Light-Dependent Spatial and Temporal Expression of Pigment Regulatory Genes in Developing Maize Seeds

Procissi, Antonia; Dolfini, Silvana; Ronchi, Angela; Tonelli, Chiara

doi:10.2307/3870442

Cited by 41 publications

(29 citation statements)

References 27 publications

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“…There are many reports describing the effects of light on anthocyanin gene expression (Fuglevand et al, 1996;Kawabata et al, 1999;Koes et al, 1989;Procissi et al, 1997;Takos et al, 2006;Taylor and Briggs, 1990;Toguri et al, 1993). All these studies demonstrated that anthocyanin gene expression was light inducible.…”

Section: Daylengthmentioning

confidence: 99%

Epistatic Interactions Influencing Anthocyanin Gene Expression in Capsicum annuum

Lightbourn¹,

Stommel²,

Griesbach³

2007

J. Amer. Soc. Hort. Sci.

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Anthocyanin pigmentation in leaves, flowers, and fruit imparts violet to black color and enhances both ornamental and culinary appeal. Shades of violet to black pigmentation in Capsicum annuum L. are attributed to anthocyanin accumulation. Anthocyanin production is markedly influenced by numerous environmental factors, including temperature and light stress. The objective of this study was to determine the genetic basis for differences in C. annuum anthocyanin content in response to varying environments. Growth experiments conducted under controlled environment conditions demonstrated that anthocyanin concentration was significantly higher in mature leaves in comparison with immature leaves under high light (435 μmol·s−1·m−2) conditions. High (30 °C day/25 °C night) versus low (20 °C day/15 °C night) temperature had no significant effect on anthocyanin concentration regardless of leaf maturity stage. Foliar anthocyanin concentration in plants grown under short days (10 h) with low light intensity (215 μmol·s−1·m−2) was significantly less than under long days (16 h) with low light. Under high light intensity, daylength had no effect on anthocyanin content. Three structural genes [chalcone synthase (Chs), dihydroflavonol reductase (Dfr), anthocyanin synthase (Ans)] and three regulatory genes (Myc, MybA , Wd40) were selected for comparison under inductive and noninductive environmental conditions for anthocyanin accumulation. Expression of Chs, Dfr, and Ans was significantly higher in mature leaves in comparison with younger leaves. Consistent with anthocyanin concentration, temperature had no effect on structural gene expression, whereas light positively influenced expression. Under low light conditions, temperature had no effect on Myc, MybA , and Wd40 expression; whereas under high light conditions, temperature only had an effect on MybA expression. The study of anthocyanin leaf pigmentation in C. annuum under inductive and noninductive environments provides a new approach for elucidating the molecular genetic basis of epistatic gene interactions and the resulting phenotypic plasticity.

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Section: Daylengthmentioning

confidence: 99%

Epistatic Interactions Influencing Anthocyanin Gene Expression in Capsicum annuum

Lightbourn¹,

Stommel²,

Griesbach³

2007

J. Amer. Soc. Hort. Sci.

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“…These regulatory proteins belong to the MYB, bHLH and bZIP families, and the genes encoding these transcription factors may themselves be subjected to spatial, temporal and environmental regulation ( Dröge‐Laser et al . 1997 ; Procissi et al . 1997 ).…”

Section: Introductionmentioning

confidence: 99%

The BANYULS gene encodes a DFR‐like protein and is a marker of early seed coat development

Devic¹,

Guilleminot²,

Debeaujon³

et al. 1999

The Plant Journal

221

213

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Summary Mutations in the BANYULS (BAN) gene lead to precocious accumulation of anthocyanins in immature seed coat in Arabidopsis. The ban–1 allele has been isolated from a collection of T‐DNA transformants and found to be tagged by the integrative molecule. The sequencing of wild‐type and two independent mutant alleles confirmed the identity of the gene. Analysis of the full‐length cDNA sequence revealed an open reading frame encoding a 342 amino acid protein which shared strong similarities with DFR and other enzymes of the phenylpropanoid biosynthesis pathway. BAN expression was restricted to the endothelium of immature seeds at the pre‐globular to early globular stages of development as predicted from the maternal inheritance of the phenotype, and therefore represents a marker for early differentiation and development of the seed coat. BAN is probably involved in a metabolic channelling between the production of anthocyanins and pro‐anthocyanidins in the seed coat.

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“…, Vitis vinifera L., V. vinifera x V. labrusca L. (Boss et al, 1996;Gollop et al, 2001;Kobayashi et al, 2001Kobayashi et al, , 2002Sparvoli et al, 1994), and Prunus persica (L.) Batch (Tsuda et al, 2004). Studies led to the conclusion that the pigment is synthesized when at least two kinds of genes are expressed: structural genes, encoding the enzymes catalyzing the reactions of the metabolic pathway, and regulatory genes, encoding the transcription factors controlling their expression (Dooner et al, 1991;Goodrich et al, 1992;Procissi et al, 1997;Quattrocchio et al, 1993). Therefore, any signifi cant genetic occurrence, in even one of the structural or regulatory genes, may result in the loss of production of the pig-ment.…”

mentioning

confidence: 99%

Quantitative Real-time Reverse Transcriptase-PCR Profiling of Anthocyanin Biosynthetic Genes during Orange Fruit Ripening

Cotroneo¹,

Russo²,

Ciuni³

et al. 2006

jashs

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Genes encoding chalcone synthase (CHS), anthocyanidin synthase (ANS), and UDP-glucose-flavonoid 3-O-glucosyltransferase (UFGT), some of the enzymes of anthocyanin biosynthetic pathway, were assayed in two different experiments using quantitative real-time reverse transcriptase (RT)-PCR, in order to test their transcription levels in the flesh of blood and common orange [Citrus sinensis (L.) Osbeck] fruit, and to investigate their role in anthocyanin accumulation in the same tissue. The first experiment compared a blood orange and a common orange cultivar during seven different fruit maturation stages. This was followed by the test of 11 different genotypes at the end of the winter season. Data collected from the first experiment, over the blood orange cultivar, were statistically analyzed using the Pearson correlation coefficient. Results show that CHS, ANS, and UFGT mRNA transcripts are up- and co-regulated in the blood orange cultivar, whereas they are down-regulated in the common orange cultivar. There is evidence of correspondence between the target genes expression level and the content of the pigment assessed. The second test confirms this correlation and shows that enzyme synthesis levels and pigment accumulation, in plants grown under the same environmental conditions, are dependent on the differences occurring among the genotypes tested. These results suggest that the absence of pigment in the common orange cultivars may be caused by the lack of induction on the structural genes expression. This is the first report on the characterization of the relationships between biosynthetic genes expression and fruit flesh anthocyanin content in blood oranges.

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Light-Dependent Spatial and Temporal Expression of Pigment Regulatory Genes in Developing Maize Seeds

Cited by 41 publications

References 27 publications

Epistatic Interactions Influencing Anthocyanin Gene Expression in Capsicum annuum

Epistatic Interactions Influencing Anthocyanin Gene Expression in Capsicum annuum

The BANYULS gene encodes a DFR‐like protein and is a marker of early seed coat development

Quantitative Real-time Reverse Transcriptase-PCR Profiling of Anthocyanin Biosynthetic Genes during Orange Fruit Ripening

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