Composition of Cell Walls of Ageing Pseudomonas aeruginosa and Salmonella bethesda

Collins, Frank M.

doi:10.1099/00221287-34-3-379

Cited by 18 publications

(5 citation statements)

References 18 publications

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“…In the older organisms lysis however appeared not be be accompanied by such changes in the rigidity of the wall. A similar lysis without spheroplast formation has recently been reported by Collins (1964) and by Voss (1964). It is possible that this type of lysis may be due to a loosening of the bonding between the wall and the membrane postulated by McQuillen (1958) and Hughes (1962).…”

supporting

confidence: 48%

“…Such autolysis may be preceded by the formation of rounded osmotically sensitive forms (spheroplasts or protoplasts ; McQuillen, 1960), but often the organisms may lyse without spheroplast formation (Collins, 1964). In either case, the autolysis is associated with changes in the cell wall leading to loss of rigidity when spheroplasts are formed or a change in association of the cell wall and the permeability barrier when they are not formed (Collins, 1964). The present work is concerned with factors which control the autolysis of Clostridium spmogenes which occurred after collecting, washing and storing the organisms in various buffered media.…”

Section: Introductionmentioning

confidence: 99%

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The Autolysis of Clostridium sporogenes

Galli¹,

Hughes²

1965

Journal of General Microbiology

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SUMMARYWashed Clostridium sporogenes lysed during storage at 2 ' or on incubation a t 37' in various buffered salt solutions. The lysis was accompanied by a marked increase in adenosine triphosphatase activity (ATPase), due to an enzyme bound to the cytoplasmic membrane. The lysis in buffered salt solutions was most rapid in young vegetative organisms and negligible in organisms from cultures incubated for 30 hr. No lysis occurred when organisms were incubated in buffered sucrose, but young organisms formed spheroplasts under these conditions. Both young and older organisms incubated in sucrose lysed when diluted in water. After lysis the residue of older organisms appeared structurally similar to cell-wall membranes prepared by crushing the organisms. Organisms incubated in EDTA or salt solutions containing sulphydryl compounds did not lyse during the incubation or on subsequent dilution with water. It is suggested that lysis was mainly due to changes in the composition or structure of the cell wall and not to alterations in the protoplasmic membrane.

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supporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

The Autolysis of Clostridium sporogenes

Galli¹,

Hughes²

1965

Journal of General Microbiology

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“…Changes in P . aeruginosa wall composition on ageing were reported (Collins, 1964) and these might contribute to the difficulty of converting stationary phase cells to spheroplasts. Gradual removal of P .…”

Section: Discussionmentioning

confidence: 99%

The Intracellular Localization of Pseudomonas aeruginosa Lectins

Glick

Garber

1983

Microbiology

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The localization of the Pseudomonas aeruginosa lectins (PA-I and PA-II) was studied using methods of osmotic shock, freezing and thawing and spheroplast formation. Very slight release of the two lectins occurred when P. aeruginosa was exposed to magnesium-osmotic shock or was frozen and thawed. Under these conditions, release of the periplasmic 5'-nucleotidase occurred, whereas no release of cytoplasmic glucose-6-phosphate dehydrogenase activity was detected. Formation of spheroplasts from P. aeruginosa by gradual removal of the bacterial envelopes revealed low lectin activity in the treatment fluids. Osmotic shock treatment of the lysozyme treated mureinoplasts resulted in low release of glucose-6-phosphate dehydrogenase and the two lectins (10-13%) and a considerable activity (38.4%) of 5'-nucleotidase. The presence of the lectins on the outer and the cytoplasmic membranes enabled intact cells and spheroplasts of P. aeruginosa to agglutinate papain-treated human erythrocytes. These results indicate that the two lectins are located mainly in the cytoplasm with small fractions on the cytoplasmic and outer membranes and in the periplasmic space.

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“…Howwer, if the complete medium was centrifuged at 1,500 g for 20-30 minutes, the supernate would support growth. P. aeruginosa cell walls are known to be sources of bath sugars and amino acids (2), and it might (be assumed that P . ovalis can supply the same constituents.…”

Section: Stock Medium 1 This Medium (mentioning

confidence: 99%

Axenic Media for Blepharisma intermedium

Smith

Giese

1967

The Journal of Protozoology

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SYNOPSIS. Blepharisma intermedium was cultured axenically. The organisms were freed of contaminating microorganisms by serial washing in a sterile salt solution. The major nutrients in the various media were: Freshly killed or lyophilized and autoclaved bacteria (Pseudomonas ovalis), yeast extract prepared from Fleischmann's baking yeast or Fleischmann's yeast autolysate, lettuce infusion or stigmasterol, 6 B‐vitamins, and phosphate buffer at 2 times 10−3 M. The cultures were kept in the dark at 25 C. Altho the 1st division after transfer into fresh media was delayed, B. intermedium divided in approximately 32 hours thereafter in most of these media.

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Composition of Cell Walls of Ageing Pseudomonas aeruginosa and Salmonella bethesda

Cited by 18 publications

References 18 publications

The Autolysis of Clostridium sporogenes

The Autolysis of Clostridium sporogenes

The Intracellular Localization of Pseudomonas aeruginosa Lectins

Axenic Media for Blepharisma intermedium

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