The mechanisms of action of Tianhua<sup>™</sup>on antitumor activity in lung cancer cells

Li, Chien-Te; Lin, Ching-Hsiung; Kao, Te-Yu; Wu, Ming-Fang; Yeh, Chin-Shui; Yeh, Kun‐Tu; Ko, Jiunn‐Liang

doi:10.3109/13880201003789432

Cited by 22 publications

(19 citation statements)

References 19 publications

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“…The results revealed the anti-leukemia and anti-lymphoma potential of TCS [59]. TCS also manifested antitumor activity in lung cancer cells in vitro and in vivo [60]. Some N-terminal peptides (#1-15, and 16-30) of TCS also manifested antitumor activity in both in vitro and in vivo studies [61].…”

Section: B the Antitumor Activity Of Tcsmentioning

confidence: 99%

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Fang¹,

Ng²,

Shaw³

et al. 2011

CMC

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Ribosome inactivating proteins (RIPs) are toxic RNA N-glycosidases that cleave an adenine-ribose glycosidic bond at position adenine(4324) with the conserved ricin/α-sarcin loop in the eukaryotic 28S ribosomal RNA. RIPs have captured the attention of botanists, biochemists, and drug discoverers, due to their diverse potent defensive activities, and inter alia, their antitumor and anti-HIV activities. Out of the 145 families of plants, Trichosanthes ranks among the top 5 genera with a good potential of use for discovery of anticancer drugs. Trichosanthin (TCS) is a famous type I RIP purified from T. kirilowii that has been known for around 30 years. Based on the results of voluminous in vitro and in vivo investigations, TCS is considered a good candidate for the treatment of HIV/AIDS and neoplasms. Here we integrate recent progress of the research on the different medicinal activities of TCS. In addition to TCS, other promising RIPs from the same species (such as TAP29 and trichoanguin), and from the same genus Trichosanthes are included. This review presents a brief panorama of the studies on Trichosanthes RIPs. Regarding the debilitating nature of AIDS and different tumors, further understanding of these multifunctional proteins is worthwhile since it may help to open a novel therapeutic window for these stubborn diseases.

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Section: B the Antitumor Activity Of Tcsmentioning

confidence: 99%

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Fang¹,

Ng²,

Shaw³

et al. 2011

CMC

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“…Third, the apoptosis-inducing activity of TCS applies to mouse NIH 3T3 embryonic fibroblasts by the activation of caspase-8 and caspase-3 involved pathways [18]. Fourth, TCS induced apoptosis in human lung cancer cells by G1 phase arrest, anti-telomerase effects, and inhibition of cell migration and metastasis [19], also previously observed in murine models [20]. Furthermore, TCS elicited caspase-3-mediated apoptosis in human leukemia HL-60 and K562 cells [21], [22].…”

Section: Introductionmentioning

confidence: 99%

Trichosanthin Inhibits Breast Cancer Cell Proliferation in Both Cell Lines and Nude Mice by Promotion of Apoptosis

Fang

Zhang

et al. 2012

PLoS ONE

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Breast cancer ranks as a common and severe neoplasia in women with increasing incidence as well as high risk of metastasis and relapse. Translational and laboratory-based clinical investigations of new/novel drugs are in progress. Medicinal plants are rich sources of biologically active natural products for drug development. The 27-kDa trichosanthin (TCS) is a ribosome inactivating protein purified from tubers of the Chinese herbal plant Trichosanthes kirilowii Maximowicz (common name Tian Hua Fen). In this study, we extended the potential medicinal applications of TCS from HIV, ferticide, hydatidiform moles, invasive moles, to breast cancer. We found that TCS manifested anti-proliferative and apoptosis-inducing activities in both estrogen-dependent human MCF-7 cells and estrogen-independent MDA-MB-231 cells. Flow cytometric analysis disclosed that TCS induced cell cycle arrest. Further studies revealed that TCS-induced tumor cell apoptosis was attributed to activation of both caspase-8 and caspase-9 regulated pathways. The subsequent events including caspase-3 activation, and increased PARP cleavage. With regard to cell morphology, stereotypical apoptotic features were observed. Moreover, in comparison with control, TCS- treated nude mice bearing MDA-MB-231 xenograft tumors exhibited significantly reduced tumor volume and tumor weight, due to the potent effect of TCS on tumor cell apoptosis as determined by the increase of caspase-3 activation, PARP cleavage, and DNA fragmentation using immunohistochemistry. Considering the clinical efficacy and relative safety of TCS on other human diseases, this work opens up new therapeutic avenues for patients with estrogen-dependent and/or estrogen-independent breast cancers.

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“…T. kirilowii has been reported to have an antitumor effect through experiments using A549 lung cancer cells [7]. P. grandiflorus has been reported to show antimetastatic activities by inhibiting the invasion of cancer cells through a basement membrane.…”

Section: Discussionmentioning

confidence: 99%

A Case of Metastatic Bladder Cancer in Both Lungs Treated with Korean Medicine Therapy Alone

et al. 2014

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This case report is aimed to investigate the effects of Korean medicine therapy (KMT) including oral herbal medicine and herb nebulizer therapy in treating metastatic bladder cancer in the lungs. A 74-year-old man was diagnosed with metastatic bladder cancer in both lungs in August 2013. He refused any chemotherapy and was admitted to our hospital in a much progressed state on January 11, 2014. Since then, he was treated with KMT until May 17, 2014. The main oral herbal medicines were Hyunamdan made of heat-processed ginseng, Hangamdan S made of Cordyceps militaris, Panax ginseng radix, Commiphora myrrha, calculus bovis, margarita, Boswellia carteri, Panax notoginseng radix and Cremastra appendiculata tuber, and nebulizer therapy with Soram nebulizer solution made of wild ginseng and Cordyceps sinensis distillate. Their effect was evaluated considering the change of the main symptoms and using serial chest X-ray. The size and number of multiple metastatic nodules in both lungs were markedly decreased and the symptoms had disappeared. These results suggest that KMT can be an effective method to treat metastatic bladder cancer in the lungs.

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The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Cited by 22 publications

References 19 publications

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Trichosanthin Inhibits Breast Cancer Cell Proliferation in Both Cell Lines and Nude Mice by Promotion of Apoptosis

A Case of Metastatic Bladder Cancer in Both Lungs Treated with Korean Medicine Therapy Alone

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The mechanisms of action of Tianhua™on antitumor activity in lung cancer cells

Cited by 22 publications

References 19 publications

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Recent Progress in Medicinal Investigations on Trichosanthin and other Ribosome Inactivating Proteins from the Plant Genus Trichosanthes

Trichosanthin Inhibits Breast Cancer Cell Proliferation in Both Cell Lines and Nude Mice by Promotion of Apoptosis

A Case of Metastatic Bladder Cancer in Both Lungs Treated with Korean Medicine Therapy Alone

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The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells