The major histocompatibility complex of the chicken

Pazderka, F.; Longenecker, B. M.; Law, G. R. J.; Ruth, R. F.

doi:10.1007/bf01572280

Cited by 74 publications

(30 citation statements)

References 82 publications

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“…We have produced a pane/ of alloantisera which react specifically to B~, B2, B~3, B~4, B~5, BI9, or B?I alloantigens. These are original Hy line alleles and their serology as well as associated physiological traits have been extensively discussed elsewhere (Longenecker et aL 1970, 1972, 1975, Pazderka et al 1975a. White blood cells (WBC) were prepared as described previously (Wong et al 1972) and suspended in PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Other problems with the agglutination test are the dependence of the agglutination reaction upon IgM antibodies and the relative insensitivity of the technique. Our approach to MHC typing in the chicken involves the use of alloantisera which are exhaustively absorbed so that they are operationally monospecific (Pazderka et al 1975a). This approach allows us to detect identical MHC alleles in several unrelated populations of chickens from all over the world (Pazderka et aL 1975b, Longenecker et al 1977.…”

Section: Introductionmentioning

confidence: 99%

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Histocompatibility typing by cellular radioimmunoassay

et al. 1978

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A quantitative cellular radioimmunoassay (CRIA) for histocompatibility typing is described. Chicken red blood cells (RBC) were incubated in microtiter plates with specific anti-MHC (B) alloantisera and the alloantibody bound measured indirectly by a second binding step with(125)I-labeled rabbit anti-chicken IgG. The assay is objective, highly consistent, and three to four orders of magnitude more sensitive than conventional hemagglutination assays. The new CRIA was used to detect minor subpopulations of cells in artificial cell mixtures; as few as 1% of relevant cells were easily detected. Erythrocyte chimerism was induced following the injection of B(2)/B(2) chicken embryos with B(15)/B(21) embryonic stem cells. Five weeks after hatching, erythrocyte chimerism was precisely quantitated by comparing the reaction of RBC from the putative chimeras with artificial cell mixtures using specific anti-B(15)/B(21) alloantisera. The percent varied from 13-40% in 13 chimeric animals. The new CRIA was also used for the sensitive detection of tumor-specific antigens on a T-cell lymphoma. An unexpected finding was that anti-B(15) alloantibody bound almost as well to B(15)/B(21) heterozygous RBC as to B(15)/B(15) homozygous cells, suggesting that either the concentration or the steric arrangement of B(15) alloantigen at the erythrocyte surface may not conform to conventional expectations.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histocompatibility typing by cellular radioimmunoassay

et al. 1978

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“…Differences in susceptibility to MDV-induced tumorigenesis have also been described for chickens of the regional Poultry Research Laboratory (RPRL) sublines 6 and 7 (Crittenden et al 1972), which are serologically identical with respect to the B locus (genotype B2/B2; Pazderka et al 1975). Differences in immune response to protein and polypeptide antigens, as well as to dinitrophenol (DNP) have been described for the RPRL6 and RPRL7 lines (Palladino and Gilmour, unpublished, cited in Gilmour et al 1976).…”

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confidence: 90%

“…The major histocompatibility complex of the chicken (Pazderka et al 1975) resembles the mouse H-2 region in controlling many phenomena related to adaptive immune responsiveness. Immune response (Ir) loci within the B complex control cell-mediated and humoral immune responses to defined antigens (G/inther et al 1974(G/inther et al , Benedict et al 1975(G/inther et al , 1977.…”

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confidence: 99%

Differential mortality and lesion responses to reticuloendotheliosis virus infection in Marek's disease-resistant and susceptible chicken lines

et al. 1978

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“…The B-G region is at least as polymorphic as the other regions, but its function remains unknown [12]. The B-L/ B-F regions have all the attributes of the MHC, which are comparable to those of mammals and determines rapid allograft rejection, strong mixed lymphocyte reaction (MLR) and cellular cooperation in the immune response [26,38,39].…”

Section: Introductionmentioning

confidence: 99%