Arbutin- and benzotiadiazole-mediated cucumber response to Pseudomonas syringae pv. lachrymans infection in carbohydrate metabolism

Skłodowska, Maria; Naliwajski, Marcin; Wielanek, Marzena; Gajewska, Ewa; Kużniak, Elźbieta

doi:10.1016/j.scienta.2015.06.007

Cited by 5 publications

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References 44 publications

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“…Pathogen infection often leads to changes in the distribution and metabolism of carbohydrates in plants. It is assumed that in the infected source leaves, the biotrophic pathogens’ demand for nutrients, mainly carbohydrates, creates an additional sink, and cell-wall acid invertase is a crucial factor in determining the sink strength in plants with sucrose as the primary translocated sugar [ 46 , 47 , 48 ]. According to Roitsch et al [ 49 ], the induction of cell wall invertase can be interpreted as a source-to-sink conversion of the pathogen-colonized areas.…”

Section: Discussionmentioning

confidence: 99%

Alterations in Primary Carbon Metabolism in Cucumber Infected with Pseudomonas syringae pv lachrymans: Local and Systemic Responses

Kopczewski

Kużniak

Ciereszko

et al. 2022

IJMS

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The reconfiguration of the primary metabolism is essential in plant–pathogen interactions. We compared the local metabolic responses of cucumber leaves inoculated with Pseudomonas syringae pv lachrymans (Psl) with those in non-inoculated systemic leaves, by examining the changes in the nicotinamide adenine dinucleotides pools, the concentration of soluble carbohydrates and activities/gene expression of carbohydrate metabolism-related enzymes, the expression of photosynthesis-related genes, and the tricarboxylic acid cycle-linked metabolite contents and enzyme activities. In the infected leaves, Psl induced a metabolic signature with an altered [NAD(P)H]/[NAD(P)+] ratio; decreased glucose and sucrose contents, along with a changed invertase gene expression; and increased glucose turnover and accumulation of raffinose, trehalose, and myo-inositol. The accumulation of oxaloacetic and malic acids, enhanced activities, and gene expression of fumarase and l-malate dehydrogenase, as well as the increased respiration rate in the infected leaves, indicated that Psl induced the tricarboxylic acid cycle. The changes in gene expression of ribulose-l,5-bis-phosphate carboxylase/oxygenase large unit, phosphoenolpyruvate carboxylase and chloroplast glyceraldehyde-3-phosphate dehydrogenase were compatible with a net photosynthesis decline described earlier. Psl triggered metabolic changes common to the infected and non-infected leaves, the dynamics of which differed quantitatively (e.g., malic acid content and metabolism, glucose-6-phosphate accumulation, and glucose-6-phosphate dehydrogenase activity) and those specifically related to the local or systemic response (e.g., changes in the sugar content and turnover). Therefore, metabolic changes in the systemic leaves may be part of the global effects of local infection on the whole-plant metabolism and also represent a specific acclimation response contributing to balancing growth and defense.

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