The long-term effect of a partial whey hydrolysate formula on the prophylaxis of atopic disease

Vandenplas, Yvan; Hauser, Bruno; Borre, C Van den; Clybouw, C.; Mahler, Tania; Hachimi-Idrissi, Saı̈d; Deraeve, L.; Malfroot, Anne; I, Dab

doi:10.1007/bf02029362

Cited by 138 publications

(85 citation statements)

References 28 publications

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“…To date, different results with different ranges of CM protein hydrolysis have been reported in rodent models [21,25,26,27] and clinical studies [28,29,30,31]. In prevention strategies, there is not really a need to check for IgE binding, because specific IgE antibodies have not yet been produced.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Treatment of Whey Proteins in Cow’s Milk Results in Differential Inhibition of IgE-Mediated Mast Cell Activation Compared to T-Cell Activation

Knipping

Esch

Dijk

et al. 2012

Int Arch Allergy Immunol

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Background: Cow’s milk (CM) hydrolysates are frequently used as milk substitutes for children with CM allergy. In hydrolysates, allergenic epitopes within CM proteins are diminished by enzymatic treatment. The aim of this study was to examine the allergenic and immunogenic properties of whey proteins during hydrolysis. Methods: During hydrolysis, samples were obtained at 0, 10, 15, 30, 45, 60, 75 and 90 min. Degradation was checked by HPLC and SDS-PAGE. Allergenic potential was analyzed by IgE crosslinking capacity of human Fcε receptor type 1-transduced rat basophilic leukemia cells sensitized with serum of CM-allergic patients. Whey-sensitized C3H/HeOuJ mice were ear challenged intracutaneously with the hydrolysates. Immunogenicity was tested using whey-specific human T-cell clones and T-cell lines at the level of proliferation and release of IL-4, IL-10, IL-13 and IFN-γ. Results: After 15 min of hydrolysis, the majority of the proteins were degraded. Hydrolysis for 15 min resulted in 92% inhibition of mast cell degranulation and in 82% reduction of ear swelling in the mouse model. In contrast, T-cell-stimulatory capacity was less affected by hydrolysis: reduction of human T-cell proliferation was only 9%. This was further reduced to 57 and 74% after 30 and 45 min of hydrolysis, respectively. Cytokine production followed the pattern of T-cell proliferation. Conclusion: Via differential analysis of allergenic versus immunogenic properties of the time kinetics of hydrolysis of whey proteins, we have demonstrated specific hydrolysis conditions with reduced IgE-crosslinking responses but retained T-cell activating properties. This approach might be useful in better defining CM hydrolysates.

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Section: Discussionmentioning

confidence: 99%

Enzymatic Treatment of Whey Proteins in Cow’s Milk Results in Differential Inhibition of IgE-Mediated Mast Cell Activation Compared to T-Cell Activation

Knipping

Esch

Dijk

et al. 2012

Int Arch Allergy Immunol

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“…Feeding dif®culties are frequently experienced by human infants who, for medical reasons such metabolic disorder or allergy, must make transitions to new and often unpleasant-tasting formulas (Vandenplas, Hauser, Van den Borre, Sacre, & Dab, 1992). We evaluated whether sucking might be enhanced, and thus diet transitions facilitated, by applying olfactory conditioning techniques similar to those that have been shown to in¯uence ingestion in developing animals.…”

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confidence: 99%

Olfactory conditioning facilitates diet transition in human infants

Coyle¹,

Arnold²,

Goldberg-Arnold³

et al. 2000

Dev. Psychobiol.

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We evaluated whether Pavlovian conditioning methods could be used to increase the ingestion of non-preferred solutions by formula-fed human infants. In baseline measures, 5±7 month old infants sucked less frequently and consumed less water than regular formula. During a 3-day olfactory conditioning period, parents placed a small scented disk, the conditioned stimulus, on the rim of their infants' formula bottle at every feeding. Following this training, infants' responses to water were tested when their water bottles had a disk scented with the training odor, a novel odor, or no odor. Infants tested with the training odor sucked more frequently and consumed signi®cantly more water than they had at baseline. Infants tested with no odor or a novel odor consumed water at or below baseline levels. These data demonstrate that olfactory conditioning can be used to enhance ingestion in infants and suggest that such methods may be useful for infants experiencing dif®culty when making transitions from one diet to another.

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“…On the other hand, pHFs are recommended for infants at the risk of developing CMA since it is believed that the larger peptides can be recognised by the immune system, giving rise to the development of tolerance in the absence of an intact allergen [6]. Clinical studies indicate that both eHF and pHF may have a primary preventive capacity [5, 7-10]. However, the evidence is not strong [11].…”

Section: Introductionmentioning

confidence: 99%

Preclinical Brown Norway Rat Models for the Assessment of Infant Formulas in the Prevention and Treatment of Cow’s Milk Allergy

Jensen

Larsen

Madsen

et al. 2019

Int Arch Allergy Immunol

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Background: Infant formulas (IFs) based on hydrolysed cow’s milk proteins are central in the management of cow’s milk allergy (CMA) in infants and small children. New IF compositions with improved prevention and treatment properties are needed, along with appropriate preclinical animal models, to evaluate these properties before introduction into humans. Objectives: We aimed to develop preclinical models for the assessment of the primary preventive and desensitising capacity of cow’s milk IF in allergy-prone, high-IgE responder Brown Norway rats. Method: Preventive capacity was assessed in cow’s milk-naïve rats given a 2- or 4-week regimen of whey-based extensively hydrolysed IF (eHF), partially hydrolysed IF (pHF), or intact β-lactoglobulin (BLG) ad libitum in drinking bottles, followed by intraperitoneal (i.p.) immunisation with BLG. Desensitising capacity was assessed in orally BLG-sensitised rats after a 3- or 6-week regimen of eHF, pHF, or intact BLG administration in drinking bottles, followed by i.p. challenge with BLG. Primary preventive and desensitising capacity were analysed by serum BLG-specific IgG1 and IgE. Results: The preventive regimens did not induce detectable BLG-specific IgG1 or IgE in cow’s milk-naïve rats. A preventive regimen consisting of pHF or BLG, but not eHF, induced complete tolerance to BLG, as demonstrated by the absence of BLG-specific IgE following i.p. immunisation. Desensitising regimens had a limited effect on BLG-specific IgG1 or IgE when comparing sensitised rats before and after treatment. Challenge with BLG (i.p.) increased BLG-specific IgE in all treatment regimens except for in the BLG group, suggesting a limited desensitising capacity of IF based on hydrolysates and a need for the presence of intact allergen for desensitisation. Conclusions: The presented models highlight that different mechanisms are at play in the induction of de novo tolerance to cow’s milk proteins and the desensitisation of CMA. Different IF products may be needed for the primary prevention and treatment of CMA.

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The long-term effect of a partial whey hydrolysate formula on the prophylaxis of atopic disease

Cited by 138 publications

References 28 publications

Enzymatic Treatment of Whey Proteins in Cow’s Milk Results in Differential Inhibition of IgE-Mediated Mast Cell Activation Compared to T-Cell Activation

Enzymatic Treatment of Whey Proteins in Cow’s Milk Results in Differential Inhibition of IgE-Mediated Mast Cell Activation Compared to T-Cell Activation

Olfactory conditioning facilitates diet transition in human infants

Preclinical Brown Norway Rat Models for the Assessment of Infant Formulas in the Prevention and Treatment of Cow’s Milk Allergy

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