The Male Stem Cell Niche: Insights from Drosophila and Mammalian Model Systems

Papagiannouli, Fani; Lohmann, Ingrid

doi:10.1007/978-3-319-21705-5_12

Cited by 2 publications

(3 citation statements)

References 174 publications

(280 reference statements)

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“…Based on the signals they receive, cells adapt their intrinsic features and signalling machinery to achieve a coordinated output. In the Drosophila testis, cell communication via close-range signalling exchange is established between the germline and the somatic lineage that is crucial to build a functional testis and produce fertile sperm (Papagiannouli and Lohmann, 2012; Papagiannouli and Lohmann, 2015). At the anterior-most part of the testis is the male stem cell niche, a cluster of non-dividing tightly packed somatic cells that build the so-called hub.…”

Section: Introductionmentioning

confidence: 99%

Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

Alvarez,

Papagiannouli

2024

Preprint

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Cell communication via signalling exchange plays a pivotal role in multicellular development for building functional tissues and organs. In theDrosophilatestis, a pair of somatic cyst cells (CCs) encapsulate the germline that differentiates through close-range EGFR signalling activation. The conserved Dlg/Scrib/Lgl cortical polarity complex and clathrin-mediated endocytosis attenuate EGFR signalling in CCs and loss of their function leads to EGFR overactivation and non-autonomous death of the neighbouring germ cells. Here we show that EGFR overactivation results in upregulation of JNK and p38 signalling in CCs and ROS levels in the germ cells that are destined to die. Our data uncover a bidirectional feedback between JNK signalling and ROS who regulate each other within the CC-germline microenvironment, while reducing the levels of either JNK or ROS restores germ cell survival. This study provides a framework of how polarity and cellular trafficking regulate the output of multiple signalling responses cell-intrinsically and in adjacent cells, to coordinate tissue-specific responses and maintain homeostasis.

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Section: Introductionmentioning

confidence: 99%

Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

Alvarez,

Papagiannouli

2024

Preprint

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“…Tissue-specific stem cells are regulated through their own intrinsic program as well as by signals emanating from their microenvironment. Tissues rely on stem cells to constantly replenish their cellular populations under normal conditions but also activate proliferation of resident stem cell upon damage, injury or aging to facilitate tissue regeneration (Fuller and Spradling, 2007;Matunis et al, 2012;Papagiannouli and Lohmann, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…After completion of TA divisions, spermatogonia become spermatocytes, undergo pre-meiotic DNA replication that turns on the transcriptional program for terminal differentiation, and undergo meiosis. Cyst cells co-differentiate with the germ cells they enclose, grow enormously in size, elongate and encapsulate them throughout their differentiation steps up to individualization and sperm production in the adult testis (Fuller, 1993;Papagiannouli and Lohmann, 2015).…”

Section: Introductionmentioning

confidence: 99%

Nuclear export in somatic cyst cells controls cyst cell-germline coordination and germline differentiation in theDrosophilatestis

Papagiannouli

Fuller

Lohmann

2018

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Nucleocytoplasmic communication is crucial for proper cell function and coordination of intrinsic cues with signaling responses emanating from the neighboring cells and the local tissue microenvironment. In the Drosophila male germline system, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small cyst, the functional unit of differentiation. Here we show that the peripheral nucleoporins Nup62, Nup214 and Nup88, and the exportin Emb are critically required in cyst cells to maintain cyst cell survival and germline encapsulation in order to protect cyst cell-germline communication and promote germ cell differentiation. Knockdown of nup62, emb, nup214 or nup88 in cyst cells leads to cell-autonomous defects in mRNA export, and cell non-autonomous overproliferation of early germ cells in the absence of cyst cell-derived differentiation signals. Suppression of apoptosis can reverse cyst cell elimination and partially restored those defects. Interestingly, overexpression of the Drosophila Profilin gene chickadee can rescue cyst cell survival and restore germline encapsulation and differentiation, by counteracting Ntf-2 mediated export, suggesting that the function of Profilin in cyst cells is linked to nuclear export.

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The Male Stem Cell Niche: Insights from Drosophila and Mammalian Model Systems

Cited by 2 publications

References 174 publications

Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

Nuclear export in somatic cyst cells controls cyst cell-germline coordination and germline differentiation in theDrosophilatestis

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