The Lysis of Micro-organisms by Lysozyme and Related Enzymes

Saltón, M. R. J.

doi:10.1099/00221287-18-2-481

Cited by 61 publications

(24 citation statements)

References 19 publications

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“…Other workers (e.g. Salton, 1958;Noller & Hartsell, 1961 a, b) considered that EDTA disorganized one of the layers, probably lipoprotein, which overlie and protect the mucopeptide substrate of lysozyme in the cell walls of Gram-negative bacteria (Weidel, Frank & Martin, 1960). Thus, the purified mucopeptides of these bacteria were completely dissolved by lysozyme without the addition of EDTA (Weidel et al 1960; Mandelstam, 1962).…”

Section: Introductionmentioning

confidence: 94%

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

Gray¹,

Wilkinson²

1965

Journal of General Microbiology

133

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SUMMARYA comparative survey of amino acid, amino sugar, sugar and lipid components of the cell walls of strains of Fseudomonas aeruginosa, Alcaligelzes faecalis, Escherichia coli and Proteus mirabilis was made. The cell walls of P. aeruginosa and A. faecalis, against which ethylenediaminetetra-acetic acid (EDTA) has a potent bactericidal action, differed from those of the other organisms principally in their sugar components and in their high content of phosphorus. EDTA at alkaline pH selectively solubilized a high proportion of the carbohydrate and phosphorus present, apparently as lipopolysaccharides, in the walls of sensitive organisms. It is suggested that metal cations and lipopolysaccharides in the cell walls of P. aeruginosa and A. faecalis may be essential to the structural integrity of these organisms.

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Section: Introductionmentioning

confidence: 94%

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

Gray¹,

Wilkinson²

1965

Journal of General Microbiology

133

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“…Later, its mode of action i.e. the enzymatic destruction of the bacterial cell wall was determined by Salton (1958). Antimicrobial peptides were first documented in prokaryotic cells.…”

Section: History Of Antimicrobial Peptidementioning

confidence: 99%

Antimicrobial peptides as natural bio-preservative to enhance the shelf-life of food

et al. 2016

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Antimicrobial peptides (AMPs) are diverse group of natural proteins present in animals, plants, insects and bacteria. These peptides are responsible for defense of host from pathogenic organisms. Chemical, enzymatic and recombinant techniques are used for the synthesis of antimicrobial peptides. These peptides have been found to be an alternative to the chemical preservatives. Currently, nisin is the only antimicrobial peptide, which is widely utilized in the preservation of food. Antimicrobial peptides can be used alone or in combination with other antimicrobial, essential oils and polymeric nanoparticles to enhance the shelf-life of food. This review presents an overview on different types of antimicrobial peptides, purification techniques, mode of action and application in food preservation.

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“…Significantly, the cell walls of neither the mutant strain nor Salmonella bethesda showed any comparable decrease in these amino acids. I n view of the known importance of these amino acids in the cell wall mucopeptides of other Gram-negative bacteria (Salton, 1958(Salton, , 1960Brown, 1958) it seems reasonable to presume that the autolysin present in the cultures of the parent strain removed part of the rigid mucopeptide layer from the Pseudomonas aeruginosa cell walls so that cell lysis resulted. The removal of at least 50% of the muramic acid and glucosamine from the aged Composition of ageing cell walls P. aeruginosa NCTC 6750 cell walls was taken as confirmation that the entire mucopeptide moiety was affected.…”

Section: Autolysis Of Washed Pseudornonas Aeruginosa Cell Wallsmentioning

confidence: 99%

“…Lysis of several species of sporing bacilli has been examined in some detail (Strange, 1959) and a bacterial lysozyme has been shown to be involved in some instances (Richmond, 1959). The cell walls of Gram-negative bacilli are more complex in nature than those of the Gram-positive bacteria (Salton, 1958(Salton, , 1960 and in general, lysozyme treatment of Gramnegative bacteria does not result in cell lysis except under special conditions (Warren, Gray R: Bartell, 1955;Repaslte, 1958). A number of Gram-negative bacteria undergo autolysis on ageing, but for the most part little information is available on the mode of action of the enzymes involved.…”

Section: Introductionmentioning

confidence: 99%

Composition of Cell Walls of Ageing Pseudomonas aeruginosa and Salmonella bethesda

Collins

1964

Journal of General Microbiology

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SUMMARYAnaerobic cultures of Pseudomonas aeruginosa died rapidly in the absence of nitrate and death was normally followed by extensive autolysis. A mutant which did not undergo extensive autolysis was isolated. Anaerobic cultures of Salmonella bethesda did not die or lyse even after prolonged incubation. The protein and lipid content of the parent P . aeruginosa cell walls altered during ageing in contrast to S . bethesda walls which did not alter greatly as the organisms aged. The amino acid and amino sugar content of the three strains was determined. The diaminopimelic acid, glycine, alanine, glutamic acid, glucosamine, muramic acid and glucose content of the parent cell walls decreased by 50 yo as the organisms aged.

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The Lysis of Micro-organisms by Lysozyme and Related Enzymes

Cited by 61 publications

References 19 publications

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

The Effect of Ethylenediaminetetra-acetic Acid on the Cell Walls of Some Gram-Negative Bacteria

Antimicrobial peptides as natural bio-preservative to enhance the shelf-life of food

Composition of Cell Walls of Ageing Pseudomonas aeruginosa and Salmonella bethesda

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