Roles of noncoding RNAs in preeclampsia

Sun, Ning; Qin, Shiting; Zhang, Lu; Liu, Shiguo

doi:10.1186/s12958-021-00783-4

Cited by 29 publications

(24 citation statements)

References 204 publications

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“…Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species (small nuclear RNA, small nucleolar RNAs, telomerase RNA, tRNA-derived fragments, tRNA halves, microRNA, small interfering RNA, piwi-interacting RNA, enhancer RNA, long non-coding RNA, and circular RNA), of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…A growing body of evidence supports the involvement of placenta expressed miRNAs and lncRNAs in the regulation of crucial processes of placenta development and function. The complete biological roles of the most miRNAs and lncRNA in placenta remain scarce, nevertheless, it has been unequivocally shown that altered expression of numerous miRNAs as well as lncRNAs in placenta, is related to pathophysiological processes that occur in PE [ 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

Munjas

Sopić

Stefanović

et al. 2021

IJMS

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Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

Munjas

Sopić

Stefanović

et al. 2021

IJMS

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“…It has been reported that circRNAs are significantly involved in regulating cell proliferation, apoptosis, and differentiation ( Jia and Li, 2019 ; Chen et al, 2021 ; Gao et al, 2021 ; Sun et al, 2021 ). High levels of circRNA_33186 have been reported to promote apoptosis and inhibit proliferation in osteoarthritis ( Zhou et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The Circ-CYP24A1-miR-224-PRLR Axis Impairs Cell Proliferation and Apoptosis in Recurrent Miscarriage

Gao

et al. 2022

Front. Physiol.

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AimRecurrent miscarriage (RM) is associated with numerous clinical factors. However, some RM occurred without specific factors. It has been revealed that some molecules such as hormones, miRNAs, and transcription factors are involved in RM by regulating proliferation, apoptosis, etc. However, the mechanism of RM has yet to be identified clearly. Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs that often act as sponges for miRNAs or binds to proteins involved in biological processes. However, the functional role of circRNAs in the uterine decidua of patients with early RM is still unclear. In this study, we aimed to investigate the mechanisms of circ-CYP24A1 in RM.MethodsThe Dual-Luciferase Activity Assay was designed to analyze the bonding between circ-CYP24A1 and miR-224, and miR-224 and prolactin receptor (PRLR) mRNA 3′UTR. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to observe the expression of circ-CYP24A1 and PRLR in the decidua. Rescue experiments were performed to investigate the regulating effects of circ-CYP24A1, miR-224, and PRLR. Western blotting was conducted to test the expression level of PRLR. The proliferation and apoptosis-related markers in Ishikawa cells were analyzed using CCK8, immunofluorescence staining, and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay.ResultsIn this study, based on the microarray analysis data, we identified a high level of circ-CYP24A1 and PRLR in the decidua of patients with early RM. Based on the bioinformatics prediction, the binding relationship between circ-CYP24A1 and miR-224, as well as miR-224 and PRLR, were verified. Functional experiments demonstrated that circ-CYP24A1 regulated proliferation and apoptosis by binding to and inhibiting miR-224, resulting in increased PRLR expression. Taken together, this study provides new insights into the mechanism of RM.ConclusionIn this study, we found that circ-CYP24A1 plays a role in RM by impairing the balance of cell proliferation and apoptosis by sponging miR-224, thereby regulating PRLR.

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“…Several miRNAs are specific to placental pathologies; for example, hsa-miR-210, which is influenced by the expression of the HIF-1⍺ hypoxia response gene, shows increased expression in preeclamptic placentas when compared to controls (A. Chen et al, 2021 ; Sun et al, 2021 ; Frazier et al, 2020 ). Moreover, recent studies have revealed miRNA isoforms (isomiRs) that have variations in their mature miRNA sequences, which further expands the repertoire of mRNAs targeted for regulation.…”

Section: Sncrna Expression Impacts Placental Functionmentioning

confidence: 99%

Small Non-Coding RNAs in the Human Placenta: Regulatory Roles and Clinical Utility

Telkar

Stewart²,

Pewarchuk³

et al. 2022

Front. Genet.

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The placenta is a vital organ formed during pregnancy, and being the interface between the mother and fetus, it is paramount that placental functioning is strictly controlled. Gene expression in the placenta is finely tuned—with aberrant expression causing placental pathologies and inducing stress on both mother and fetus. Gene regulation is brought upon by several mechanisms, and small non-coding RNAs (sncRNAs) have recently been appreciated for their contribution in gene repression. Their dysregulation has been implicated in a range of somatic and inherited disorders, highlighting their importance in maintaining healthy organ function. Their specific roles within the placenta, however, are not well understood, and require further exploration. To this end, we summarize the mechanisms of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), and transfer RNAs (tRNAs), their known contributions to human placental health and disease, the relevance of sncRNAs as promising biomarkers throughout pregnancy, and the current challenges faced by placental sncRNA studies.

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Roles of noncoding RNAs in preeclampsia

Cited by 29 publications

References 204 publications

Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

The Circ-CYP24A1-miR-224-PRLR Axis Impairs Cell Proliferation and Apoptosis in Recurrent Miscarriage

Small Non-Coding RNAs in the Human Placenta: Regulatory Roles and Clinical Utility

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