The Lymphoid Tissue and Antibody Formation

Murphy, James B.; Sturm, Ernest

doi:10.3181/00379727-66-16072

Cited by 26 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of adrenalectomy on antibody titres has been studied and has been shown to be associated with an elevation (1-3), a depression (4), and no change (5)(6)(7)(8). The effect of ACTH or cortisone on antibody levels in the acute experiment has resulted in an elevation (1,5,(9)(10)(11), no change (8,12), and a depression (13). The effect of ACTH or cortisone on antibody levels in the chronic experiment has resulted in an elevation (9), a depression (14,15) and no change (16,17).…”

mentioning

confidence: 99%

Quantitative Antibody Studies in Man. I. The Effect of Adrenal Insufficiency and of Cortisone on the Level of Circulating Antibodies 1

Larson¹,

Tomlinson²

1951

J. Clin. Invest.

View full text Add to dashboard Cite

Investigators have for many years been interested in the relation of the adrenal gland to antibody formation. Observations made in animals have frequently led to conflicting results. The effect of adrenalectomy on antibody titres has been studied and has been shown to be associated with an elevation (1-3), a depression (4), and no change (5-8). The effect of ACTH or cortisone on antibody levels in the acute experiment has resulted in an elevation (1, 5, 9-11), no change (8,12), and a depression (13). The effect of ACTH or cortisone on antibody levels in the chronic experiment has resulted in an elevation (9), a depression (14, 15) and no change (16,17 Individuals used in this study were from the hospital and clinic population of the Presbyterian Hospital. The patients used as controls were diagnosed as having peptic ulcer or arteriosclerotic heart disease. The five patients with adrenal insufficiency had the characteristic clinical and laboratory findings of Addison's disease. Desoxycorticosterone acetate was administered to four of the five patients during the test period. Although there is little evidence available in the literature, it has been shown that in animals DCA does not appear to influence antibody titres (10). The cortisone treated group were patients with rheumatoid arthritis receiving 200 milligrams of the drug by mouth three times each week. Cortisone treatment was started at least two weeks before immunization and was continued throughout the test period. No other medications were given. As an additional control group, eight patients with untreated rheumatoid arthritis were included in this study.8 Following a preliminary bleeding, each of the subjects was given 0.08 milligrams each of types I and II pneumococcus polysaccharides subcutaneously. Post-immunization bleedings were obtained at two and four weeks. RESULTSAs can be seen from Table I, the 12 normal individuals exhibited responses similar to those observed by Heidelberger and associates (25, 26).C-antibody was present in all sera tested and there was no rise in the titres of C-antibody in spite of sizeable changes in antibody to the type-specific capsular polysaccharides. Pre-immunization antipolysaccharide titres were low or not detectable. Maximum antibody titres were usually achieved by two weeks after immunization. A good response to one antigen was not necessarily accompanied by a similar response to the other antigen.

show abstract

mentioning

confidence: 99%

Quantitative Antibody Studies in Man. I. The Effect of Adrenal Insufficiency and of Cortisone on the Level of Circulating Antibodies 1

Larson¹,

Tomlinson²

1951

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…For several receptor-ligand pairs it is well established that structures which are in direct physical contact accumulate mutations at a significantly slower rate than structures not involved in interactions. Thus, the region of the insulin molecule interacting with the insulin receptor has been under stricter evolutionary constraints than other portions of the molecule (30). With this notion in mind it is very intriguing to note that all .class I antigen heavy chains and all class II antigen B-chains sequenced so far contain an invariant hexapeptide (Fig.…”

Section: Similarities In Structure Between Class I and Ii Antigensmentioning

confidence: 99%

“…Moreover, among the neuroendocrine hormones that may be operative, we already have a great deal of evidence indicative of glucocorticoid hormones playing a subtle, yet important role in lymphocyte physiology. For example, glucocorticoid excess results in lymphoid hypoplasia and immunosuppression (28,29), whereas glucocorticoid deficiency results in lymphoid hypertrophy and immunopotentiation (29)(30)(31)(32)(33). It is noteworthy that among the hematopoietic cells, the suppressive effects of glucocorticoids are selective for lymphocytes.…”

Section: Possible Neuroendocrine Regulation Of Tcgf Productionmentioning

confidence: 99%

Genetics of the Immune Response

Möller¹,

Möller²

1983

View full text Add to dashboard Cite

“…This problem was initially studied in adrenalectomized animals. When such animals were subsequently injected with an antigen, enhanced antibody responses were usually observed (43)(44)(45)(46)(47). However, in other studies the response was either unchanged (48)(49)(50) or depressed (51,52).…”

Section: Hydrocortisone In Antibody Syntitesismentioning

confidence: 99%

“…The next approach was the injection of adrenal cortical extracts prior to and during antigen stimulation. Paradoxically, this was generally associated with enhanced antibody responses (53)(54)(55)45), although Eisen et al (49) found no difference in serum precipitin levels of control and extract-treated adrenalectomized rats after immunization with pneumococcal polysaccharide. In recent years ACTH, cortisone, and hydrocortisone have been widely tested and were reported by most investigators to depress the immune response (cfi reviews by Mirick, reference 40, Fagraeus, reference 56, and Ward and Johnson, reference 57).…”

Section: Hydrocortisone In Antibody Syntitesismentioning

confidence: 99%

The Requirement for Hydrocortisone in Antibody-Forming Tissue Cultivated in Serum-Free Medium

Ambrose

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

It was previously reported from this laboratory that the secondary antibody response can regularly be elicited in vitro from fragments of rabbit lymph node node cultured in Eagle's medium supplemented with normal rabbit serum. Evidence is now presented that physiological levels of hydrocortisone (0.01 to 1.0 µM) can substitute for serum in the culture medium. However, with the omission of serum, serine (0.1 mM) must be included among Eagle's "essential" amino acids for consistent optimal antibody production. In some experiments the addition of insulin (0.5 unit/ml) and vitamin B12 (0.5 µg/ml) has further enhanced the secondary response in this serum-free medium.

show abstract

The Lymphoid Tissue and Antibody Formation

Cited by 26 publications

References 0 publications

Quantitative Antibody Studies in Man. I. The Effect of Adrenal Insufficiency and of Cortisone on the Level of Circulating Antibodies 1

Quantitative Antibody Studies in Man. I. The Effect of Adrenal Insufficiency and of Cortisone on the Level of Circulating Antibodies 1

Genetics of the Immune Response

The Requirement for Hydrocortisone in Antibody-Forming Tissue Cultivated in Serum-Free Medium

Contact Info

Product

Resources

About