The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol

Nikolaki, Antigoni; Papadioti, Anastasia; Arvaniti, Katerina; Kassotaki, Eleni; Langer, Julian D.; Tsiotis, Georgios

doi:10.1016/j.euprot.2014.04.003

Cited by 2 publications

(4 citation statements)

References 43 publications

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“…phDV1 can use both polyphenols and sugars as carbon sources, with a slight preference for polyphenolic compounds. The strain has been reported to have the ability to degrade aromatic compounds [ 9 , 29 , 37 , 50 ] and can utilize glucose as an energy source [ 51 ]. The delayed use of carbohydrates as a carbon source may be due to their slower transport into the cell compared to hydrophobic aromatic compounds.…”

Section: Resultsmentioning

confidence: 99%

“…The delayed use of carbohydrates as a carbon source may be due to their slower transport into the cell compared to hydrophobic aromatic compounds. utilize glucose as an energy source [51]. The delayed use of carbohydrates as a carbon source may be due to their slower transport into the cell compared to hydrophobic aromatic compounds.…”

Section: Biodegradation Of Sugars and Polyphenolsmentioning

confidence: 99%

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Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

Drakonaki,

Mathioudaki,

Geladas

et al. 2023

Microorganisms

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Pseudomonas sp. phDV1 is a polyhydroxyalkanoate (PHA) producer. The presence of the endogenous PHA depolymerase (phaZ) responsible for the degradation of the intracellular PHA is one of the main shortages in the bacterial production of PHA. Further, the production of PHA can be affected by the regulatory protein phaR, which is important in accumulating different PHA-associated proteins. PHA depolymerase phaZ and phaR knockout mutants of Pseudomonas sp. phDV1 were successfully constructed. We investigate the PHA production from 4.25 mM phenol and grape pomace of the mutants and the wild type. The production was screened by fluorescence microscopy, and the PHA production was quantified by HPLC chromatography. The PHA is composed of Polydroxybutyrate (PHB), as confirmed by 1H-nuclear magnetic resonance analysis. The wildtype strain produces approximately 280 μg PHB after 48 h in grape pomace, while the phaZ knockout mutant produces 310 μg PHB after 72 h in the presence of phenol per gram of cells, respectively. The ability of the phaZ mutant to synthesize high levels of PHB in the presence of monocyclic aromatic compounds may open the possibility of reducing the costs of industrial PHB production.

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Section: Resultsmentioning

confidence: 99%

Section: Biodegradation Of Sugars and Polyphenolsmentioning

confidence: 99%

Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

Drakonaki,

Mathioudaki,

Geladas

et al. 2023

Microorganisms

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“…It was suggested that OmpA's main role is to maintain the cell wall integrity. 10 OprD is a specific porin that binds several antibiotics such as imipenem and related zwitterionic carbapenems as well as basic amino acids. 11 In P. aeruginosa, the OprD family includes 19members, which have a similarity of 46%−57% at the amino acid level.…”

Section: ■ Introductionmentioning

confidence: 99%

“…hDV1 was grown in media containing glucose or phenol, OmpA was found in the bacterial membrane. It was suggested that OmpA’s main role is to maintain the cell wall integrity …”

Section: Introductionmentioning

confidence: 99%

Proteomic Assessment of the Expression of Genes Related to Toluene Catabolism and Porin Synthesis in Pseudomonas stutzeri ST-9

et al. 2017

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The organization and expression of Pseudomonas stutzeri ST-9 genes related to toluene catabolism and porin synthesis was investigated. Toluene-degrading genes were found to be localized in the chromosome close to a phage-type integrase. A regulatory gene and 21 genes related to an aromatics degradation pathway are organized as a putative operon. These proteins are upregulated in the presence of toluene. Fourteen outer membrane proteins were identified as porins in the ST-9 genome. The identified porins showed that the main detected porins are related to the OmpA and OprD superfamilies. The percentage of porins in the outer membrane protein fraction, as determined by mass spectrometry, was 73% and 54% when the cells were cultured with toluene and with glucose, respectively. Upregulation of OmpA and downregulation of OprD occurred in the presence of toluene. A porin fraction (90% OprD) from both cultures was isolated and examined as a toluene uptake system using the liposome-swelling assay. Liposomes were prepared with the porin fraction from a culture that was grown on toluene (T-proteoliposome) or glucose (G-proteoliposome). There was no significant difference in the permeability rate of the different solutes through the T-proteoliposome and the G-proteoliposome.

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The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol

Cited by 2 publications

References 43 publications

Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

Proteomic Assessment of the Expression of Genes Related to Toluene Catabolism and Porin Synthesis in Pseudomonas stutzeri ST-9

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