The long non-coding RNA LSINCT5 promotes malignancy in non-small cell lung cancer by stabilizing HMGA2

Tian, Yu-Heng; Zhang, Nali; Chen, Shuwen; Ma, Yuan; Liu, Yanyan

doi:10.1080/15384101.2018.1467675

Cited by 29 publications

(23 citation statements)

References 35 publications

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“…Subsequently, Silva et al [12] further found LSINCT5 expression levels were obviously increased in breast and ovarian cancer tissues and cell lines compared with their corresponding normal tissues and cell lines, respectively. Recently, LSINCT5 overexpression has been identified in lung cancer [13], hepatocellular carcinoma [14], bladder cancer [15], gastric cancer [16,17] and colorectal cancer [16]. In our study, we also found LSINCT5 expression was increased in osteosarcoma tissue samples and cell lines, which was similar to recent study reported by Kong et al [10].…”

Section: Discussionsupporting

confidence: 91%

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

Ming

Dongbo

2019

Bioscience Reports

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The dysregulated expression of LSINCT5 (long stress-induced non-coding transcript 5) has been found in various human tumors, and was generally related to cancer progression and unfavorable prognosis. Although the role of LSINCT5 in osteosarcoma was reported not long ago, the sample size of that study was limited. Our study presented more evidence about the clinical significance and biological function of LSINCT5 in osteosarcoma. In our results, we found LSINCT5 expression was increased in osteosarcoma tissue samples and cell lines, and high LSINCT5 expression was associated with advanced Enneking stage, large tumor size, high histological grade and present distant metastasis. Meanwhile, we observed high LSINCT5 expression was correlated with worse overall survival, and high LSINCT5 expression could be an independent poor predictor for overall survival in osteosarcoma cases. Moreover, we found inhibition of LSINCT5 expression suppressed cell proliferation, migration and invasion in vitro, and LSINCT5 overexpression dramatically facilitated cell proliferation, migration and invasion in vitro. In conclusion, our study suggests that LSINCT5 exerts oncogenic function in osteosarcoma cells, and may be a potential predictor for clinical outcome in osteosarcoma patients.

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Section: Discussionsupporting

confidence: 91%

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

Ming

Dongbo

2019

Bioscience Reports

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“…The low survival rate of OS is mainly due to the fact that patients are prone to focus metastasis. Previous studies have shown that LSINCT5 can inhibit the metastasis of lung cancer by regulating HMGA2, 24 while our research has found that LSINCT5 also has the function of inhibiting tumor cell metastasis in OS, which has indicated that LSINCT5 has the function of inhibiting tumor metastasis in various tumors. In order to further explore the mechanism of LSINCT5, we predicted the miR that LSINCT5 could bind to it.…”

Section: Discussionmentioning

confidence: 44%

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

Liao

Guan

et al. 2020

OTT

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Background: More and more evidence has shown that non-coding RNA (ncRNA), including long ncRNA (lncRNA) and micro RNA (miRNA), plays a crucial regulatory role in osteosarcoma (OS). Previously, we revealed a Rho-related coiled coil incorporating protein kinase 1(XIAP). A transfer-related gene is negatively regulated by microRNA-20a-5p (miR-20a-5p) and plays the role of oncogene in OS. It is not clear if any lncRNA is involved in the axial upstream of miR-20a-5p/XIAP. Methods: Expression of LSINCT5 and miR-20a-5p/XIAP in OS tissues was determined through qRT-PCR (qP). The proliferation and migration/invasion activity of OS cells were tested through CCK-8/and transwell assay, respectively. The changes on expression of XIAP were examined through qRT-PCR and Western blot (WB). Targeted binding between LSINCT5, miR-20a-5p, and XIAP has been verified using dual luciferase reporter gene analysis, RNA Immunoprecipitation (RIP), and RNA pull-down experiments. The effect of LSINCT5 on tumor growth was determined by tumor allograft test. Results: In this study, elevated LSINCT5 was found in OS tissue samples and OS cell strains, and the increased LSINCT5 was strongly related to the adverse prognosis of clinical patients. Functional assays showed that inhibition of LSINCT5 could up-regulate miR-20a-5p-mediated OS cells proliferation and metastasis. WB analysis and qP analysis showed that LSINCT5 regulated XIAP by mediating miR-20a-5p. Further cell behavior experiments showed that LSINCT5 acted as a miR-20a-5p sponge to inhibit proliferation and metastasis caused by XIAP. Finally, the results of animal models in vivo showed that LSINCT5 could regulate the tumor growth of OS. Conclusion: LncRNA LSINCT5 acts as an oncogene and promotes XIAP mediated growth and metastasis as competitive endogenous RNA (ceRNA) in OS.

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“…In addition, there is a current lack of highly sensitive and specific markers for the diagnosis of early-stage LUAD. Recently, long non-coding RNAs (lncRNAs) have become novel and popular markers for detecting tumors in the bloodstream, which could allow for the dynamic monitoring of tumor cell invasion, activity and patient prognosis (Tian et al, 2018b; Zhao et al, 2018). In addition, lncRNAs have been found to exert multiple functional roles in lung cancer (Li et al, 2018; Qian et al, 2018; Yu et al, 2018; Zhang et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Identification of a putative competitive endogenous RNA network for lung adenocarcinoma using TCGA datasets

Wang

et al. 2019

PeerJ

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The mechanisms underlying the oncogenesis and progression of lung adenocarcinoma (LUAD) are currently unclear. The discovery of competitive endogenous RNA (ceRNA) regulatory networks has provided a new direction for the treatment and prognosis of patients with LUAD. However, the mechanism of action of ceRNA in LUAD remains elusive. In the present study, differentially expressed mRNAs, microRNAs (miRs) and long non-coding RNAs from the cancer genome atlas database were screened. CeRNAs for LUAD were then identified using online prediction software. Among the ceRNAs identified, family with sequence similarity 83 member A (FAM83A), miR-34c-5p, KCNQ1OT1 and FLJ26245 were observed to be significantly associated with the overall survival of patients with LUAD. Of note, FAM83A has potential significance in drug resistance, and may present a candidate biomarker for the prognosis and treatment of patients with LUAD.

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The long non-coding RNA LSINCT5 promotes malignancy in non-small cell lung cancer by stabilizing HMGA2

Cited by 29 publications

References 35 publications

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

Identification of a putative competitive endogenous RNA network for lung adenocarcinoma using TCGA datasets

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