LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

He, Wenbo; Ming, Liao; Dongbo, Xiao

doi:10.1042/bsr20190612

Cited by 10 publications

(9 citation statements)

References 18 publications

(34 reference statements)

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“…Previous research has shown that LSINCT5 affects the development and progression of colorectal carcinoma, ovarian cancer, 20 gastric cancer, 21 esophageal cancer, and chronic heart failure, 22,23 but there is relatively little research on OS at present. However, our study revealed that LSINCT5 was highly expressed in OS tissues and cell strains, and the 5-year survival of patients with high expression decreased, which was consistent with the study of He et al 12 However, the relevant mechanism is still unclear. Therefore, we conducted tests to further seek the potential mechanism of LSINCT5 in OS.…”

Section: Discussionsupporting

confidence: 91%

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<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

Liao

Guan

et al. 2020

OTT

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Background: More and more evidence has shown that non-coding RNA (ncRNA), including long ncRNA (lncRNA) and micro RNA (miRNA), plays a crucial regulatory role in osteosarcoma (OS). Previously, we revealed a Rho-related coiled coil incorporating protein kinase 1(XIAP). A transfer-related gene is negatively regulated by microRNA-20a-5p (miR-20a-5p) and plays the role of oncogene in OS. It is not clear if any lncRNA is involved in the axial upstream of miR-20a-5p/XIAP. Methods: Expression of LSINCT5 and miR-20a-5p/XIAP in OS tissues was determined through qRT-PCR (qP). The proliferation and migration/invasion activity of OS cells were tested through CCK-8/and transwell assay, respectively. The changes on expression of XIAP were examined through qRT-PCR and Western blot (WB). Targeted binding between LSINCT5, miR-20a-5p, and XIAP has been verified using dual luciferase reporter gene analysis, RNA Immunoprecipitation (RIP), and RNA pull-down experiments. The effect of LSINCT5 on tumor growth was determined by tumor allograft test. Results: In this study, elevated LSINCT5 was found in OS tissue samples and OS cell strains, and the increased LSINCT5 was strongly related to the adverse prognosis of clinical patients. Functional assays showed that inhibition of LSINCT5 could up-regulate miR-20a-5p-mediated OS cells proliferation and metastasis. WB analysis and qP analysis showed that LSINCT5 regulated XIAP by mediating miR-20a-5p. Further cell behavior experiments showed that LSINCT5 acted as a miR-20a-5p sponge to inhibit proliferation and metastasis caused by XIAP. Finally, the results of animal models in vivo showed that LSINCT5 could regulate the tumor growth of OS. Conclusion: LncRNA LSINCT5 acts as an oncogene and promotes XIAP mediated growth and metastasis as competitive endogenous RNA (ceRNA) in OS.

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Section: Discussionsupporting

confidence: 91%

“…Previous studies have found that LSINCT5 can be used as a prognostic indicator for OS and plays a vital part in tumor carcinogenesis. 12 Nevertheless, the relevant mechanism of LSINCT5 in OS is still unclear.…”

Section: Introductionmentioning

confidence: 99%

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

Liao

Guan

et al. 2020

OTT

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“…The expression levels of long stress-induced noncoding transcript 5 (LSINCT5) were upregulated in osteosarcoma tissues and cell lines. High LSINCT5 level was positively correlated with malignant clinicopathological features, including advanced Enneking stage and histological grade, larger tumor size, and distant metastasis [ 151 ]. Besides, osteosarcoma patients with high expression of LSINCT5 showed a trend toward decreased overall survival [ 151 , 152 ].…”

Section: The Clinical Significance Of Lncrnas Involved In Wnt Pathway In Osteosarcomamentioning

confidence: 99%

Functional interplay between long non-coding RNAs and the Wnt signaling cascade in osteosarcoma

Lin

Liu

et al. 2021

Cancer Cell Int

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Osteosarcoma is a common and highly malignant bone tumor among children, adolescents and young adults. However, the underlying molecular mechanisms remain largely unexplored. LncRNAs are transcripts with no or limited protein-coding capacity in human genomes, and have been demonstrated to play crucial functions in initiation, progression, therapeutic resistance, recurrence and metastasis of tumor. Considerable studies revealed a dysregulated lncRNA expression pattern in osteosarcoma, which may act as oncogenes or suppressors to regulate osteosarcoma progression. Wnt signaling pathway is an important cascade in tumorigenesis by modulation of pleiotropic biological functions including cell proliferation, apoptosis, differentiation, stemness, genetic stability and chemoresistance. Hyperactivation or deficiency of key effectors in Wnt cascade is a common event in many osteosarcoma patients. Recently, increasing evidences have suggested that lncRNAs could interplay with component of Wnt pathway, and thereby contribute to osteosarcoma onset, progression and dissemination. In this review, we briefly summarize Wnt signaling-related lncRNAs in osteosarcoma progression, aiming to gain insights into their underlying crosstalk as well as clinical application in osteosarcoma therapeutic modalities.

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“…Thus, new candidate molecules as prognostic biomarkers are expected to be developed, which may profoundly improve clinical outcomes for patients with OS. Encouragingly, some long non-coding RNAs (lncRNAs) have been reported to serve as predictive, prognostic, and therapeutic biomarkers of human cancers recently, since they are usually aberrantly expressed in disease tissues and promote or inhibit tumor development, progression, and metastasis [8][9][10][11]. Altering from the perception as 'junk' transcriptional products, lncRNAs have been recognized to play cellfunction regulatory roles and mediate cellular processes including transcription, post-transcriptional modifications, and signal transduction [12].…”

Section: Introductionmentioning

confidence: 99%

Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function

Dong

Wang

et al. 2021

J Orthop Surg Res

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Background Osteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function. Methods One hundred eight pairs of tissues were collected, and OS cell lines were purchased. lncRNA GNAS-AS1 expression in these tissues and cells were analyzed by qRT-PCR. Clinical data were analyzed using chi-square tests, Kaplan-Meier curves (log-rank test), and Cox regression. CCK-8 and transwell assay were conducted to analyze the effect of lncRNA GNAS-AS1 on cell proliferation, invasion, and migration. The downstream miRNA was presumed. Results The expression of lncRNA GNAS-AS1 was significantly increased in OS cells and tissues, and related to Enneking staging and distant metastasis. Patients with high lncRNA GNAS-AS1 expression represented shorter overall survival and was an independent prognostic predictor of OS. LncRNA GNAS-AS1 knockdown inhibited cell proliferation, migration, and invasion by regulated miR-490-3p partly at least. Conclusions LncRNA GNAS-AS1 can be used as a prognostic indicator and its inhibition suppress the development of OS, suggesting its value as novel therapeutic strategies in OS.

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LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

Cited by 10 publications

References 18 publications

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

Functional interplay between long non-coding RNAs and the Wnt signaling cascade in osteosarcoma

Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function

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