The longer the worse: a combined proteomic and targeted study of the long-term<i>versus</i>short-term effects of silver nanoparticles on macrophages

Dalzon, Bastien; Aude-Garcia, Catherine; Diemer, Hélène; Bons, Joanna; Marie-Desvergne, Caroline; Pérard, Julien; Dubosson, Muriel; Collin-Faure, Véronique; Carapito, Christine; Cianférani, Sarah; Carrière, Marie; Rabilloud, Thierry

doi:10.1039/c9en01329f

Cited by 14 publications

(22 citation statements)

References 70 publications

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“…A moderate toxicity was observed at lower AgNP concentrations (20% cell death at approximately 10 µg/mL Ag concentration, as reported in Table 4). Such a curvilinear response curve, with no toxicity observed at low doses and a relatively sudden onset of toxicity, has been observed on the same cell type with free AgNPs [67,68]. (48,96,120, 168 h) of inhibition halos of CNC/AgNP hybrids at two different initial AgNP contents (8.7 and 24.7 wt% Ag) with and without H2O2 redox post-treatment.…”

Section: Impact Of the Variation Of Agnp Content On Macrophage Viability And Functionsupporting

confidence: 58%

“…The latter hypothesis is favored in the literature [35] and it is rooted in from the fact that nanomaterials are confined in the lysosomes in animal cells, and thus cannot exert general cellular toxicity, and in the fact that intracellular AgNP dissolution was documented [73]. In line with these observations, intracellular release of Ag + ions from AgNPs was observed in the same cell type (macrophages) here used for toxicological testing [67,68]. Moreover, studies on the same macrophage cells with silver ion showed an EC20 at 1.6 µg/mL [67], i.e., close to the EC20 close to 4-5 µg/mL shown here with the hybrids with small nanoparticles.…”

Section: Impact Of the Variation Of Agnp Content On Macrophage Viability And Functionmentioning

confidence: 69%

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Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

et al. 2021

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There is a demand for nanoparticles that are environmentally acceptable, but simultaneously efficient and low cost. We prepared silver nanoparticles (AgNPs) grafted on a native bio-based substrate (cellulose nanocrystals, CNCs) with high biocidal activity and no toxicological impact. AgNPs of 10 nm are nucleated on CNCs in aqueous suspension with content from 0.4 to 24.7 wt%. XANES experiments show that varying the NaBH4/AgNO3 molar ratio affects the AgNP oxidation state, while maintaining an fcc structure. AgNPs transition from 10 nm spherical NPs to 300 nm triangular-shaped AgNPrisms induced by H2O2 post-treatment. The 48 h biocidal activity of the hybrid tested on B. Subtilis is intensified with the increase of AgNP content irrespective of the Ag+/Ag0 ratio in AgNPs, while the AgNSphere−AgNPrism transition induces a significant reduction of biocidal activity. A very low minimum inhibitory concentration of 0.016 mg AgNP/mL is determined. A new long-term biocidal activity test (up to 168 h) proved efficiency favorable to the smaller AgNPs. Finally, it is shown that AgNPs have no impact on the phagocytic capacity of mammalian cells.

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Section: Impact Of the Variation Of Agnp Content On Macrophage Viability And Functionsupporting

confidence: 58%

Section: Impact Of the Variation Of Agnp Content On Macrophage Viability And Functionmentioning

confidence: 69%

Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

et al. 2021

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“…Macrophages are capable of the engulfment of silver nanoparticles. 53 In vitro studies have revealed that the phagocytosis of silver nanoparticles both enhanced inflammation and damaged macrophages. 54,55 It is, therefore, possible that macrophages in chronic human wounds are also damaged by phagocytosed silver.…”

Section: Discussionmentioning

confidence: 99%

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

et al. 2021

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Although silver is an efficient antimicrobial and is a widely used antiseptic in wound healing, previous studies have reported the cytotoxic in vitro effects of silver dressings. Moreover, few studies have addressed the distribution of silver in chronic wounds. The study compares the healing of chronic wounds treated with a standardof-care silver dressing (Ag-CMC) and a dressing containing antiseptic octenidine (OCT-HA). Biopsies were taken from two wound areas before the commencement of treatment (baseline), after 2 weeks and after 6 weeks (the end of the study). We analyzed the histopathologic wound-healing score, silver distribution, and expression of selected genes. The wound-healing score improved significantly in the wounded area treated with OCT-HA after 2 weeks compared to the baseline and the Ag-CMC. The Ag-CMC wound areas improved after 6 weeks compared to the baseline. Moreover, collagen maturation and decreases in the granulocyte and macrophage counts were faster in the OCT-HA parts. Treatment with OCT-HA resulted in less wound slough.The silver, visualized via autometallography, penetrated approximately 2 mm into the wound tissue and associated around capillaries and ECM fibers, and was detected in phagocytes. The metallothionein gene expression was elevated in the Ag-CMC wound parts. This exploratory study determined the penetration of silver into human chronic wounds and changes in the distribution thereof during treatment. We observed that silver directly affects the cells in the wound and elevates the metallothionein gene expression. Octenidine and hyaluronan dressings provide a suitable alternative to silver and carboxymethyl cellulose dressings without supplying silver to the wound.

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“…Going into more detail about this system, we selected macrophages as the target cell type because they are the scavenger cell type for particulate materials, are found in numerous locations in the body, and are key players in inflammatory reactions [ 31 ]. Mouse macrophage cell lines show excellent responses to many stimuli and can be used in various exposure scenarii such as chronic exposures [ 32 ]. For such long-term experiments and for medium-term experiments such as the recovery scheme used here, the cells must be cultured in a complete culture medium containing serum because they will not survive for such extended period of times in serum-free media.…”

Section: Discussionmentioning

confidence: 99%

How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-Informed View

et al. 2020

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Synthetic amorphous silica is one of the most used nanomaterials, and numerous toxicological studies have studied its effects. Most of these studies have used an acute exposure mode to investigate the effects immediately after exposure. However, this exposure modality does not allow the investigation of the persistence of the effects, which is a crucial aspect of silica toxicology, as exemplified by crystalline silica. In this paper, we extended the investigations by studying not only the responses immediately after exposure but also after a 72 h post-exposure recovery phase. We used a pyrolytic silica as the test nanomaterial, as this variant of synthetic amorphous silica has been shown to induce a more persistent inflammation in vivo than precipitated silica. To investigate macrophage responses to pyrolytic silica, we used a combination of proteomics and targeted experiments, which allowed us to show that most of the cellular functions that were altered immediately after exposure to pyrolytic silica at a subtoxic dose, such as energy metabolism and cell morphology, returned to normal at the end of the recovery period. However, some alterations, such as the inflammatory responses and some aldehyde detoxification proteins, were persistent. At the proteomic level, other alterations, such as proteins implicated in the endosomal/lysosomal pathway, were also persistent but resulted in normal function, thus suggesting cellular adaptation.

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The longer the worse: a combined proteomic and targeted study of the long-termversusshort-term effects of silver nanoparticles on macrophages

Abstract: At equal cumulated dose, a chronic exposure to silver nanoparticles produces more effects on macrophages than an acute exposure.

Cited by 14 publications

References 70 publications

Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-Informed View

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