The LOX-1 3′UTR188CT polymorphism and coronary artery disease in Turkish patients

Abstract

In coronary artery disease (CAD), a potentially reversible factor leading to cardiac death is left ventricular hypertrophy (LVH). The 3'untranslated region (3'UTR) 188CT polymorphism of lectin-like oxidized low-density lipoproteins receptor-1 (LOX-1) gene has been associated with an increased risk for CAD. We aim to investigate, in a Turkish population, whether 3'UTR188CT variation could affect the development of LVH in CAD patients. In a population-based case-control study, we compared 83 cases with CAD and 99 healthy controls for this polymorphism. The LOX-1 3'UTR188CT genotypes were determined by PCR-RFLP technique. LOX-1 3'UTR188 TT genotype was associated with significantly increased systolic blood pressure (P = 0.047) and risk of LVH (P = 0.014, OR: 3.541) when compared with the C allele carriers. In addition, the TT genotype was positively associated with decreased levels of HDL-cholesterol in the control subjects (P = 0.031) and increased levels of VLDL-C in the patient group (P = 0.009). The LOX-1 3'UTR188CT gene polymorphism may predispose to the development of LVH in CAD patients, dependent on blood pressure.

Are IVS4 SNPs of OLR1 gene associated with coronary artery disease: Is there a linkage between IVS4 SNPs?

“…10,11 In our previous study, 3'UTR 188TT genotype was associated with increased SBP levels in patients with CAD. 1 Moreover, we found that the SBP levels were statistically higher in the normal IVS4-73C allele carriers (CT+CC) than in the rare homozygote IVS4-73TT genotype carriers (115.83 ±19.76 vs 127.17 ±30.09; p = 0.045) The results are shown as mean ±standard deviation. TC -total cholesterol; TG -triglyceride; HDL-C -high-density lipoprotein cholesterol; LDL-C -lowdensity lipoprotein cholesterol; VLDL-C -very low-density lipoprotein cholesterol; BMI -body mass index; SBP -systolic blood pressure; DBP -diastolic blood pressure.…”

“…Elevated plasma and tissue levels of oxidized low-density lipoprotein (ox-LDL), as well as traditional risk factors, including age, sex, diabetes mellitus, hypercholesterolemia, high blood pressure, obesity, and smoking, were shown to have contributory effects on the development of atherosclerotic lesions. 1,2 Under some pathological conditions, such as acute myocardial infarction (AMI) and coronary artery disease (CAD), increased levels of ox-LDL have been reported. Ox-LDL, which also regulates LOX-1 activity, shows pro-atherogenic effects through oxidized low density lipoprotein (lectin-like) receptor 1 (OLR1, LOX-1).…”

LOX-1 gene variants and maternal levels of plasma oxidized LDL and malondialdehyde in patients with gestational diabetes mellitus

“…501C/C) was significantly higher in the patients with myocardial infarction than in the control group [21]. However, Kurnaz et al found that the LOX-1 3 0 UTR188C/T polymorphism was not significantly different between controls and coronary artery disease patients in Turkish population [8] and they have also shown that the frequency of the LOX-1K167N K allele was high and of the N allele was low in the coronary artery disease patients in Turkish population [22]. They found that they were not related to plasma lipoprotein levels in patients with coronary artery disease.…”

“…Due to its location in ligand-binding domain, this polymorphism may affect the binding and uptake of ox-LDL and subsequent LOX-1 activation. Despite the evidence for a functional role of these polymorphisms, results from epidemiological studies are controversial [7][8][9][10][11]. It has been suggested that the alterations of the expression of various inflammatory genes and oxidative stress markers were related with several nucleotide variants in the LOX-1 gene.…”

Needles in the genetic haystack of lipid disorders: single nucleotide polymorphisms in the microRNA regulome

“…Numerous candidate gene association studies have identifi ed SNPs within annotated 3 ′ -UTRs that may be associated with lipid-related phenotypes (70)(71)(72)(73)(74)(75)(76)(77). One interesting 2008 study of plasma cholesterol levels in ‫ف‬ 10,000 Caucasians and ‫ف‬ 3,500 African Americans found signifi cant population-specifi c interaction effects between dietary fatty acid intake and two SNPs (rs6008259 and rs3892755) in the 3 ′ -UTR of peroxisome proliferator-activated receptor ␣ Fig.…”