The Manual Polybrene Test: A Simple and Rapid Procedure for Detection of Red Cell Antibodies

Lalezari, Parviz; Jiang, An Fu

doi:10.1046/j.1537-2995.1980.20280169962.x

Cited by 84 publications

(52 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, minor crossmatching was given up since the introduction of antibody screening for donors. Additives such as bovine albumin, low ionic st rength media, 10 polybrene, 11 and polyethylene-glycol 12 (PEG) and enzyme 13 treated red blood cells were used to enhance agglutination and to further shorten incubation times. In the last few years, pretransfusion testing practices have shifted from tube to CAT.…”

Section: Discussionmentioning

confidence: 99%

Red cell incompatibility due to antibody against ingredient in column matrix: A rare entity

Rajendran¹,

Krishna²,

Suresh³

et al. 2016

J Clin Sci Res

View full text Add to dashboard Cite

An essential goal in transfusion medicine is that transfused blood be compatible with the patient. Several problems arise while performing pre-transfusion compatibility testing. Rarely, in vitro reactions not due to blood group antibodies are sometimes encountered which can pose difficulty in routine immunohematology work up. Though these antibodies are clinically insignificant, proper work-up is indicated, before labelling such antibodies as clinically insignificant. In the report we describe a rare case wherein the patient had an antibody against the ingredients of the matrix of column agglutination. Key words: Red cell antibody, Gel column, Antibody identification

show abstract

Section: Discussionmentioning

confidence: 99%

Red cell incompatibility due to antibody against ingredient in column matrix: A rare entity

Rajendran¹,

Krishna²,

Suresh³

et al. 2016

J Clin Sci Res

View full text Add to dashboard Cite

show abstract

“…Each serum sample was screened by 2-stage papain technique (2-3% red cell suspension of papain-treated cells) at 37°C, manual polybrene technique as described by Lalezari and Jung [2], continued through to the indirect antiglobulin technique (IAT) phase, and by con ventional normal ionic strength [red cells suspended in phosphate-buf fered saline (PBS)-pH 7.0±0.2] indirect antiglobulin technique (NIS-IAT). The IAT was performed using 4 voi of patient's serum and 1 voi of a 5% red cell suspension, followed by 45 min incubation at 37°C, after which the reaction mixtures were read for agglutination prior to shing in order to look for agglutinating IgM antibodies.…”

Section: Serological Testsmentioning

confidence: 99%

A Prospective Study of the Incidence of Red Cell Allo-lmmunisation following Transfusion

Redman¹,

Regan²,

Contreras³

1996

Vox Sanguinis

View full text Add to dashboard Cite

To establish the incidence and timing of red cell allo-immunisation following transfusion, pretransfusion and serial post-transfusion samples were screened for allo-antibodies in a total of 452 patients who had undergone elective surgery. Antibody screening was performed by 2-stage papain, manual polybrene and indirect antiglobulin techniques (1AT). Red cell allo-antibodies were found in 42 patients and 38 of these (8.4% overall) demonstrated antibodies only after transfusion; 76% of them had Rh specificity. This rate of red cell allo-immunisation is higher than what would be expected if transfused patients were tested only once post-transfusion, as has been the case in several previous studies. For the type of patients studied, this finding may not be of clinical relevance at present because most patients undergoing elective surgery do not require further transfusion in their lifetime. However, this is changing with the longer life expectancy of the population and the increased probability of repeat surgery. Twenty-two (58%) of the antibodies were initially detected by the 2-stage papain and/or polybrene techniques, when the IAT was negative, although later 19 became positive by I AT; this added sensitivity of techniques other than the IAT, to detect early alloimmunisation may be relevant in pretransfusion testing to prevent haemolytic transfusion reactions in patients requiring repeated transfusions.

show abstract

“…Rh-negative women in whom an Rh antibody was detected only by an AutoAnalyzer method [10,11], but not by P2 or IDAT, were not eligible for the trial. Because 86% of 236 such women not given RhIG dur ing pregnancy and delivering Rh-positive babies had no increase in the strength of their Rh antibodies, and since similar antibodies have been found in non-transfused men [12], we believe that most women with Rh anti bodies demonstrable by AutoAnalyzer only are not truly Rh immunized.…”

Section: Clinical Trialmentioning

confidence: 99%

Reversal of Rh Alloimmunization

Bowman¹,

Pollock²

1984

Vox Sanguinis

View full text Add to dashboard Cite

The Rh Laboratory could find no evidence that Rh immune globulin (RhIG) reduced the incidence of progression of Rh immunization in a clinical study of 36 weakly Rh-immunized women given 300 µg of RhIG at 6-week intervals during pregnancy and after delivery, and in a retrospective study of the natural course of weak Rh immunization in pregnant Rh-negative women not given RhIG and others given RhIG at 28 weeks` gestation and/or after delivery of an Rh-positive baby. Although the failure of about one-third of weakly immunized Rh pregnant women to show any progression of their Rh immunization may be due to relative immune unresponsiveness in some, we believe that most do not have any increase in their Rh immunization because they have no further exposure to Rh-positive fetal red cells. Serial Kleihauer testing of pregnant women at 2-week intervals is being carried out to test this hypothesis.

show abstract

The Manual Polybrene Test: A Simple and Rapid Procedure for Detection of Red Cell Antibodies

Cited by 84 publications

References 6 publications

Red cell incompatibility due to antibody against ingredient in column matrix: A rare entity

Red cell incompatibility due to antibody against ingredient in column matrix: A rare entity

A Prospective Study of the Incidence of Red Cell Allo-lmmunisation following Transfusion

Reversal of Rh Alloimmunization

Contact Info

Product

Resources

About