The measurement of plasma digoxin concentration: A comparison of two methods

Lader, S; Bye, Alan; Marsden, Philip D.

doi:10.1007/bf00560891

Cited by 16 publications

(1 citation statement)

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“…Most radioimmunoassays for digoxin developed to date, whether using 3H-digoxin (Smith, Butler, and Haber, 1969;Chamberlain et al, 1970) or 1251-digoxin (Horgan and Riley, 1973), employ adsorption of the free fraction onto dextran-coated (1969) 'H Adsorption of free label onto More than 1 hour 0-2-10-0 dextran-coated charcoal (Herbert, 1965) Chamberlain et al (1970) 'H Dextran-coated charcoal More than 1 hour 0.5-8 0 (ZnSO, + NaOH) Lader et al (1972) 'H Albumin-coated charcoal More than 1 hour 05-40 Barrett and Cohen (1972) 'H…”

Section: Discussionmentioning

confidence: 99%

A rapid, simple assay for digoxin

1974

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SYNOPSISAn extremely rapid radioimmunoassay for digoxin is described which is precise over the range of concentrations required to determine whether, or not, a patient has digoxin toxicity. The assay is based on the use of 125-iodine-labelled digoxin and of a gel equilibration technique for the separation of antibody-bound and free digoxin. The results obtained compare closely with those by a conventional radioimmunoassay and the technique is sufficiently simple to enable its performance by relatively inexperienced laboratory staff.Since Butler and Chen (1967) described a method for producing antibodies to the cardiac glycosides, many radioimmunoassays for digoxin have been developed. It is now becoming apparent that a variety of such assays are needed according to the nature and urgency of a request.1 A rapid but relatively insensitive assay is required for urgent samples from patients with suspected digoxin toxicity. Such requests are relatively infrequent; however, results are required with a minimum of delay and the assay should cover the clinically important range of concentrations (1 to 4 ng/ml). Furthermore, the assay should, ideally, be so simple technically that it can be performed by laboratory personnel who are inexperienced in radioimmunoassay, since it is unlikely that experienced radioimmunoassayists will always be available.2 A routine assay is required suitable for large numbers of samples from patients maintained on digoxin to ensure adequate control of their therapy. In this situation a rapid result is unnecessary but attention should be given to precision and cost.3 A highly sensitive assay is required suitable for research samples and for the very small blood samples obtained by heelprick in neonatal practice.This paper describes an assay developed to fulfil the requirements of the first type. It is based on the use of 1251-digoxin as tracer and of a gel equilibration technique for the separation of antibodybound and free digoxin. Materials and MethodsDixogin standards, ranging from 05 to 8-0 ng/ml, were made up in digoxin-free plasma from stock

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