The major basement membrane components localize to the chondrocyte pericellular matrix — A cartilage basement membrane equivalent?

Kvist, Alexander J.; Nyström, Alexander; Hultenby, Kjell; Sasaki, Takako; Talts, Jan F.; Aspberg, Anders

doi:10.1016/j.matbio.2007.07.007

Cited by 90 publications

(103 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In adult tissues, WARP is highly restricted to the chondrocyte pericellular matrix in articular cartilage and fibrocartilages, where it colocalizes with perlecan and collagen VI (3). Several of the major basement membrane components have been found in the chondrocyte pericellular matrix, suggesting that this structure may be the functional equivalent of a basement membrane in cartilage tissues (4). Consistent with this hypothesis, recent data from our laboratory have demonstrated that WARP is a component of the basement membrane in a limited subset of tissues including the apical ectodermal ridge, the endomysium surrounding muscle fibers, the vasculature of the central nervous system, and the endoneurium of peripheral nerves (5).…”

supporting

confidence: 52%

Mice Lacking the Extracellular Matrix Protein WARP Develop Normally but Have Compromised Peripheral Nerve Structure and Function

Allen

Zamurs

Brachvogel

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

WARP is a recently identified extracellular matrix molecule with restricted expression in permanent cartilages and a distinct subset of basement membranes in peripheral nerves, muscle, and the central nervous system vasculature. WARP interacts with perlecan, and we also demonstrate here that WARP binds type VI collagen, suggesting a function in bridging connective tissue structures. To understand the in vivo function of WARP, we generated a WARPdeficient mouse strain. WARP-null mice were healthy, viable, and fertile with no overt abnormalities. Motor function and behavioral testing demonstrated that WARP-null mice exhibited a significantly delayed response to acute painful stimulus and impaired fine motor coordination, although general motor function was not affected, suggesting compromised peripheral nerve function. Immunostaining of WARP-interacting ligands demonstrated that the collagen VI microfibrillar matrix was severely reduced and mislocalized in peripheral nerves of WARP-null mice. Further ultrastructural analysis revealed reduced fibrillar collagen deposition within the peripheral nerve extracellular matrix and abnormal partial fusing of adjacent Schwann cell basement membranes, suggesting an important function for WARP in stabilizing the association of the collagenous interstitial matrix with the Schwann cell basement membrane. In contrast, other WARP-deficient tissues such as articular cartilage, intervertebral discs, and skeletal muscle showed no detectable abnormalities, and basement membranes formed normally. Our data demonstrate that although WARP is not essential for basement membrane formation or musculoskeletal development, it has critical roles in the structure and function of peripheral nerves. (3), and in the present study we identify type VI collagen as a ligand for WARP. WARP has a restricted distribution in developing cartilage tissues, where it is expressed at sites of joint cavitation and articular cartilage formation rather than cartilage structures that will undergo endochondral ossification (3). In adult tissues, WARP is highly restricted to the chondrocyte pericellular matrix in articular cartilage and fibrocartilages, where it colocalizes with perlecan and collagen VI (3). Several of the major basement membrane components have been found in the chondrocyte pericellular matrix, suggesting that this structure may be the functional equivalent of a basement membrane in cartilage tissues (4). Consistent with this hypothesis, recent data from our laboratory have demonstrated that WARP is a component of the basement membrane in a limited subset of tissues including the apical ectodermal ridge, the endomysium surrounding muscle fibers, the vasculature of the central nervous system, and the endoneurium of peripheral nerves (5). The principal components of basement membranes are type IV collagen, laminins, nidogens, and proteoglycans including perlecan; however, the composition, structure, and biological properties of basement membranes can differ considerably between different tissues (6,...

show abstract

supporting

confidence: 52%

Mice Lacking the Extracellular Matrix Protein WARP Develop Normally but Have Compromised Peripheral Nerve Structure and Function

Allen

Zamurs

Brachvogel

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Increasing evidence suggests that PCM contains laminin (LM), collagen type IV, nidogen and perlecan, which form the functional equivalent of a basement membrane (Kvist et al, 2008). Compared to the kidney, an organ highly enriched in glomerular basement membranes, articular chondrocytes exhibited significantly higher expression of LM α4, LM β1 and nidogen-2, despite comparable levels of LM α1, LM α2 and LM α5 (Kvist et al, 2008). The distribution and abundance of basement membrane components in cartilage are age-dependent.…”

Section: Introductionmentioning

confidence: 99%

“…The distribution and abundance of basement membrane components in cartilage are age-dependent. A gradual shift is distinct, from a diffuse expression in the territorial and interterritorial www.ecmjournal.org matrices of newborn mice toward a pericellular localization in mature cartilage, which then becomes less distinct once reaching old age (Kvist et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In articular cartilage, the staining of basement membrane proteins in the PCM was most prominent around cells of the cartilage superficial layer (Kvist et al, 2008), which is known as a niche for chondroprogenitors (Candela et al, 2014). Furthermore, a recent study found enhanced LM α1, LM α5 and nidogen-2 in the PCM of osteoarthritic chondrocytes, suggesting that laminin promotes restoration of chondrocyte phenotypes (Schminke et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The role of laminins in cartilaginous tissues: from development to regeneration

Sun¹,

Wang²,

Toh³

et al. 2017

eCM

View full text Add to dashboard Cite

As a key molecule of extracellular matrix, laminin provides a delicate microenvironment for cell functions. Recent findings suggest that laminins expressed by cartilage-forming cells (chondrocytes, progenitor cells and stem cells) could promote chondrogenesis. However, few papers outline the effect of laminins on providing a favorable matrix microenvironment for cartilage regeneration. In this review, we delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. We also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration and survival. Furthermore, we scrutinized the potential influence of various laminin isoforms on cartilage-forming cells' proliferation and chondrogenic differentiation. With this information, we hope to facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration.

show abstract

“…55 Furthermore, the AC pericellular matrix protein Laminin-111 may bind to and activate synovial fibroblasts in the presence of TGF-b1, which was found to result in IL-6 and IL-8 secretion, a mechanism by which synovial fibroblasts may contribute to disease pathology outside periods of acute inflammation. 56,57 Synovial fibroblasts also upregulate expression of vascular cell adhesion molecule-1 (VCAM-1) in response to chemokine (C-C motif ) ligand 2 (CCL2) and CCN family member 4, both of which are detected in synovial fluid of patients with OA. This process may facilitate the adhesion of mononuclear cells to the site of inflammation.…”

Section: Synovial Fibroblastsmentioning

confidence: 99%

Immune Modulation to Improve Tissue Engineering Outcomes for Cartilage Repair in the Osteoarthritic Joint

Fahy

Farrell

Ritter

et al. 2015

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

The major basement membrane components localize to the chondrocyte pericellular matrix — A cartilage basement membrane equivalent?

Cited by 90 publications

References 62 publications

Mice Lacking the Extracellular Matrix Protein WARP Develop Normally but Have Compromised Peripheral Nerve Structure and Function

Mice Lacking the Extracellular Matrix Protein WARP Develop Normally but Have Compromised Peripheral Nerve Structure and Function

The role of laminins in cartilaginous tissues: from development to regeneration

Immune Modulation to Improve Tissue Engineering Outcomes for Cartilage Repair in the Osteoarthritic Joint

Contact Info

Product

Resources

About