The role of laminins in cartilaginous tissues: from development to regeneration

Sun, Yu; Wang, T L; Toh, Wei Seong; Pei, Ming

doi:10.22203/ecm.v034a03

Cited by 40 publications

(28 citation statements)

References 106 publications

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“…Nevertheless, laminins mainly dictate the structure and assembly of MB [77]. Moreover, these glycoproteins play a crucial role in cell migration, adhesion, differentiation, and wound healing [78][79][80][81]. They also participate in early embryonic development and organogenesis.…”

Section: Lamininsmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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Section: Lamininsmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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“…Laminins are a family of heterotrimeric ECM proteins 33 . Previous studies have shown the expressions of specific laminins were differentially regulated during development, adhesion, migration and regeneration [34][35][36] . In our study, LAMA2 was observed to be up-regulated in KBD group in comparison to OA.…”

Section: Discussionmentioning

confidence: 98%

Proteomic analysis of knee cartilage reveals potential signaling pathways in pathological mechanism of Kashin-Beck disease compared with osteoarthritis

Lei

Amhare

Wang

et al. 2020

Sci Rep

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The pathological mechanism of Kashin-Beck disease (KBD), an endemic osteoarthritic disease, remains to be poorly understood. This study was designed to identify signaling pathways and crucial proteins involved in the pathological mechanism of KBD compared with osteoarthritis (OA). The knee cartilage samples were collected from gender-and age-matched KBD (n = 9) and OA (n = 9) patients. After preprocessing, samples were labeled with Tamdem Mass Tags 6plex multiplex kit, and analyzed by liquid chromatography-tandem mass spectrometry. Proteomic results were analyzed with gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interactions (PPI). The differential abundance proteins from KBD and OA were validated using western blot analysis. As a result, A total number of 375 proteins were identified to have differential abundance between KBD and OA, of which 121 and 254 proteins were observed to be up-regulated or down-regulated in KBD group. GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. In conclusion, our results demonstrated that compared with OA, the differential abundance proteins and signaling pathways may contribute to the occurrence and development of joint damage in KBD. Further investigation of their regulative roles and interaction may provide new insights into the pathological mechanisms and therapeutic targets for KBD.Osteoarthritis (OA) is a progressive, degenerative joint disease. It is the major cause of knee pain and locomotor disability worldwide 1,2 . The WHO Scientific Groupon Rheumatic Diseases estimates that 10% of the world's population who are 60 years or older have significant clinical problems attributed to OA 3 . Unlike OA, Kashin-Beck disease (KBD) is a chronic and serious endemic osteoarticular disease, which has been in high prevalence and morbidity in Eastern Siberia of Russia, Northeast China to Sichuan-Tibet Plateau, and North Korea 4,5 . According to the statistics, there were 1.04 billion people in risk of KBD and 0.57 million patients with KBD in China 6 . However, the etiology and pathological mechanisms of KBD are still waiting to be uncovered 7 , which lessen the chances of further effective prevention and treatment measures for the current KBD patients.

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“…In contrast, NID2 and COL4A5 were significantly more highly expressed in ScASCs compared to donor-matched IPFSCs. Given the function of HSPG2 encoding perlecan as the "factotum" in cartilage (Melrose et al, 2008) and the role of laminins in cartilage regeneration (Sun et al, 2017), NID2 and COL4A5 expression might be associated with adipogenic differentiation, which remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Site-Dependent Lineage Preference of Adipose Stem Cells

Wang

Hill

Dzieciątkowska

et al. 2020

Front. Cell Dev. Biol.

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Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depotdependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells.

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The role of laminins in cartilaginous tissues: from development to regeneration

Cited by 40 publications

References 106 publications

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Proteomic analysis of knee cartilage reveals potential signaling pathways in pathological mechanism of Kashin-Beck disease compared with osteoarthritis

Site-Dependent Lineage Preference of Adipose Stem Cells

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