Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

Musino, Dafne; Devcic, Julie; Lelong, Cécile; Luche, Sylvie; Rivard, Camille; Dalzon, Bastien; Landrot, Gautier; Rabilloud, Thierry; Capron, Isabelle

doi:10.3390/nano11071862

Cited by 14 publications

(10 citation statements)

References 78 publications

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“…Chemically synthesized nanoparticles often require toxic reducing and stabilizing agents (hydrazine hydrate, sodium borohydride, DMF, and ethylene glycol) [ 12 ]. The production of nanomaterials for biomedical applications requires natural methods of synthesis involving microorganisms and biological systems.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity

et al. 2021

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Natural extracts are a rich source of biomolecules that are useful not only as antioxidant drugs or diet supplements but also as complex reagents for the biogenic synthesis of metallic nanoparticles. The natural product components can act as strong reducing and capping substrates guaranteeing the stability of formed NPs. The current work demonstrates the suitability of extracts of Camellia sinensis, Ilex paraguariensis, Salvia officinalis, Tilia cordata, Levisticum officinale, Aegopodium podagraria, Urtica dioica, Capsicum baccatum, Viscum album, and marine algae Porphyra Yezoensis for green synthesis of AgNPs. The antioxidant power of methanolic extracts was estimated at the beginning according to their free radical scavenging activity by the DPPH method and reducing power activity by CUPRAC and SNPAC (silver nanoparticle antioxidant capacity) assays. The results obtained by the CUPRAC and SNAPC methods exhibited excellent agreement (R2~0.9). The synthesized AgNPs were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), dynamic light scattering (DLS) particle size, and zeta potential. The UV-vis absorption spectra showed a peak at 423 nm confirming the presence of AgNPs. The shapes of extract-mediated AgNPs were mainly spherical, spheroid, rod-shaped, agglomerated crystalline structures. The NPs exhibited a high negative zeta potential value in the range from −49.8 mV to −56.1 mV, proving the existence of electrostatic stabilization. FTIR measurements indicated peaks corresponding to different functional groups such as carboxylic acids, alcohol, phenol, esters, ethers, aldehydes, alkanes, and proteins, which were involved in the synthesis and stabilization of AgNPs. Among the examined extracts, green tea showed the highest activity in all antioxidant tests and enabled the synthesis of the smallest nanoparticles, namely 62.51, 61.19, and 53.55 nm, depending on storage times of 30 min, 24 h, and 72 h, respectively. In turn, the Capsicum baccatum extract was distinguished by the lowest zeta potential, decreasing with storage time from −66.0 up to −88.6 mM.

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Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity

et al. 2021

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“…The synthesis method of this system was carried following the experimental protocol proposed in another work of our group. 37,39 A volume of 500 mL of CNC suspension (15 g/L) was dialyzed against water for 3 days and then mixed with 25 mL of AgNO 3 aqueous solution (1 M). Then, 40 mL of freshly-prepared NaBH 4 aqueous solution (1 M) were gently added to the CNC/AgNO 3 aqueous suspension under stirring to reduce Ag + ions and synthetize AgNPs (NaBH 4 /AgNO 3 molar ratio of 2).…”

Section: Methodsmentioning

confidence: 99%

“…Since the oxidation process occurs at the surface, this approach should preserve the full biocidal efficiency of NPs with a reduction of the necessary AgNP amount in the system. Notably, a good AgNP dispersion can be reached by grafting AgNPs on a polysaccharide such as cellulose nanocrystals (CNCs) thus obtaining a biocidal hybrid partly bio-based NP [33][34][35][36][37] In this work, we propose paint formulation strategies to reach high biocide efficiency while minimizing the risk at each stage of the life cycle, promoting a new safety-by-designed approach. The antibacterial properties are obtained by introducing well-dispersed hybrid NPs with AgNPs nucleated at surface of CNCs, in paint.…”

Section: Introductionmentioning

confidence: 99%

CNC/AgNP hybrids as safer-by-design biocides in paints

Musino

Rosset

Lelong

et al. 2021

Environ. Sci.: Nano

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“…NPs synthesized chemically frequently need harmful stabilizing and reducing chemicals (ethylene glycol, hydrazine hydrate, dimethylformamide , and sodium borohydride) [ 15 ]. Natural techniques of synthesis involving microbes and biological systems are required for the fabrication of nanomaterials for biomedical purposes [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Green Engineered Silver Nanoparticles through Urtica dioica: An Inhibition of Microbes and Alleviation of Cellular and Organismal Toxicity in Drosophila melanogaster

et al. 2022

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Plant fractions have a diversity of biomolecules that can be used to make complicated reactions for the bioactive fabrication of metal nanoparticles (NPs), in addition to being beneficial as antioxidant medications or dietary supplements. The current study shows that Urtica dioica (UD) and biologically synthesized silver nanoparticles (AgNPs) of UD have antibacterial and antioxidant properties against bacteria (Escherichia coli and Pseudomonas putida) and Drosophila melanogaster (Oregon R+). According to their ability to scavenge free radicals, DPPH, ABTS, TFC, and TPC initially estimated the antioxidant potential of UD and UD AgNPs. The fabricated AgNPs were analyzed (UV‒Vis, FTIR, EDS, and SEM) to determine the functional groups (alcohol, carboxylic acids, phenol, proteins, and aldehydes) and to observe the shape (agglomerated crystalline and rod-shaped structure). The disc diffusion method was used to test the antimicrobial properties of synthesized Ag-NPs against E. coli and P. putida. For 24 to 120 h, newly enclosed flies and third instar larvae of Drosophila were treated with UD and UD AgNPs. After exposure, tests for biochemical effects (acetylcholinesterase inhibition and protein estimation assays), cytotoxicity (dye exclusion), and behavioral effects (jumping and climbing assays) were conducted. The results showed that nanoparticles were found to have potent antimicrobial activity against all microbial strains tested at various concentrations. In this regard, ethno-medicinal characteristics exhibit a similar impact in D. melanogaster, showing (p < 0.05) significantly decreased cellular toxicity (trypan blue dye), enhanced biochemical markers (AChE efficacy and proteotoxicity), and improved behavioral patterns in the organism treated with UD AgNPs, especially in comparison to UD extract. The results of this study may help in the utilization of specific plants as reliable sources of natural antioxidants that may have been beneficial in the synthesis of metallic NPs, which aids in the production of nanomedicine and other therapeutic applications.

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Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects

Cited by 14 publications

References 78 publications

Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity

Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity

CNC/AgNP hybrids as safer-by-design biocides in paints

Synthesis of Green Engineered Silver Nanoparticles through Urtica dioica: An Inhibition of Microbes and Alleviation of Cellular and Organismal Toxicity in Drosophila melanogaster

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