The mechanisms of action of carvacrol and its synergism with nisin against Listeria monocytogenes on sliced bologna sausage

Churklam, Wasinee; Chaturongakul, Soraya; Ngamwongsatit, Bhunika; Aunpad, Ratchaneewan

doi:10.1016/j.foodcont.2019.106864

Cited by 106 publications

(60 citation statements)

References 37 publications

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“…In fact, phenolic compounds such carvacrol (major molecule of AIEO) has shown important antibacterial effects with several mechanisms. Indeed, this compound alters cell membrane, inhibits respiratory chain and attenuates genes expression involved in bacterial resistance 32 in several positive and negative strains 53,54 . Moreover, carvacrol can also inhibit the bacterial communication by interfering quorum sensing signalling pathways, which are involved on the establishment of biofilm formation and bacterial resistance to antibiotics 55 …”

Section: Resultsmentioning

confidence: 99%

Pharmacological investigation ofAjuga ivaessential oils collected at three phenological stages

Bouyahya

Omari

Belmehdi

et al. 2020

Flavour & Fragrance J

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Ajuga iva (A iva) is a medicinal plant used mainly in traditional medicine. This study was aimed to identify the volatile compounds of A iva essential oils (AIEO) at three developmental periods, and to evaluate their pharmacological properties. Chemical volatile compounds were identified by GC/MS analysis. Twenty‐eight volatile components were identified, belonging mainly to oxygenated monoterpenes and hydrocarbons monoterpenes. Carvacrol, octadecane, methyl chavicol were the major volatile components of AIEO at the three phenological stages. FRAP, DPPH and ABTS assays were adopted to estimate the antioxidant effects. AIEO at the vegetative stage exhibited important antioxidant effects with IC50 = 52.43 μg/mL (DPPH), IC50 = 115.22 μg/mL (FRAP), and, IC50 = 143.40 μg/mL (ABTS). The antidiabetic activity was evaluated in vitro by the inhibition of α‐amylase and α‐glucosidase. AIEO at the vegetative stage was the most active as an enzyme inhibitor of α‐amylase (IC50 = 101.727 μg/mL) and α‐glucosidase (IC50 = 86.93 μg/mL). The dermatoprotective effect was investigated by the in vitro inhibition of tyrosinase and elastase activities. AIEO at the vegetative stage showed the lowest IC50 inhibition values against tyrosinase (IC50 = 87.49 μg/mL) and elastase (IC50 = 192.21 μg/mL). Antifungal properties of AIEO were investigated on Trichophyton violaceum, T tonsurans and T mentagrophytes. The three EOs of A iva exhibited important inhibitions of the tested dermatophytes. The findings of this work showed that phenological stages affect significantly the volatile compounds of A iva, and these fluctuations influence mainly the biological properties. Moreover, further investigations concerning in vivo pharmacodynamic and pharmacokinetic investigations as well as clinical trials of these identified compounds could develop antidiabetic, dermatoprotective and anti‐dermatophyte drugs.

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Section: Resultsmentioning

confidence: 99%

Pharmacological investigation ofAjuga ivaessential oils collected at three phenological stages

Bouyahya

Omari

Belmehdi

et al. 2020

Flavour & Fragrance J

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“…Nisin (E234) is one of the most examined bacteriocins used as an inhibitory agent of potentially pathogenic bacteria that proliferate in the food industry [1][2][3]. Bacteriocins are relatively small antimicrobial peptides ribosomally synthesized by most known bacteria, e.g., Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria [4].…”

Section: Introductionmentioning

confidence: 99%

Nisin as a Novel Feed Additive: The Effects on Gut Microbial Modulation and Activity, Histological Parameters, and Growth Performance of Broiler Chickens

