The membranous labyrinth in baculovirus-infected crustacean cells: possible roles in viral reproduction

Abstract

The origins and morphogenesis of the membranous labyrinth (ML) in Baculov~rus penaei (BP) infected cells of penaeid shrimps (Crustacea:Decapoda) are described. The \IL is usually a highly ordered, complex system of membranes arranged as a labyrinth or in concentric form in the cytoplasm near or against the nuclear envelope. The ML originates from dilated Golgi and endoplasmic reticulum vesicles, and from the outer nuclear envelope. It grows apparently from proliferation of the cellular membranes of these systems, and its developn~ent may be correlated with the stages of BP infection. It is hypothesized that, because of the close parallel and concurrent developnlent of the ML and virus reproduction, and other evidence, the ML is virus-~nduced and -controlled and may play at least 3 roles In the virus reproductive strategy: (1) provides a conduit or transport system for viral precursors from cytoplasm to nucleus; (2) provides ~ncreasc!d membrane surface and volume for increased ATPase activity (related to energy demand for virus reproduction and transport of viral products); and (3) provides a mechanism for cell collapse and release of virus and occlusion bodies at the end of virus reproduction period. Possible experimental methods with which to determine the functional role of the ML are discussed and implications for such a system for nuclear. DNA, viruses are considered.

Pathology and ultrastructure of an intranuclear bacilliform virus (IBV) infecting brown shrimp Crangon crangon (Decapoda: Crangonidae)

“…Infected cells were seen to contain large numbers of mitochondria, showed dilation of the outer nuclear membrane, and often developed an intricate membranous system reminiscent of the membranous labyrinth system described by Couch (1989) in Baculovirus penaei (BP)-infected penaeid shrimps. In the BP model, it is suggested that the labyrinth system may originate from 3 sources (the outer nuclear membrane, the Golgi and the endoplasmic reticulum) and that it can form concentric membrane masses or a highly organized labyrinthian structure.…”

“…In the BP model, it is suggested that the labyrinth system may originate from 3 sources (the outer nuclear membrane, the Golgi and the endoplasmic reticulum) and that it can form concentric membrane masses or a highly organized labyrinthian structure. The role of the labyrinth is to provide structural precursors to the developing virions, and in the case of BP, for the polyhedrin-rich occlusion body (Couch 1989). Modification of cytoplasmic membrane structures has also been noted for other baculovirus infections of shrimps (Lightner et al 1983) and for several viruses from other groups (Couch 1989).…”

“…The role of the labyrinth is to provide structural precursors to the developing virions, and in the case of BP, for the polyhedrin-rich occlusion body (Couch 1989). Modification of cytoplasmic membrane structures has also been noted for other baculovirus infections of shrimps (Lightner et al 1983) and for several viruses from other groups (Couch 1989). Whether the elaborate cytoplasmic membrane system noted in CcBV infection of C. crangon plays a similar role to that suggested for other virus models, or whether it simply represents a pathological symptom of viral infection, remains to be shown.…”

Relationship between BP (Baculovirus penaei) and energy reserves in larval and postlarval Pacific white shrimp Penaeus vannamei

“…Vogt (1992) observed a proliferati.on of smooth endoplasmic reticulum (ER) into concentric membrane whorls indicative of lipid catabolism in HP cells of P monodon infected with the baculovirus MBV He reported that HP cells in the advanced stages of infection lacked lipid reserves. Couch (1989) observed the formation of a membranous labyrinth (ML) from ER vesicles in BP-infected P. duorarum during the early stages of viral infection. He observed that the ML is closely associated with viral repliHours Post-Exoosure .…”

Development of a PCR procedure for the detection of Baculovirus penaei in shrimp

“…Histopathologic damage 'E-mail. sywang@whale st usm edu occurs when hepatopancreatic and midgut epithelia1 cells are destroyed during occlusion body formation and subsequent eruption from host cells (Couch 1981(Couch , 1989.…”