The Long Noncoding RNA HOTAIR Promotes Colorectal Cancer Progression by Sponging miR-197

Lu, Xinyang; Liu, Zhiqiang; Ning, Xiaofei; Huang, Lunhua; Jiang, Biwang

doi:10.3727/096504017x15105708598531

Cited by 37 publications

(29 citation statements)

References 34 publications

(18 reference statements)

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“…For example, it has been reported that HOTAIR can mediate CRC tumorigenesis and liver metastasis in vivo (26). Furthermore, HOTAIR could promote CRC cell proliferation, migration, and invasion via sponging miR-197 (27). Consistent with a previous study, high HOTAIR expression indicates poor prognosis for patients with CRC metastasis (28).…”

Section: Discussionsupporting

confidence: 72%

A Robust 6-lncRNA Prognostic Signature for Predicting the Prognosis of Patients With Colorectal Cancer Metastasis

Chen

Wang

et al. 2020

Front. Med.

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Objective: Our study aimed to construct a robust long non-coding RNA (lncRNA) prognostic signature for colorectal cancer (CRC) metastasis. Methods: Differentially expressed lncRNAs were identified between metastatic CRC and non-metastatic CRC samples from The Cancer Genome Atlas Database (TCGA) using the edgeR package. The differentially expressed lncRNAs with prognosis of patients with CRC metastasis were identified by univariate Cox regression analysis, followed by a stepwise multivariate Cox regression model. The survminer package in R was used to identify the optimal cutoff point for high-risk and low-risk groups. The receiver operating characteristic (ROC) curves were plotted to assess this signature. To explore potential signaling pathways associated with these lncRNAs, Gene Set Enrichment Analysis (GSEA) was performed. Results: A 6-lncRNA signature was built based on the lncRNA expression profile for CRC metastasis. The optimal cutoff value was used to classify high-risk and low-risk groups using the survminer package. The high-risk groups could have poorer survival time than the low-risk groups. ROC curve result indicated that this lncRNA signature had high sensitivity and accuracy. GSEA analysis results showed that the six lncRNAs were significantly enriched in several CRC metastasis-related signaling pathways such as "cell cycle," "DNA replication," "mismatch repair," "oxidative phosphorylation," "regulation of autophagy," and "insulin signaling pathway." Conclusion: Our study constructed a 6-lncRNA model for predicting the survival outcomes of patients with CRC metastasis, which could become potential prognostic biomarkers, and therapeutic targets for CRC metastasis.

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Section: Discussionsupporting

confidence: 72%

A Robust 6-lncRNA Prognostic Signature for Predicting the Prognosis of Patients With Colorectal Cancer Metastasis

Chen

Wang

et al. 2020

Front. Med.

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“…The elevated expression of Hotair was observed in multiple cancer types, [16][17][18] and our results also demonstrated that it was significantly upregulated in a panel of CRC cells ( Figure 1A). In 20 paired CRC clinical specimens, the expression of Hotair was increased in CRC tissues when compared with their adjacent nontumor tissues ( Figure 1B).…”

Section: Hotair Was Significantly Upregulated In Crc Cells and Clinsupporting

confidence: 76%

Hotair mediates tumorigenesis through recruiting EZH2 in colorectal cancer

Huang

Zhang

Zhu

et al. 2018

J of Cellular Biochemistry

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Long noncoding RNAs (lncRNA) have been demonstrated to extensively participate in a wide spectrum of biological activities ranging from embryogenesis and cancer progression. HOX transcript antisense RNA (Hotair), an lncRNA located in the HOXC locus, has been reported to play an important role in carcinogenesis. As a well‐known oncogene, it potentiates cancer metastasis and tumor progression. And it also serves as a biomarker for poor prognosis and tumor recurrence. In this study, Hotair was found to be upregulated in colorectal cancer (CRC) cells and clinical specimens. Further investigation showed that knockdown of Hotair dramatically suppressed cell proliferation and colony formation, suggesting that Hotair may stimulate tumorigenesis of CRC. The enhancer of zeste homolog 2 (EZH2), a regulator of epigenetic modification, was upregulated in CRC cells and clinical samples. And the silence of EZH2 significantly suppressed cell viability and colony formation. Furthermore, the RNA immunoprecipitation assay revealed that Hotair directly bound EZH2 in CRC cells. In conclusion, Hotair mediated tumorigenesis via recruiting EZH2, which might shed light on the development of a novel therapeutic approach for patients with CRC.

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“…The abnormal expression of lncRNA plays an important role in cancer progression. Recently, large amount of researches have demonstrated that many lncRNAs such as HOTAIR, and metastasis associated lung adenocarcinoma transcript 1 (MALAT1) are highly expressed in colorectal cancer, breast cancer, hepatocellular carcinoma and some other cancer tissues and are closely related to a variety of cancer metastasis, thus can be used as targets for cancer early diagnosis, treatment and prognosis prediction [17,18]. CCAT2, as a novel oncogene, is located in the hotspot of tumour-related rs6983267 SNP, which promotes the occurrence and development of kinds of cancers by affecting Wnt and TGF-b signalling pathways [9,19].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA CCAT2 acts as an oncogene in osteosarcoma through regulation of miR-200b/VEGF

Liu

Kong

Sun

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background/aim: Colon cancer-associated transcript 2 (CCAT2) is a new lncRNA, which is closely associated with risk of several cancers. The aim of this study was to explore the regulatory mechanism of CCAT2 in osteosarcoma (OSA). Methods: Cells were transfected with si-CCAT2, microRNA (miR)-200b inhibitor and the corresponding controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the expression of CCAT2 and miR-200b in OSA tissues and cell lines. CCK8 and bromodeoxyuridine (BrdU) were conducted to examine cell proliferation. Apoptosis was detected by PI/FITC-Annexin V combining with flow cytometric analysis. Migration and invasion were respectively measured through transwell chambers assays. Western blot was used to examine expressions of relative proteins. Results: CCAT2 was highly expressed and miR-200b was lowly expressed in OSA tissues and cell lines. Knockdown of CCAT2 suppressed cell proliferation, migration and invasion but induced apoptosis and up-regulation of miR-200b. miR-200b inhibitor weakened the effect of si-CCAT2 on cell progression and cell mobility. Besides, knockdown of CCAT2 blocked the PI3K/Akt and AMPK pathways through upregulating miR-200b. Conclusions: The CCAT2/miR-200b/vascular endothelial growth factor (VEGF) axis plays important regulating effect in OSA through the PI3K/Akt and AMPK pathways.

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The Long Noncoding RNA HOTAIR Promotes Colorectal Cancer Progression by Sponging miR-197

Cited by 37 publications

References 34 publications

A Robust 6-lncRNA Prognostic Signature for Predicting the Prognosis of Patients With Colorectal Cancer Metastasis

A Robust 6-lncRNA Prognostic Signature for Predicting the Prognosis of Patients With Colorectal Cancer Metastasis

Hotair mediates tumorigenesis through recruiting EZH2 in colorectal cancer

RETRACTED ARTICLE: Long non-coding RNA CCAT2 acts as an oncogene in osteosarcoma through regulation of miR-200b/VEGF

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