The mechanism of resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in a johnsongrass biotype

Bradley, Kevin W.; Wu, Jun; Hatzios, Kriton K.; Hagood, Edward S.

doi:10.1614/0043-1745(2001)049[0477:tmorta]2.0.co;2

Cited by 50 publications

(38 citation statements)

References 40 publications

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“…However, it showed no evidence of resistance to sethoxydim and no evidence of possessing an insensitive ACCase 8. It is possible that other mechanisms of resistance occur, and resistance to sethoxydim conferred by an overproduction of ACCase in resistant Sorghum halepense (L) (johnsongrass) has been documented 23…”

Section: Discussionmentioning

confidence: 99%

Characterisation of target‐site resistance to ACCase‐inhibiting herbicides in the weed Alopecurus myosuroides (black‐grass)

Moss

Cocker

Brown

et al. 2003

Pest Management Science

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Resistance to aryloxyphenoxypropionate (AOPP), cyclohexanedione (CHD) and phenylurea herbicides was determined in UK populations of Alopecurus myosuroides Huds. Two populations (Oxford AA1, Notts. A1) were highly resistant (Resistance indices 13-->1000) to the AOPP and CHD herbicides fenoxaprop, diclofop, fluazifop-P and sethoxydim, but only marginally resistant to the phenylurea, chlorotoluron. Analyses of acetyl coenzyme A carboxylase (ACCase) activity showed that an insensitive ACCase conferred resistance to all the AOPP/CHD herbicides investigated. Another population, Oxford S1, showed no resistance to sethoxydim at the population level, but contained a small proportion of plants (<10%) with an insensitive ACCase. Genetic studies on the Notts A1 and Oxford S1 populations demonstrated that target site resistance conferred by an insensitive ACCase is monogenic, nuclearly inherited with the resistant allele showing complete dominance. Investigations of the molecular basis of resistance in the Notts A1 population showed that sethoxydim resistance in A myosuroides was associated with the substitution of an isoleucine in susceptible with a leucine in resistant plants, which has also been found in three other resistant grass-weed species (Setaria viridis (L) Beauv, Avena fatua L, Lolium rigidum Gaud).

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Section: Discussionmentioning

confidence: 99%

Characterisation of target‐site resistance to ACCase‐inhibiting herbicides in the weed Alopecurus myosuroides (black‐grass)

Moss

Cocker

Brown

et al. 2003

Pest Management Science

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“…Overproduction of the normal susceptible ACCase has only been reported as a mechanism of resistance in corn (Zea mays L.) cell lines and in a biotype of johnsongrass (Sorghum halepense) resistant to AOPP and CHD herbicides (Bradley et al 2001;Parker et al 1990). …”

Section: Accase Assaysmentioning

confidence: 99%

Basis of selectivity of cyhalofop-butyl in Oryza sativa L.

Ruiz-Santaella

Heredia

Prado

2005

Planta

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Cyhalofop-butyl (CB), 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoic acid, butyl ester (R), is an aryloxyphenoxypropionate (AOPP) herbicide for postemergence use in rice to control grasses, mainly Echinochloa spp. Similar to other AOPP and cyclohexanedione herbicides, the site of action of CB is acetyl-coenzyme A carboxylase (ACCase), an enzyme in fatty acid biosynthesis. The mechanisms involved in the selectivity of CB in rice (Oryza sativa L.)-absorption, translocation, metabolism, and ACCase susceptibility-were studied. Studies of in vitro inhibition of ACCase in E. oryzoides and O. sativa L. species discounted any differential active site sensitivity as the basis of tolerance to CB. The O. sativa L. cuticle was uniformly covered by waxes, with predominantly unshaped large waxes randomly distributed, obtaining absorption values of under 30%, 24 h after application (HAA). The E. oryzoides cuticle formed a non-uniform covered reticule, with less wax density and areas lacking in waxes reaching maximum values of absorption rising to 73%, 24 HAA. Translocation studies revealed no significant differences, either between species, or between times, remaining in the treated leaf. There was a good correlation between the rate of metabolism and plant tolerance. Plant metabolism studies demonstrated that tolerant rice inactivated the esterases producing a lack of functionality thus reducing the conversion of CB to cyhalofop acid, which is the active form of the herbicide. Moreover, it increased the metabolism of the herbicide forming non toxic metabolites much faster than E. oryzoides. It was concluded that the basis of rice tolerance to CB was a lack of esterase functionality, a reduced absorption through the cuticle and an increase in cyhalofop acid metabolism.

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“…populations with resistance to ACCase lierbicide have been reported and eight were described as Johnsongrass \S. The resistance mechanisms were reported as overproduction of ACCase (Bradley et al, 2001), target site resistance that provides cross resistance (Burke et al, 2006a), and target site resistance that provides very little cross resistance (Obermeier, 1998;Smeda et al, 1997). ] (Heap, 2010).…”

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confidence: 99%

Genetic Resistance to Acetyl‐Coenzyme A Carboxylase‐Inhibiting Herbicides in Grain Sorghum

et al. 2012

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Weed management is one of the most important considerations impacting sorghum [Sorghum bicolor (L.) Moench] production in the United States today. The objectives of this study were to evaluate the level of resistance, type of inheritance, and causal mutation of wild sorghum accessions that are resistant to acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides compared to susceptible grain sorghum seed parents. Acetyl-coenzyme A carboxylaseinhibiting herbicides used in this study were fluazifop-P and quizalofop-P from the aryloxyphenoxypropionate (APP) family and clethodim and sethoxydim from the cyclohexanedione (CHD) family. Dose-response studies indicated the level of resistance was very high for APP herbicides but low to nonexistent for CHD herbicides. Estimates of the resistance factors to APP herbicides based on herbicide rate required for 50% growth reduction (GR^Q) were 54 to 64 for homozygous plants and 9 to 19 for heterozygous plants. Resistance to CHD herbicides was very low with resistance factors ranging from one to about five. Genetic segregation studies indicated a single gene is the cause of resistance to APP herbicides, Polymerase chain reaction (PCR) amplicons from the carboxyl transferase (CT) domain of the chloroplast directed ACCase gene were sequenced and a single point mutation that results in a cysteine replacing tryptophan (conversion from tryptophan at amino acid position 2027 to cysteine [Trp-2027-Cys]) was discovered. Given the high level of herbicide resistance and simple inheritance, these sources of APP resistance may provide useful herbicide resistance traits for use in sorghum.

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The mechanism of resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in a johnsongrass biotype

Cited by 50 publications

References 40 publications

Characterisation of target‐site resistance to ACCase‐inhibiting herbicides in the weed Alopecurus myosuroides (black‐grass)

Characterisation of target‐site resistance to ACCase‐inhibiting herbicides in the weed Alopecurus myosuroides (black‐grass)

Basis of selectivity of cyhalofop-butyl in Oryza sativa L.

Genetic Resistance to Acetyl‐Coenzyme A Carboxylase‐Inhibiting Herbicides in Grain Sorghum

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