The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens

Optimisation of an extraction technique of fish allergens suitable for detection and diagnosis

“…In a similar observation, Gajewski et al (2009) reported multiple PV bands representing different PV isotypes as different fish species are known to express from two to five PV isotypes (Van Do et al 2003). However, the tests of all three human sera showed here a lack of reactivity to PV (absence of bands around 12 kDa) by immunoblotting.…”

New insights into seafood allergy

“…In addition to parvalbumin, other allergens have been characterized in fish such as collagen [38], from the skin and muscle tissue, as well as the hormone, vitellogenin [39][40][41][42], found particularly in fish roe (caviar) ( Table 4 [ [43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58]). It is of note that crustacean and mollusk allergens do not cross-react with fish allergens.…”

Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin

“…4,5 The first identified fish allergen is parvalbumin of cod Gadus callarias , a calcium-binding sarcoplasmic protein with a molecular mass of approximately 12 kDa. 6,7 Subsequent immunoblotting studies 2,8-10 and molecular studies [11][12][13][14][15][16][17][18] have established that parvalbumin represents the major and cross-reactive allergen in common with various species of fish, although minor allergens, such as collagen [19][20][21] and aldehyde phosphate dehydrogenase, 22 have been detected simultaneously.…”