Draft Genome Sequences of 113 Escherichia coli Strains Isolated from Intramammary Infections in Dairy Cattle

Jung, Dongyun; Park, So-Youn; Ruffini, Janina; Dufour, Simon; Ronholm, Jennifer

doi:10.1128/mra.01464-20

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“…In this study, we assessed the prevalence of AMR and virulence characteristics of 113 E. coli isolates obtained from MPCC using phenotypic assays. Further, the presence of genes corresponding to the identified AMR and virulence characteristics were verified from the whole genome data reported recently [ 13 , 14 ]. Knowledge about the prevalence of AMR and virulence factors involved in the survival and persistence of E. coli causing IMI is pivotal for clinical management of disease as well as for designing new therapeutic agents.…”

Section: Introductionmentioning

confidence: 54%

“…Extraction and quantification of DNA of each isolate, DNA library preparation, whole-genome sequencing, assembly, and annotation of sequenced reads were conducted as previously described (supplementary table S3 ) [ 13 , 14 ]. Assembly was conducted using ProkaryoteAssembly version 0.1.6 ( https://github.com/bfssi-forest-dussault/ProkaryoteAssembly ) [ 42 – 44 ].…”

Section: Methodsmentioning

confidence: 99%

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Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada

et al. 2021

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Background Bovine mastitis is the most common infectious disease in dairy cattle with major economic implications for the dairy industry worldwide. Continuous monitoring for the emergence of antimicrobial resistance (AMR) among bacterial isolates from dairy farms is vital not only for animal husbandry but also for public health. Methods In this study, the prevalence of AMR in 113 Escherichia coli isolates from cases of bovine clinical mastitis in Canada was investigated. Kirby-Bauer disk diffusion test with 18 antibiotics and microdilution method with 3 heavy metals (copper, zinc, and silver) was performed to determine the antibiotic and heavy-metal susceptibility. Resistant strains were assessed for efflux and ß-lactamase activities besides assessing biofilm formation and hemolysis. Whole-genome sequences for each of the isolates were examined to detect the presence of genes corresponding to the observed AMR and virulence factors. Results Phenotypic analysis revealed that 32 isolates were resistant to one or more antibiotics and 107 showed resistance against at least one heavy metal. Quinolones and silver were the most efficient against the tested isolates. Among the AMR isolates, AcrAB-TolC efflux activity and ß-lactamase enzyme activities were detected in 13 and 14 isolates, respectively. All isolates produced biofilm but with different capacities, and 33 isolates showed α-hemolysin activity. A positive correlation (Pearson r = + 0.89) between efflux pump activity and quantity of biofilm was observed. Genes associated with aggregation, adhesion, cyclic di-GMP, quorum sensing were detected in the AMR isolates corroborating phenotype observations. Conclusions This investigation showed the prevalence of AMR in E. coli isolates from bovine clinical mastitis. The results also suggest the inadequacy of antimicrobials with a single mode of action to curtail AMR bacteria with multiple mechanisms of resistance and virulence factors. Therefore, it calls for combinatorial therapy for the effective management of AMR infections in dairy farms and combats its potential transmission to the food supply chain through the milk and dairy products.

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Section: Introductionmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada

et al. 2021

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Draft Genome Sequences of 113 Escherichia coli Strains Isolated from Intramammary Infections in Dairy Cattle

Abstract: Escherichia coli is one of the most common etiological agents responsible for clinical bovine mastitis. Here, we report the draft genome sequences and annotations of 113 E. coli strains that were isolated from Holstein cows with intramammary infections in Canada.

Cited by 1 publication

References 15 publications

Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada

Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada

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