Kierończyk

Rawski

Mikołajczak

et al. 2020

Animals

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Two independent experiments were performed to evaluate the effect of nisin alone or with monensin on gut microbiota, gut microbial activities, and histomorphology (exp 1) and the effect of nisin application in a dose‒response manner on the growth performance of broiler chickens (exp 2). A total of 900 one-day-old female Ross 308 chicks (400, exp 1; 500, exp 2) were randomly distributed to four groups (exp 1; 10 replicate pens per treatment with 10 birds each), i.e., NA, no additives; MON, monensin (100 ppm); NIS, nisin (2700 IU/kg diet); and MON + NIS, a mixture of monensin (100 ppm) and nisin (2700 IU/kg diet); or 5 treatments (exp 2), NA, no additives; NIS100, nisin (100 IU/kg diet); NIS200, nisin (200 IU/kg diet); NIS400, nisin (400 IU/kg diet); and NIS800, nisin (800 IU/kg diet). Nisin supplementation positively affected the microbiota of the gut by reducing potentially pathogenic bacterial populations in the jejunum and ceca. The bacterial fermentation in the jejunum was significantly lowered by nisin addition. The addition of nisin from 100 IU to 800 IU decreased the FCR value over the entire experimental period. According to the results, nisin can be considered a natural dietary supplement for broiler chickens.

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“…Previous studies have shown that the antimicrobial concentrations needed to control LM are different when tested on broth systems compared to testing directly in the food matrices (26). Since food matrices are compositionally more complex, compared to laboratory media systems, often increased doses of antimicrobials are required to control bacterial growth (8). For example, 200 ppm LAG (maximum limit permitted on food) extended the lag phase of LM in BHI.…”

Section:  Response Surface Methodology Was Used To Analyze Antimicromentioning

confidence: 99%

“…Furthermore, the bactericidal activity of Ni was measured in vitro and on RTE bologna sausage (8). The minimum inhibitory concentration of Ni (25 ppm) was applied directly on the sausage.…”

Section:  Response Surface Methodology Was Used To Analyze Antimicromentioning

confidence: 99%

Enabling Cost-Effective Screening for Antimicrobials against Listeria monocytogenes in Ham

Resendiz-Moctezuma

Rezac

Miller

et al. 2021

Journal of Food Protection

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Ready-to-eat (RTE) meat products, such as deli ham, can support the growth of Listeria monocytogenes (LM) which can cause severe illness in immunocompromised individuals. The objectives of this study were to validate a miniature ham model (MHM) against the ham slice method and screen antimicrobial combinations to control LM on ham using response surface methodology (RSM) as a time- and cost-effective high-throughput screening tool. The effect of nisin (Ni), potassium lactate sodium acetate (PLSDA), lauric arginate (LAG), lytic bacteriophage (P100), and Ɛ-polylysine (EPL) added alone, or in combination, was determined on the MHM over 12 days of storage. Results showed the MHM accurately mimics the ham slice method since no statistical differences were found (p=0.526) in the change of LM cell counts in MHM and slice counts after 12 days of storage at 4°C for treated and untreated hams. The MHM was then used to screen antimicrobial combinations using an on-face design and three center points in a central composite design. The RSM was tested using a cocktail of five LM strains isolated from foodborne disease outbreaks. Three levels of the above mentioned antimicrobials were used in combination for a total of 28 runs performed in triplicate. The change of LM cell counts were determined after 12 days of storage at 4°C. All tested antimicrobials were effective on reducing LM cell counts on ham when added alone. A significant antagonistic interaction (p=0.002) was identified by the RSM between LAG and P100, where this antimicrobial combination caused a 2.2 logCFU/g change of LM cell counts after 12 days of storage. Two interactions, between Ni and EPL (p=0.058), and Ni and P100 (p=0.068), showed possible synergistic effects against LM on the MHM. Other interactions were clearly non-significant, suggesting additive effects. In future work, the developed MHM in combination with RSM can be used as a high-throughput method to analyze novel antimicrobial treatments against LM

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The mechanisms of action of carvacrol and its synergism with nisin against Listeria monocytogenes on sliced bologna sausage

Cited by 106 publications

References 37 publications

Pharmacological investigation ofAjuga ivaessential oils collected at three phenological stages

Pharmacological investigation ofAjuga ivaessential oils collected at three phenological stages

Nisin as a Novel Feed Additive: The Effects on Gut Microbial Modulation and Activity, Histological Parameters, and Growth Performance of Broiler Chickens

Enabling Cost-Effective Screening for Antimicrobials against Listeria monocytogenes in Ham

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