The maize auxotrophic mutant orange pericarp is defective in duplicate genes for tryptophan synthase beta.

Wright, Allen D.; Moehlenkamp, Cynthia; Perrot, Gary H.; Neuffer, M. G.; Cone, Karen C.

doi:10.1105/tpc.4.6.711

Cited by 59 publications

(26 citation statements)

References 20 publications

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“…multiple duplicated, colinear chromosomal regions (Gaut and Doebley 1997). Previous studies found functional redundancy for maize genes that map to homeologous chromosomal positions (Rhoades 1951;Coe et al 1981;Wright et al 1992;Mena et al 1996;Scanlon et al 1996), indicating that the duplicated genes have identical or partially overlapping functions. For the later cases, these are likely caused by subfunctionalization, resulting in diverging tissue-specific expression patterns (Lynch and Force 2000;Langham et al 2004).…”

Section: Resultsmentioning

confidence: 99%

ThePsychedelicGenes of Maize Redundantly Promote Carbohydrate Export From Leaves

Slewinski

Braun

2010

Genetics

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Whole-plant carbohydrate partitioning involves the assimilation of carbon in leaves and its translocation to nonphotosynthetic tissues. This process is fundamental to plant growth and development, but its regulation is poorly understood. To identify genes controlling carbohydrate partitioning, we isolated mutants that are defective in exporting fixed carbon from leaves. Here we describe psychedelic (psc), a new mutant of maize (Zea mays) that is perturbed in carbohydrate partitioning. psc mutants exhibit stable, discrete chlorotic and green regions within their leaves. psc chlorotic tissues hyperaccumulate starch and soluble sugars, while psc green tissues appear comparable to wild-type leaves. The psc chlorotic and green tissue boundaries are usually delineated by larger veins, suggesting that translocation of a mobile compound through the veins may influence the tissue phenotype. psc mutants display altered biomass partitioning, which is consistent with reduced carbohydrate export from leaves to developing tissues. We determined that the psc mutation is unlinked to previously characterized maize leaf carbohydrate hyperaccumulation mutants. Additionally, we found that the psc mutant phenotype is inherited as a recessive, duplicate-factor trait in some inbred lines. Genetic analyses with other maize mutants with variegated leaves and impaired carbohydrate partitioning suggest that Psc defines an independent pathway. Therefore, investigations into the psc mutation have uncovered two previously unknown genes that redundantly function to regulate carbohydrate partitioning in maize.

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Section: Resultsmentioning

confidence: 99%

ThePsychedelicGenes of Maize Redundantly Promote Carbohydrate Export From Leaves

Slewinski

Braun

2010

Genetics

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“…The initial mass spectrometric evidence to support Trp-independent IAA biosynthesis came from labelling studies of the aquatic plant Lemna gibba, which grows floating on water and readily takes up e 5 N]Trp from the medium. The orange pericarp (orp) double mutant lacks both of the TS enzymes and is therefore a Trp auxotroph (Wright et at., 1992). (1991) showed that when the Trp pool was labelled to 98% with e 5 N]Trp very little 15N was incorporated into IAA.…”

Section: Tryptophan-independent Laa Biosynthesismentioning

confidence: 99%

Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana

Ljung

Hull

Kowalczyk

et al. 2002

Auxin Molecular Biology

145

209

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“…A set of primers specific for the orange pericarp-1 (orp-1) gene, which encodes the p subunit of tryptophan synthase (Wright et al, 1992), was used to further standardize the concentration of the dif? ferent samples.…”

Section: Reverse Transcriptase-polymerase Chain Reactionmentioning

confidence: 99%

Light-Dependent Spatial and Temporal Expression of Pigment Regulatory Genes in Developing Maize Seeds

Procissi¹,

Dolfini²,

Ronchi³

et al. 1997

The Plant Cell

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Both light and developmental stimuli are directly involved in the regulation of plant gene expression. In maize, activation of the anthocyanin pathway represents an excellent model system for studying the interactions between an external factor, such as light, and internal factors that regulate plant and seed development. By analyzing in detail the aleurone and pericarp seed layers, different developmental windows for light have been found in the two tissues[mdash]the former in the advanced stages of development and the latter in the early stages of seed development. Transcriptional control of the structural genes involved in anthocyanin deposition within the pericarp is known to be exerted by the Sn and pl genes, whereas the aleurone is controlled by the R and C1 regulatory genes. By using in situ hybridization analysis, we detected tissue-specific expression of Sn and R in the seed layers, revealing a correlation between structural gene activation and anthocyanin accumulation. In addition, RNA gel blot analysis revealed that Sn expression is enhanced by light, whereas the R gene expression is not. However, the light-induced expression of the myb-type genes C1 and pl, detected by reverse transcriptase-polymerase chain reaction, was found to be the limiting factor for conferring the developmental competence of the pericarp and the aleurone layers to light responsiveness.

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The maize auxotrophic mutant orange pericarp is defective in duplicate genes for tryptophan synthase beta.

Cited by 59 publications

References 20 publications

ThePsychedelicGenes of Maize Redundantly Promote Carbohydrate Export From Leaves

ThePsychedelicGenes of Maize Redundantly Promote Carbohydrate Export From Leaves

Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana

Light-Dependent Spatial and Temporal Expression of Pigment Regulatory Genes in Developing Maize Seeds

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