The male reproductive system and the effect of an extract of a medicinal plant (Hypericum perforatum) on the labeling process of blood constituents with technetium-99m

Abstract

Hypericum perforatum (hiperico) is a plant that has been used to treat diseases and also inhibits rat and human vas deferens contractility. In nuclear medicine, stannous chloride (SnCl2) is used as a reducing agent to obtain radiopharmaceuticals labeling with technetium-99m. As the SnCl2 seems to have adverse effects related with the reproductive performance of male rabbits as well as the human consumption of hiperico might affect sexual function. In the present work, consistent results show significant changes on the blood constituents labeled by technetium-99m obtained from young rats under the effect of an hiperico extract as opposed to blood samples equally treated taken from elderly rat.. Supposedly, this extract could protect the male reproductive system against action of SnCl2 at least in young rats. The findings described in this work allow introducing a simple assay to evaluate the action of products that could interfere with the male reproductive system.<br>Hypericum perforatum (hiperico) tem sido utilizado para tratar diferentes distúrbios e também inibir a contractilidade do ducto deferente em ratos e em humanos. Na medicina nuclear, o cloreto estanoso (SnCl2) é usado como um agente redutor para obter radiofármacos marcados com tecnécio-99m. Como o SnCl2 parece acarretar efeitos indesejáveis relacionados com o desempenho reprodutivo de coelhos machos e o hiperico pode afetar a função sexual em humanos, o objetivo desse trabalho é apresentar resultados sobre o efeito de um extrato de hiperico na marcação de constituintes sangüíneos com o tecnécio-99m retirados de ratos jovens e idosos. O hiperico parece alterar a marcação de constituintes sangüíneos com tecnécio-99m isolados de sangue de animais jovens. Embora, esse resultado não seja observado em ratos idosos. Provavelmente, o extrato poderia apresentar uma ação protetora para o sistema reprodutivo contra a ação do SnCl2, pelo menos em ratos jovens. Os resultados descritos nesse trabalho permitem introduzir um ensaio simples para avaliar a ação de produtos que poderiam interferir com o sistema reprodutor masculino

Effect of Karas (Aquilaria malaccensis) on Male Reproductive Organs and Sperm Quality in Adult Sprague Dawley Rats

“…For example, excess of alcohol consumption may result in uncontrolled production of reactive oxygen species (ROS) and a rapid loss in antioxidant capacity ( Agarwal et al 2020 ). In addition, disturbance in the intricate balance between the production and scavenging of ROS is a common underlying mechanism associated with male infertility ( Agarwal et al 2006 ; Santos-Filho et al 2007 ). This improper balance between ROS generation and scavenging activity leads to oxidative stress and damaging the sperm cells ( Jimoh et al 2021 ).…”

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

“…Thus, we decided to use this procedure, in order to investigate genotoxicity and repair activity, using SnCl 2 , as positive control, and E. coli base excision repair. Stannous chloride (SnCl 2 ) is used in different areas of human daily life, for example in nuclear medicine as a reducing agent of technetium-99m, with the aim to radiolabel pharmaceutical products (Saha, 1998;Santos-Filho et al, 2007). The SnCl 2 biological effects have been studied by many authors in the last decade Cabral et al, 1998;Dantas et al, 1999;De Mattos et al, 2000;De Mattos et al, 2005).…”