Benzothiadiazole and nitrogen source modify the nitrogen metabolism in cucumber infected with Pseudomonas syringae pv. lachrymans

Skłodowska, Maria; Gajewska, Ewa; Naliwajski, Marcin; Wielanek, Marzena; Kużniak, Elźbieta; Chojak-Koźniewska, Joanna

doi:10.1016/j.scienta.2018.10.032

Cited by 3 publications

(4 citation statements)

References 54 publications

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“…The main hosts are cucumbers, tomato, apples, olives, oats, rice, flowers, and more [4]. Angular leaf spot (ALS) is a widespread cucumber disease caused by this phytopathogen, limiting its open-field production [5][6][7]. The symptoms are water-soaked lesions on the leaves, and when they become necrotic contribute to the reduced often offering higher sensitivities thanks to advanced transducer technology [42].…”

Section: Introductionmentioning

confidence: 99%

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Cebula¹,

Żołędowska²,

Dziąbowska³

et al. 2019

Sensors

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The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of Psl were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 103–1.2 × 105 CFU/mL (colony-forming unit) with an R2 coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.

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Section: Introductionmentioning

confidence: 99%

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Cebula¹,

Żołędowska²,

Dziąbowska³

et al. 2019

Sensors

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“…However, opposite effects were presented by Fernández -Crespo (2015). A high proportion of urea in N nutrition resulted in strong disease symptoms in cucumber (Skłodowska et al, 2019). These contrasting results highlight the complexity of N impact on disease severity.…”

Section: Susceptibility Of Host Plants To Ps Is Dependent On Nutrient...mentioning

confidence: 94%

“…For example, Cu sprays reduced bacterial canker in French prune only in addition to biannual application of NPK plus micronutrients (Sayler & Kirkpatrick, 2003). The effectiveness of foliar treatments with benzothiadiazole (BTH) in cucumber was dependent on the N source (different ratios of NO 3 -, NH 4 + , and urea; Skłodowska et al, 2019). Treatments of tomato with PABA were significantly more effective on plants that had received additional NPK fertilizer (Goodwin et al, 2018).…”

Section: Controlling Ps Diseases With Leaf Fertilizers and Foliar Spraysmentioning

confidence: 99%

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Liebig review: The role of mineral nutrients in the development of Pseudomonas syringae diseases—Lessons learned and implications for disease control in woody plants

Soethe,

Hulin,

Balasus

et al. 2024

J. Plant Nutr. Soil Sci.

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BackgroundThe plant pathogen Pseudomonas syringae (Ps) causes diseases in a broad range of hosts including important cash crops from several climate zones. In particular for woody crops, effective and environmentally friendly disease control strategies are not available. Although there is increasing evidence of a link between plant nutritional status and disease susceptibility, optimization of plant nutrition is often neglected in efforts to control Ps diseases. This review brings current knowledge on this topic together with the aim of facilitating the development of treatment recommendations in specific contexts.Literature reviewThe article consists of three parts. First, we compiled data on the impact of mineral nutrients on Ps disease severity in woody and herbaceous species. Next, we discuss how nutrients may be related to plant defense and/or Ps virulence. Last, we consider these findings in the context of woody hosts and give suggestions for future research.ConclusionsWe encourage a research focus on typical nutrient imbalances (deficiencies and surpluses) in specific orchard regions; testing the role of foliar fertilizers in spring (the period of highest infection risk); analyses of the interaction between nutrient supply and the microbiome in the phyllosphere; investigating the interaction between nutrient supply and other control measures, and the impact of nutrient supply on Ps diseases in the presence of other stress factors.

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Antioxidant response of cucumber leaf tissues treated with prohexadione-Ca to infection with Pseudomonas syringae pv. lachrymans

Skłodowska

Mielczarz

Chojak-Koźniewska

et al. 2021

Scientia Horticulturae

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Benzothiadiazole and nitrogen source modify the nitrogen metabolism in cucumber infected with Pseudomonas syringae pv. lachrymans

Cited by 3 publications

References 54 publications

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Liebig review: The role of mineral nutrients in the development of Pseudomonas syringae diseases—Lessons learned and implications for disease control in woody plants

Antioxidant response of cucumber leaf tissues treated with prohexadione-Ca to infection with Pseudomonas syringae pv. lachrymans

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