The long noncoding RNA CASC2 functions as a competing endogenous RNA by sponging miR-18a in colorectal cancer

Huang, Guiqian; Wu, Xiaoli; Shi, Li; Xu, Xiaoqun; Zhu, Hua; Chen, Xiangjian

doi:10.1038/srep26524

Cited by 128 publications

(126 citation statements)

References 42 publications

(45 reference statements)

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…An example of this type of regulation was exemplified by cancer susceptibility candidate 2 (CASC2), whose decreased expression is in part due to its oncogenic effects on the expression and activity of miR-18a in colorectal cancer [32]. Similar studies have revealed that long noncoding RNA activated by TGF-β (lncRNA-ATB) upregulates ZEB1 and ZEB2 expression by competitively binding the miR-200 family, which induces epithelial-mesenchymal transition (EMT) and invasion in hepatocellular carcinoma [33].…”

Section: Discussionmentioning

confidence: 99%

The Long Non-Coding RNA XIST Controls Non-Small Cell Lung Cancer Proliferation and Invasion by Modulating miR-186-5p

Wang

Shen

Zhang

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Long non-coding RNAs (lncRNAs) are key players in the development and progression of human cancers. The lncRNA XIST (X-inactive specific transcript) has been shown to be upregulated in human non-small cell lung cancer (NSCLC); however, its role and molecular mechanisms in NSCLC cell progression remain unclear. Methods: qRT-PCR was conducted to assess the expression of XIST and miR-186. Cell proliferation was detected using MTT assay. Cell invasion and migration were evaluated using transwell assay. Cell cycle distribution and apoptosis rates were analyzed by flow cytometry. Luciferase reporter assay was used to identify the direct regulation of XIST and miR-186. A RNA immunoprecipitation was used to analyze whether XIST was associated with the RNA-induced silencing complex (RISC). Results: We confirmed that XIST was upregulated in NSCLC cell lines and tissues. Functionally, XIST knockdown inhibited cancer cell proliferation and invasion, and induced apoptosis in vitro, and suppressed subcutaneous tumor growth in vivo. Mechanistic investigations revealed a reciprocal repressive interaction between XIST and miR-186-5p. Furthermore, we showed that miR-186-5p has a binding site for XIST. Our data also indicated that XIST and miR-186-5p are likely in the same RNA induced silencing complex. Conclusion: Together, our data revealed that XIST knockdown confers suppressive function in NSCLC and XIST may be a novel therapeutic marker in this disease.

show abstract

Section: Discussionmentioning

confidence: 99%

The Long Non-Coding RNA XIST Controls Non-Small Cell Lung Cancer Proliferation and Invasion by Modulating miR-186-5p

Wang

Shen

Zhang

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Furthermore, ceRNA regulation mechanism has also been reported to play an important role in cell proliferation of esophageal cancer and CRC. [34,35] ZNF148 is over-expressed in CRC, and it inhibits cell proliferation in CRC cells. The underlying mechanism is still unknown.…”

Section: Discussionmentioning

confidence: 99%

ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer

Xian

Liu

et al. 2017

Medicine

View full text Add to dashboard Cite

Background:Competing endogenous RNA (ceRNA) regulation is a novel hypothesized mechanism that states RNA molecules share common target microRNAs (miRNAs) and may competitively combine into the same miRNA pool.Methods:Zinc finger protein 148 (ZNF148) and TOP2A expression were analyzed in 742 colorectal cancer (CRC) tissues using immunohistochemistry (IHC). ZNF148 mRNA, TOP2A mRNA, miR101, miR144, miR335, and miR365 expression were estimated in 53 fresh frozen CRC tissues by reverse transcription polymerase chain reaction. Mechanisms underpinning ceRNA were examined using bioinformatics, correlation analysis, RNA interference, gene over-expression, and luciferase assays.Results:Protein levels of ZNF148 and TOP2A detected by IHC positively correlated (Spearman correlation coefficient [rs] = 0.431, P < 0.001); mRNA levels of ZNF148 and TOP2A also positively correlated (r = 0.591, P < 0.001). Bioinformatics analysis demonstrated that ZNF148 and TOP2A mRNA had 13 common target miRNAs, including miR101, miR144, miR335, and miR365. Correlation analysis demonstrated that levels of ZNF148 mRNA were negatively associated with levels of miR144, miR335, and miR365. Knockdown and overexpression tests showed that ZNF148 mRNA and TOP2A mRNA regulated each other in HCT116 cells, respectively, but not in Dicer-deficient HCT116 cells. Luciferase assays demonstrated that ZNF148 and TOP2A regulated each other through 3′UTR. Overexpression of ZNF148 mRNA and TOP2A mRNA caused significant downregulation of miR101, miR144, miR335, and miR365 in the HCT116 cells. We also found that knockdown of ZNF148 and TOP2A significantly promoted cell growth, and overexpression of ZNF148 and TOP2A inhibited cell proliferation, which was abrogated in Dicer-deficient HCT116 cells.Conclusion:ZNF148 and TOP2A regulate each other through ceRNA regulatory mechanism in CRC, which has biological effects on cell proliferation.

show abstract

“…72 Huang et al 62 recently conducted a study that suggests that CASC2 expression was not only visibly reduced in CRC cell lines and CRC tissues but also more obviously decreased in patients with advanced CRC.…”

Section: Cancer Susceptibility Candidatementioning

confidence: 99%

Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer

Deng

Wang

et al. 2017

Tumour Biol.

View full text Add to dashboard Cite

BackgroundColorectal cancer (CRC) is the third most diagnosed cancer in this blue planet, whose incidence is increasing in young adults, especially in the developing countries. 1 As the fourth main cause of cancer-related deaths in the world, CRC contributes to a serious threat to human health. To descend mortality of CRC, in past decades, researchers strive to study the mechanisms of this disease. Unfortunately, the prognosis of this malignancy remains pessimistic in numerous patients who even received surgery, and the cancer reappear or metastasis come up after several months. One of the leading challenges of this disease is the clinical diagnosis may be late. 2 Therefore, biological biomarkers for the identification of detection and prediction may have clinical significance. Especially, in past decade, extensive molecular research has demonstrated that long non-coding RNAs (lncRNAs) may be novel and significant biological biomarkers for early diagnosis and prognosis of colon cancer. 3 As its name indicates, LncRNAs are a group of noncoding RNA molecules whose lengths are larger than 200 nucleotides and lack protein-coding capability.

show abstract

The long noncoding RNA CASC2 functions as a competing endogenous RNA by sponging miR-18a in colorectal cancer

Cited by 128 publications

References 42 publications

The Long Non-Coding RNA XIST Controls Non-Small Cell Lung Cancer Proliferation and Invasion by Modulating miR-186-5p

The Long Non-Coding RNA XIST Controls Non-Small Cell Lung Cancer Proliferation and Invasion by Modulating miR-186-5p

ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer

Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer

Contact Info

Product

Resources

About