The lysis of isolated fish (Oncorhynchus mykiss) gill epithelial cells by surfactants

Partearroyo, M.Aránzazu; Pilling, S.J.; Jones, M.N.

doi:10.1016/0742-8413(91)90012-i

Cited by 20 publications

(14 citation statements)

References 25 publications

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“…The greater sensitivity to pesticides of larval stage as compared with the embryonic stage of amphibians has been also reported by Hall and Swineford (1980), Berrill et al (1993), Berrill, Bertram, McGillivary, Kolohon and Pauli (1994), Berrill, Coulson, McGillivary and Pauli (1998), Edginton et al (2004). Surfactants have been demonstrated to cause lysis of gill epithelial cells in the rainbow trout (Partearroyo, Pilling, & Jones, 1991), and affect the ability of the gills to maintain osmotic balance.…”

Section: Discussionmentioning

confidence: 80%

Acute toxicity and sublethal effects of the mixture glyphosate (Roundup® Active) and Cosmo-Flux®411F to anuran embryos and tadpoles of four Colombian species

Muñoz

2014

RBT

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Abstract:Glyphosate is the most widely used herbicide in the world with application in agriculture, forestry, industrial weed control, garden and aquatic environments. However, its use is highly controversial for the possible impact on not-target organisms, such as amphibians, which are vanishing at an alarming and rapid rate. Due to the high solubility in water and ionic nature, the glyphosate requires of surfactants to increase activity. In addition, for the control of coca (Erythroxylum coca) and agricultural weeds in Colombia, formulated glyphosate is mixed and sprayed with the adjuvant Cosmo-Flux ® 411F to increase the penetration and activity of the herbicide. This study evaluates the acute toxic and sublethal effects (embryonic development, tadpole body size, tadpole swimming performance) of the mixture of the formulated glyphosate Roundup ® Active and Cosmo-Flux ® 411F to anuran embryos and tadpoles of four Colombian species under 96h laboratory standard tests and microcosms, which are more similar to field conditions as they include soil, sand and macrophytes. In the laboratory, embryos and tadpoles of Engystomops pustulosus were the most tolerant (LC 50 =3 904µg a.e./L; LC 50 =2 799µg a.e./L, respectively), while embryos and tadpoles of Hypsiboas crepitans (LC 50 =2 203µg a.e./L; LC 50 =1 424µg a.e./L, respectively) were the most sensitive. R. humboldti and R. marina presented an intermediate toxicity. Embryos were significantly more tolerant to the mixture than tadpoles, which could be likely attributed to the exclusion of chemicals by the embryonic membranes and the lack of organs, such as gills, which are sensitive to surfactants. Sublethal effects were observed for the tadpole body size, but not for the embryonic development and tadpole swimming performance. In microcosms, no toxicity (LC 50 could not be estimated), or sublethal responses were observed at concentrations up to fourfold (14.76kg glyphosate a.e./ha) the highest field application rate of 3.69kg glyphosate a.e./ha. Thus, toxicity was less in the microcosms than in laboratory tests, which may be attributed to the presence of sediments and organic matter which rapidly adsorb glyphosate and surfactants such as POEA. It is concluded that the mixture of glyphosate (Roundup ® Active) and Cosmo-Flux ® 411F, as used in the field, has a negligible toxic effect to embryos and tadpoles of the species tested in this study. Rev. Biol. Trop. 63 (1): 223-233. Epub 2015 March 01.

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Section: Discussionmentioning

confidence: 80%

Acute toxicity and sublethal effects of the mixture glyphosate (Roundup® Active) and Cosmo-Flux®411F to anuran embryos and tadpoles of four Colombian species

Muñoz

2014

RBT

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“…Triton X-100 at concentrations over the critical micellar concentration induced lysis of isolated gill epithelial cells in Oncorhynchus mykiss (103); however, Triton X-100 showed a lower effect than ionic surfactants (104). Emulgen 913 (polyoxyethylene glycol nonylphenyl ether) significantly decreased the concentration of metal-binding proteins in the hepatopancreas and lessened the heme-oxygenase activity in the kidney of red carp (105).…”

mentioning

confidence: 99%

Alkyl Ethoxylated and Alkylphenol Ethoxylated Nonionic Surfactants: Interaction with Bioactive Compounds and Biological Effects

Cserháti¹

1995

Environ Health Perspect

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“…Some authors have reported that non-ionic surfactants interact with both the lipid bilayer membrane and membrane proteins, leading to reduced oxygen transport across the membrane (Cserháti, 1995;Lindgren et al, 1996;Pärt et al, 1985). Partearroyo et al (1991) showed that surfactant caused lysis of the gill epithelial cells of Oncorhynchus mykiss leading to asphyxiation or loss of osmotic stability. The genotoxicity of Roundup ® and adjuvant alone have also been demonstrated in blood cells of Anguilla anguilla exposed for one or three days to Roundup ® and POEA (Guilherme et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Effects of a POEA surfactant system (Genamin T-200<sup>®</sup>) on two life stages of the Pacific oyster, <i>Crassostrea gigas</i>

2014

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-Surfactants used in herbicide formulations are generally considered inert with no toxic effects on animals. Polyethoxylated tallow amines (POEAs) are non-ionic surfactants used in many herbicide formulations to promote the penetration of the active matter into plant cuticles. The present study aimed to assess the toxicity of a POEA surfactant system, the Genamin T-200 ® , on two larval stages of the Pacific oyster, Crassostrea gigas. The embryotoxicity of Genamin T-200 ® was quantified after 36 hr of exposure, considering both arrested development and abnormalities in D-shaped larvae. The ability of pediveliger larvae to metamorphose was studied after 24 hr exposure to Genamin T-200 ® . According to the European toxicity classification, the present results suggest that Genamin T-200 ® could be considered very toxic to embryo larval development, with an EC 50 of 262 μg/l, and toxic to metamorphosis processes with an EC 50 of 3,027 μg/l. The high toxicity of glyphosate-based formulations compared to the active ingredient and its by-product appears to be due primarily to surfactants.

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The lysis of isolated fish (Oncorhynchus mykiss) gill epithelial cells by surfactants

Cited by 20 publications

References 25 publications

Acute toxicity and sublethal effects of the mixture glyphosate (Roundup® Active) and Cosmo-Flux®411F to anuran embryos and tadpoles of four Colombian species

Acute toxicity and sublethal effects of the mixture glyphosate (Roundup® Active) and Cosmo-Flux®411F to anuran embryos and tadpoles of four Colombian species

Alkyl Ethoxylated and Alkylphenol Ethoxylated Nonionic Surfactants: Interaction with Bioactive Compounds and Biological Effects

Effects of a POEA surfactant system (Genamin T-200<sup>®</sup>) on two life stages of the Pacific oyster, <i>Crassostrea gigas</i>

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