The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene

Baculoviruses contain a gene for the large subunit of ribonucleotide reductase.

“…The sequence surrounding the major transcriptional start sites showed no homology to previously characterized start sites of other baculovirus genes, for instance those involved in DNA replication, such as helicase (Lu & Carstens, 1992) and DNA polymerase (Tomalski et al, 1988). No homology between the sequences 5h of the translational start of SeMNPV, SpliMNPV and other baculovirus rr1 genes could be detected.…”

The p6.5 gene region of a nuclear polyhedrosis virus of Orgyia pseudotsugata: DNA sequence and transcriptional analysis of four late genes

“…4d) , BV envelope glycoprotein (gp64) , polyhedron envelope protein (PE) (Gombart et al, 1989) and pl0 (Leisy et al, 1986) genes. The location of the OpMNPV DNA polymerase gene was determined by hybridization of the AcMNPV SmaI/PstI fragment (map units 40.3 to 42; Tomalski et al, 1988) bp insert) Asp718 fragment derived from a PstI clone (extending from the PstI site at nt 3006 in Fig. 2 to 2.45 kb downstream) and which contains the 3' end of p6.5 ( Fig.…”

Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).

“…Six of these genes appear to be required for DNA replication and encode the following proteins : a DNA polymerase homologue (Tomalski et al, 1988) ; P143, a helicase homologue (Lu & Carstens, 1991) that is capable of binding dsDNA (Laufs et al, 1997) ; LEF-3, a homotrimeric ssDNA-binding (SSB) protein Hang et al, 1995) that appears to be involved in transporting P143 into the nucleus (Wu & Carstens, 1998) ; IE-1, a transcriptional activator (Guarino & Summers, 1987) which also binds to hrs (Choi & Guarino, 1995 ;Guarino & Dong, 1991 ;Kovacs et al, 1992 ;Leisy et al, 1995 ;Rodems & Friesen, 1995) ; and two proteins, LEF-1 (a putative primase) and LEF-2, which have been shown to interact with each other . The three gene products, which are stimulatory for DNA replication, include two additional transcriptional activators, IE-2 (Carson et al, 1991) and PE-38 (Krappa & Knebel-Mo$ rsdorf, 1991), and P35, which blocks apoptosis (Clem et al, 1991 ;Herschberger et al, 1992) and may therefore influence DNA replication in an indirect manner.…”