The long noncoding RNA uc.294 is upregulated in early‐onset pre‐eclampsia and inhibits proliferation, invasion of trophoblast cells (HTR‐8/SVneo)

Song, Xuejing; Li, Chunyan; Li, Jingyun; Liu, Lan; Li, Meng; Ding, Hongjuan; Long, Wei

doi:10.1002/jcp.27916

Cited by 11 publications

(5 citation statements)

References 37 publications

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“… [ 184 ] Uc.294 placenta upregulated / Uc.294 inhibits proliferation, invasion and promotes apoptosis of trophoblast cells HTR-8/SVneo. [ 185 ] PSG10P placenta upregulated miR-19a-3p/IL1RAP IL1RAP promotes the expression of caspase-3 but inhibits MMP9 to suppresses proliferation, migration, and invasion of trophoblast cells. [ 186 ] 00473 placenta downregulated LSD1/TFPI2 lnc00473 inhibits the expression of tissue factor pathway inhibitor 2 (TFPI2) through binding to lysine-specific demethylase 1 (LSD1) to inhibits cell proliferation and promotes apoptosis.…”

Section: Lncrnas and Pementioning

confidence: 99%

Roles of noncoding RNAs in preeclampsia

Sun

Qin

Zhang

et al. 2021

Reprod Biol Endocrinol

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Preeclampsia (PE) is an idiopathic disease that occurs during pregnancy. It comprises multiple organ and system damage, and can seriously threaten the safety of the mother and infant throughout the perinatal period. As the pathogenesis of PE is unclear, there are few specific remedies. Currently, the only way to eliminate the clinical symptoms is to terminate the pregnancy. Although noncoding RNA (ncRNA) was once thought to be the “junk” of gene transcription, it is now known to be widely involved in pathological and physiological processes, including pregnancy-related disorders. Moreover, there is growing evidence that the unbalanced expression of specific ncRNA is involved in the pathogenesis of PE. In the present review, we summarize the expression patterns of ncRNAs, i.e., microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), and the functional mechanisms by which they affect the development of PE, and examine the clinical significance of ncRNAs as biomarkers for the diagnosis of PE. We also discuss the contributions made by genetic polymorphisms and epigenetic ncRNA regulation to PE. In the present review, we wish to explore and reinforce the clinical value of ncRNAs as noninvasive biomarkers of PE.

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Section: Lncrnas and Pementioning

confidence: 99%

Roles of noncoding RNAs in preeclampsia

Sun

Qin

Zhang

et al. 2021

Reprod Biol Endocrinol

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“…Transwell invasion assay. Transwell assays were carried out as described previously (19). Cells (2x10 4 cells/well) were cultured at 37˚C for 24 h in 24-well plates with Transwell inserts precoated with Matrigel ® (37˚C for 30 min; BD Pharmingen; BD Biosciences).…”

Section: Luciferase Reporter Assaymentioning

confidence: 99%

MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

et al. 2021

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Preeclampsia is a pregnancy disorder that is primarily associated with maternal and neonatal or fetal morbidity and mortality. The discovery of dysregulated microRNAs (miRs) and their roles in preeclampsia has provided new insight into the mechanisms involved in pregnancy-related disorders. In the present study, quantitative PCR demonstrated that the expression levels of miR-524-5p were lower in patients with preeclampsia than those in normal pregnant women. Cell Counting Kit-8 and Transwell assays indicated that overexpression of miR-524-5p promoted the proliferation and invasion of HTR-8/SVneo cells, whereas inhibition of miR-524-5p suppressed HTR-8/SVneo cell proliferation and invasion. Furthermore, NUMB endocytic adaptor protein (NUMB), a negative regulator of the Notch signaling pathway and a target gene of miR-524-5p, limited the effects of miR-524-5p on HTR-8/SVneo cell invasion and migration. The present study demonstrated that miR-524-5p regulated the proliferation and invasion of HTR-8/SVneo cells at least partly by targeting NUMB to regulate the Notch signaling pathway.

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“…14 LncRNA SND1-IT1 can up-regulate POU2F1 to accelerate osteosarcoma proliferation and migration by sponging miR-665. 15 Studies have shown that lncRNAs, such as uc.294, 16 NR_002794, 17 CRNDE, 18 and TUG1, 19 are involved in PE development by affecting trophoblast functions. In the previous study, we detected lncRNAs specifically expressed in the placenta tissues of PE patients by using microarray assays.…”

Section: Introductionmentioning

confidence: 99%

LncRNA LINC00534 regulates cell proliferation and migration via the miR‐494‐3p/PTEN axis in HTR‐8/SVneo cells

Song

Zhang

Xia

et al. 2022

Clinical Laboratory Analysis

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Background LncRNA LINC00534 has been found to be differentially expressed in placental tissue samples of preeclampsia (PE), but the exact mechanism is still unclear. Methods In vitro assays were carried out in HTR‐8/SVneo cells using various methods, including cell counting kit‐8 (CCK‐8), transwells, flow cytometry, and Western blotting (WB) and quantitative polymerase chain reaction. RNA pull‐down and bioinformatics analysis were applied to examine other potential underlying mechanisms involved. Results We found that there was a high expression of LINC00534 in the placental tissues of patients with PE. LINC00534 overexpression (OE) significantly inhibited cell proliferation and migration as well as accelerated cell apoptosis in HTR8/SVneo cells. The knockdown of LINC00534 produced an opposite trend. Mechanistically, LINC00534 promoted the expressions of PTEN (Phosphatase and tensin homolog) through decreasing miR‐494‐3p. Further rescue studies showed that LINC00534 played a role by targeting mir‐494‐3p, which controlled the growth and migration of HTR‐8/SVneo trophoblast cells via regulating PTEN/PI3K/AKT (Phosphatidylinositol3‐kinase/protein kinase B). Moreover, lncRNA pull‐down assay identified 198 potential bound proteins for LINC00534. Those proteins were mostly involved in RNA processing and modification, posttranslational modification, protein turnover, and chaperones. Conclusion Overall, by suppressing HTR8/SVneo cell growth and migration via the miR‐494‐3p/PTEN axis and other mechanisms, LINC00534 offers new insight into PE pathogenesis.

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The long noncoding RNA uc.294 is upregulated in early‐onset pre‐eclampsia and inhibits proliferation, invasion of trophoblast cells (HTR‐8/SVneo)

Cited by 11 publications

References 37 publications

Roles of noncoding RNAs in preeclampsia

Roles of noncoding RNAs in preeclampsia

MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

LncRNA LINC00534 regulates cell proliferation and migration via the miR‐494‐3p/PTEN axis in HTR‐8/SVneo cells

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