The metabolic effects in man of bovine growth hormone digested with trypsin

Sonenberg, Martin; Free, Charles A.; Dellacha, Juan M.; Bonadonna, Giovanni; Haymowitz, Asher; Nadler, Allen C.

doi:10.1016/0026-0495(65)90090-9

Cited by 38 publications

(9 citation statements)

References 34 publications

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“…These results seem to be consistent with the previous studies which failed to demonstrate a correlation between serum GH and plasma fasting insulin levels (Luft et al ., 1967; Roelfsema & Frölich, 1985). GH has been shown to have opposing hyperglycaemic and hypoglycaemic activities (Sonenberg et al ., 1965; Swislocki, 1968). In addition plasma concentrations of IGF‐I, which has been demonstrated to have insulin‐like activity (Di Cola et al ., 1997), are elevated in acromegalic patients.…”

Section: Discussionmentioning

confidence: 99%

Impaired β‐cell function in the presence of reduced insulin sensitivity determines glucose tolerance status in acromegalic patients

Kasayama

Otsuki

Takagi

et al. 2000

Clinical Endocrinology

145

105

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Section: Discussionmentioning

confidence: 99%

Impaired β‐cell function in the presence of reduced insulin sensitivity determines glucose tolerance status in acromegalic patients

Kasayama

Otsuki

Takagi

et al. 2000

Clinical Endocrinology

145

105

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“…Although bGH itself has no activity when administered to man, chymotryptic digests (59, 258), tryptic digests (178, 259), or papain digests of bGH (257) all elicited metabolic effects typical of hGH in hypopituitary subjects. The bGH frag ment A II also produced similar effects in hypopituitary children (142).…”

Section: Active Fragments Of Growth Hormone and Chorionic Somatomammomentioning

confidence: 99%

The Regulation of Growth by Endocrines

Daughaday¹,

Herington²,

Phillips³

1975

Annu. Rev. Physiol.

107

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Normal growth is the product of a complex interaction among nutrition, .circulating hormones, and changing tissue responsiveness. Although malnutrition is probably the world's most common cause of poor growth, the greatest advances in our understanding of the growth process have come from studies of the function of endocrine factors in normal and disordered growth. Limitations of space have led us to give greatest attention to growth hormone and somatomedin because of the many recent developments in these areas. We have emphasized clinical research in many areas to take advantage of the increases in knowledge that have come from the application of modern immunoassay techniques to growth problems in man. SOMATOMAMMOTROPINSThe importance of the pituitary growth hormone (GH) in the regulation of growth in most vertebrate species is generally accepted. Earlier studies have been extensively reviewed and will not be discussed here (34, SO, 60, Ill, 132, 153,215,222,274,297). ChemistryMajor advances have recently been made in the elucidation of the primary structure of many of the mammalian growth hormones and of the other somatotropic or lactogenic polypeptide hormones. Excellent reviews of these studies have been pre sented (182,184,195,303).Annu. Rev. Physiol. 1975.37:211-244. Downloaded from www.annualreviews.org Access provided by University of California -San Francisco UCSF on 02/08/15. For personal use only.Quick links to online content Further ANNUAL REVIEWS

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“…The whole digest was not well tolerated by the patients, and it was not further purified or characterized. Sonenberg et al (5) in 1965 described partial digestion of bGH with trypsin; they used soy bean trypsin inhibitor to control the extent of digestion. The purification and characterization of the reaction products have been described in a series of papers (6)(7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and Man

Mills¹,

Reagan²,

Rudman³

et al. 1973

J. Clin. Invest.

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A B S T R A C T As a first step in our study of structurefunction relationships among primate and non-primate growth hormones, human growth hormone (hGH) was subjected to the limited digestive activity of human plasmin. The lyophilized whole digest, containing less than 2% of unchanged hormone, had an average of 2. The digest also caused glucosuria in partially pancreatectomized rats treated with dexamethasone.These metabolic actions of plasmin-digested hGH in the array of animal tests were confirmed by comparable effects elicited in 11 human subjects (nine pituitary-deficient children and adolescents and two nondeficient adults). A single injection of the plasmin digest caused an increase in plasma free fatty acids and a fall in plasma amino acids. Seven daily injections caused positive balances of nitrogen, phosphorous, sodium, and potassium, gain in body weight, and in two of three subjects impairment of glucose tolerance. The potency of the plasmin digest in producing these metabolic effects in man was comparable to that of native hGH.Thus, 2-3 bonds in the hGH molecule can be cleaved by plasmin without impairing the hormone's growth-

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The metabolic effects in man of bovine growth hormone digested with trypsin

Cited by 38 publications

References 34 publications

Impaired β‐cell function in the presence of reduced insulin sensitivity determines glucose tolerance status in acromegalic patients

Impaired β‐cell function in the presence of reduced insulin sensitivity determines glucose tolerance status in acromegalic patients

The Regulation of Growth by Endocrines

Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and Man

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