The Mechanism of Hemolysis in Paroxysmal Cold Hemoglobinuria. Ii. Observations on the Behavior and Nature of the Antibody 1

Paroxysmal Cold Hemoglobinuria in a Patient with Klebsiella pneumonia

“…In our patient the direct antiglobulin test remained positive with antiblobulin re agents containing anti-complement compo nents. Although the D-L antibody is known to be a biphasic hemolysin, it may exhibit agglutinating ability at a lower temperature [14], as was exhibited in the serum and red cell eluates of our patient (table II).…”

The Mechanism of Hemolysis in Paroxysmal Cold Hemoglobinuria. I. The Role of Complement and Its Components in the Donath-Landsteiner Reaction 12

“…In contrast, the immunological character of paroxysmal cold hemoglobinuria is unique in that it requires complement for antibody fixation as well as for subsequent hemolysis. Characterization of the PCH antibody has not helped to explain this phenomenon, since the hemolysin is a gammaglobulin of the pseudoglobulin type (25) and thus falls in the same class as other antibodies.…”

The Nature and Interactions of the Properdin System

“…Perhaps another analogy again exists in the interaction of erythrocytes with cold or "incomplete" antibodies. In this reaction serum factors resembling complement, are required for the combination of the antibody with the erythrocyte ("cold phase") and for the hemolysis of the cell ("warm phase") 13,14 The dual and different requirements for the interaction of properdin could be" considered biologically economic. The bactericidal activity of the properdin system which appears to require all four components of complement results in bacteriolysis.…”