The Mastitis Pathogens Culture Collection

Dufour, Simon; Labrie, Josée; Jacques, Mario

doi:10.1128/mra.00133-19

Cited by 59 publications

(54 citation statements)

References 2 publications

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“…On the sampled farms, this was practiced on average twice a week. Unhygienic bedding materials and heavily soiled cattle shed are potential risk factors for transmission of causal microorganisms for disease in postpartum cows, of which endometritis is a prevalent fertility disease [30,31]. Furthermore, high mastitis prevalence (76.2%) has been attributed [32] to inadequate biosecurity measures on zero-grazing dairy farms in Rwanda.…”

Section: Discussionmentioning

confidence: 99%

Perception of farmers about endometritis prevention and control measures for zero-grazed dairy cows on smallholder farms in Rwanda

et al. 2020

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Background: Endometritis is a prevalent uterine disease in postpartum cows. The disease reduces fertility performance and milk yield, and subsequently, productivity and profitability of dairy farms. The reduction in performance is associated with considerable economic losses on dairy farms. Smallholder farmers are likely to incur considerable economic losses from the disease where they lack knowledge of effective prevention and control measures for the disease. This study used farmer's perspectives to determine the effectiveness of different management interventions (MIs) for endometritis prevention and control on smallholder farms in Rwanda practicing dairy zero-grazing. The best-worst scaling (BWS) choice method was applied that relied on past 1 year recall data obtained from 154 farmers. These farmers were identified through snowball sampling in a cross-sectional study. Results: Of the 20 MIs evaluated, 12 scored highly for effectiveness. The top four most effective are: avoiding sharing equipment with neighbouring farms (45.5%), consulting animal health service provider about disease treatment (31.8%), keeping cows in a clean and dry shed (26.7%), and selecting sires based on calving ease (26.6%). The MIs considered least effective were: maintaining clean transition cow housing (35.1%), removal of fetal membrane immediately after passing (33.1%), disinfecting the equipment used in calving assistance before and after use (32.5%), and selecting sires with low percent stillbirths (29.2%). Conclusion: This study has demonstrated the application of BWS object case method in understanding the MIs that farmers consider are most effective in the prevention and control of endometritis disease in the dairy herds. The MIs are on-farm biosecurity and hygiene, seeking veterinary services for disease treatment and selecting sires for ease of calving. These MIs should be considered for prioritization in extension services and research to continuously improve and enhance their practical application on smallholder dairy farms.

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Section: Discussionmentioning

confidence: 99%

Perception of farmers about endometritis prevention and control measures for zero-grazed dairy cows on smallholder farms in Rwanda

et al. 2020

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“…The Canadian Bovine Mastitis Research Network maintains a culture collection of mastitis isolates that were collected from 91 dairy farms across Canada over a 2-year period (2007 and 2008) ( 11 ). All strains sequenced in this project were obtained from this collection.…”

Section: Announcementmentioning

confidence: 99%

“…Briefly, the isolates were cultured from raw milk samples plated on biplates containing a 1:1 mixture of Columbia agar with 5% sheep blood and MacConkey agar. Standard biochemical tests were performed to confirm that the isolates were E. coli (lactose and indole positive and oxidase and citrate negative) ( 11 ). To extract DNA for sequencing, E. coli was grown overnight on tryptic soy agar (TSA) and a well-isolated single colony was selected for DNA isolation.…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequences of 113 Escherichia coli Strains Isolated from Intramammary Infections in Dairy Cattle

Jung

Park

Ruffini

et al. 2021

Microbiol Resour Announc

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“…Common bacterial pathogens in cattle include S. aureus (mastitis), and M. haemolytica and P. multocida (BRDC) [38] [39]. To assess the ability of LTC to activate macrophage bactericidal activity against these important cattle bacterial pathogens, MDM were pre-treated with LTC and induction of bactericidal was assessed.…”

Section: Fig 2 Treatment Of Monocyte Derived Macrophages With Ltc Stmentioning

confidence: 99%

Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant

et al. 2020

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Alternatives to antibiotics for prevention of respiratory tract infections in cattle are urgently needed given the increasing public and regulatory pressure to reduce overall antibiotic usage. Activation of local innate immune defenses in the upper respiratory tract is one strategy to induce non-specific protection against infection with the diverse array of viral and bacterial pathogens associated with bovine respiratory disease complex (BRDC), while avoiding the use of antibiotics. Our prior studies in rodent models demonstrated that intranasal administration of liposome-TLR complexes (LTC) as a non-specific immune stimulant generated high levels of protection against lethal bacterial and viral pathogens. Therefore, we conducted studies to assess LTC induction of local immune responses and protective immunity to BRDC in cattle. In vitro, LTC were shown to activate peripheral blood mononuclear cells in cattle, which was associated with secretion of INFγ and IL-6. Macrophage activation with LTC triggered intracellular killing of Mannheimia hemolytica and several other bacterial pathogens. In studies in cattle, intranasal administration of LTC demonstrated dose-dependent activation of local innate immune responses in the nasopharynx, including recruitment of monocytes and prolonged upregulation (at least 2 weeks) of innate immune cytokine gene expression by nasopharyngeal mucosal cells. In a BRDC challenge study, intranasal administration of LTC prior to pathogen exposure resulted in significant reduction in both clinical signs of infection and disease-associated euthanasia rates. These findings indicate that intranasal administration of a non-specific innate immune stimulant can be an effective method of rapidly generating generalized protection from mixed viral and bacterial respiratory tract infections in cattle.

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The Mastitis Pathogens Culture Collection

Cited by 59 publications

References 2 publications

Perception of farmers about endometritis prevention and control measures for zero-grazed dairy cows on smallholder farms in Rwanda

Perception of farmers about endometritis prevention and control measures for zero-grazed dairy cows on smallholder farms in Rwanda

Draft Genome Sequences of 113 Escherichia coli Strains Isolated from Intramammary Infections in Dairy Cattle

Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant

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