine metabolism and phytoalexin biosynthesis that were dependent on the presence of bacteria, which might reflect the consequences of bacterial activity and the recognition of not only pathogens but also commensals by the plant. These results highlight the power of environmental metabolomics to aid in elucidating the molecular basis underlying plantepiphyte interactions in situ.

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Section: Introductionmentioning

confidence: 99%

Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves

Ryffel¹,

Helfrich²,

Kiefer³

et al. 2015

The ISME Journal

119

View full text Add to dashboard Cite

show abstract

“…Successful infections propagate through multiple phases, the simplest deconstruction being entry (Melotto et al, 2008), suppression of basal defense (Boller and He, 2009), reconfiguration of host metabolism (Rico and Preston, 2008), and persistence in an equilibrium related to a metabolic balance between host and pathogen (Rico et al, 2011). However, it is not sufficient to collate a wealth of literature and infer processes from this.…”

Section: Introductionmentioning

confidence: 99%

Integrated Systems View on Networking by Hormones in Arabidopsis Immunity Reveals Multiple Crosstalk for Cytokinin

Naseem¹,

Philippi²,

et al. 2012

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“…# indicates a significant difference between Psl and BTH + Psl or ARB + Psl at P < 0.05. photosynthesis in Arabidopsis infected with P. syringae (Thilmony et al, 2006;Truman et al, 2006) and inhibition of tomato chloroplast function by P. syringae pv. tomato DC3000 were shown (Rico et al, 2011). In our study, in the control and ARB-treated plants following Psl infection in the directly inoculated leaves (3rd) the chlorophyll levels decreased while in the systemic leaves (5th) they increased.…”

Section: Discussionmentioning

confidence: 45%

“…Their production by Psl has been observed (Olczak-Woltman et al, 2009). P. syringae produces effectors and toxins modifying host metabolism to its benefit, particularly by interfering with chloroplast function, which can result in nitrogen mobilization and suppression of plant defence (Rico et al, 2011;Selvaraj and Fofana, 2012). Truman et al (2006) identified suppression of genes associated with photosynthesis, consistent with the loss of chlorophyll fluorescence, which could have an impact on primary carbon Severe disease symptoms in the form of irregular necrotic areas were visible on Pseudomonas syringae pv.…”

Section: Introductionmentioning

confidence: 95%